Background
Methods
Non-human primate samples
Code | Species | Parasitaemia (parasites/μL) | PCRa: whole blood | PCRa: 1st blood clot DNA extractionb | PCRa: 2nd blood clot DNA extractionb | PCRa: 3rd blood clot DNA extractionb |
---|---|---|---|---|---|---|
H1 |
Homo sapiens
| 208 |
Plasmodium; P. vivax/P. simium
|
Plasmodium; P. vivax/P. simium
|
Plasmodium; P. vivax/P. simium
| Plasmodium; P. vivax/P. simium |
H2 |
Homo sapiens
| 368 |
Plasmodium; P. vivax/P. simium
|
Plasmodium; P. vivax/P. simium
|
Plasmodium; P. vivax/P. simium
|
Plasmodium; P. vivax/P. simium
|
H3 |
Homo sapiens
| 0 | − | − | − | − |
M1 |
Alouatta g. clamitans
| 300 | Plasmodium; P. vivax/P. simium and P. malariae/P. brasilianum | Plasmodium; P. vivax/P. simium and P. malariae/P. brasilianum |
Plasmodium; P. vivax/P. simium and P. malariae/P. brasilianum
|
Plasmodium; P. vivax/P. simium and P. malariae/P. brasilianum
|
M2 | Callithrix hybrid | 0 | − | − | − | − |
M3 |
Alouatta g. clamitans
| 40 |
P. malariae/P. brasilianum
|
P. malariae/P. brasilianum
|
P. malariae/P. brasilianum
|
P. malariae/P. brasilianum
|
Human samples
Parasitological diagnosis
DNA extraction and PCR
Sensitivity comparison assay
M3 Alouatta g. clamitans | Dilutions | ||||||||
---|---|---|---|---|---|---|---|---|---|
Conditions | Sample | 1 | 1:2 | 1:4 | 1:10 | 1:100 | 1:1000 | 1:10,000 | 1:1,000,000 |
Regular PCRa conditions | Blood clotb |
+
|
+
|
+
|
+
|
−
|
−
|
−
|
−
|
Whole blood |
+
|
+
|
+
|
+
|
+
|
−
|
−
|
−
| |
Improved PCRa conditions | Blood clotb | + | + | + | + | + |
−
|
−
|
−
|
Whole blood | + | + | + | + | + | + |
−
|
−
|