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Inflammation Research

Erschienen in:

29.07.2024 | Original Research Paper

Function of unconventional T cells in oral lichen planus revealed by single-cell RNA sequencing

verfasst von: Chen Zhao, Ruowen Zhao, Xinwen Wu, Kailin Tang, Pan Xu, Xin Chen, Pingyi Zhu, Yuan He

Erschienen in: Inflammation Research | Ausgabe 9/2024

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Abstract

Objective

We intended to map the single-cell profile of OLP, explore the molecular characteristics of unconventional T cells in OLP tissues.

Methods

Buccal mucosa samples from OLP patients and healthy individuals were used to prepare single-cell suspension. Single-cell RNA sequencing was used to analyze the proportion of all the cells, and the molecular characteristics of unconventional T cells. Immunohistochemical staining was used to detect the expression of unconventional T cells marker genes.

Results

The cell clusters from buccal mucosa were categorized into immune cells, fibroblasts, endothelial cells, and epithelial cells. Unconventional T cells with phenotype of CD247⁺TRDC⁺NCAM1⁺ were identified. Immunohistochemical staining revealed higher expression of unconventional T cell marker genes in OLP tissue, predominantly in the lamina propria. In OLP, unconventional T cells are in a unique stress response state, exhibited enhanced NF-κB signaling and apoptosis inhibition, enhanced heat shock protein genes expression, weakened cytotoxic function. A large number of ligand-receptor pairs were found between unconventional T cells and other cells, particularly with fibroblasts and endothelial cells.

Conclusions

This study mapped the single-cell profile of OLP, delineated the molecular characteristics of unconventional T cells in OLP, and uncovered that these unconventional T cells are in a stress response state.

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Titel: Function of unconventional T cells in oral lichen planus revealed by single-cell RNA sequencing
verfasst von: Chen Zhao
Ruowen Zhao
Xinwen Wu
Kailin Tang
Pan Xu
Xin Chen
Pingyi Zhu
Yuan He
Publikationsdatum: 29.07.2024
Verlag: Springer International Publishing
Erschienen in: Inflammation Research / Ausgabe 9/2024
Print ISSN: 1023-3830
Elektronische ISSN: 1420-908X
DOI: https://doi.org/10.1007/s00011-024-01912-6

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Function of unconventional T cells in oral lichen planus revealed by single-cell RNA sequencing