Background
Methods
Study population
Sample collection, RNA and DNA extraction
Polymorphism detection
Position | Method | Primer Sequences | Allele Phenotypes |
---|---|---|---|
−3279 A > C (rs3761548) | Forward: CTGGCTCTCTCCCCAACTGA Forward: TGGCTCTCTCCCCAACTGC Common Reverse: ACAGAGCCCATCATCAGACTCTCTA | A: 334 bp C: 333 bp | |
−924 A > G (rs2232365) | PCR-SSP | Forward: CCCAGCTCAAGAGACCCCA Reverse: GGGCTAGTGAGGAGGCTATTGTAAC Forward: CCAGCTCAAGAGACCCCG Reverse: GCTATTGTAACAGTCCTGGCAAGTG | A: 442 bp G: 427 bp |
FoxP3 gene | qRT- PCR | Forward: CAGCTGCCCACACTGCCCCTAG Reverse: CATTTGCCAGCAGTGGGTAG | |
GAPDH gene | Forward: CCAGGTGGTCTCCTCTGACTTCAACA Reverse: AGGGTCTCTCTCTTCCTCTTGTGCTCT |
FOX3 gene expression analysis
Statistical analysis
Results
Comparison of FoxP3 genotypes (−924 A > G, −3279 A > C) in both case and control groups
Position | Gender (No) | Genotype/Allele | Controls (%)No | TB patient No (%) | Odd Ratioa (95% CI) | Pb |
---|---|---|---|---|---|---|
FoxP3
−3279 A > C | Female | AA AC CC | 38 (53.2) 20 (28.1) 13 (18.3) | 43 (51.1) 15 (17.8) 26 (30.9) | 1:00 (Reference)c
0.56 (0.25 ± 1.2) 1.5 (0.67 ± 3.35) | 0.1 |
Male | A C | 65 (58.03) 47 (41.9) | 64 (64.6) 35 (35.3) | 1:00 (Reference) 1.3 (0.75 ± 2.3) | 0.3 | |
Total | A C | 161 (63.3) 93 (36.6) | 165 (61.7) 102 (38.2) | 1:00 (Reference) 0.93 (0.65 ± 1.3) | 0.7 | |
FoxP3
−924 A > G | Female | AA AG GG | 34 (47.8) 14 (19.7) 23 (32.3) | 19 (22.6) 22 (26.19) 43 (51.1) | 1:00 (Reference) 0.29 (0.14 ± 0.63) 0.35 (0.14 ± 0.85) | 0.005 |
Male | A G | 73 (65.1) 39 (34.8) | 48 (48.4) 51 (51.5) | 1:00 (Reference) 3.42 (2.17 ± 5.39) | 0.01 | |
Total | A G | 155 (61) 99 (39) | 108 (40.4) 159 (59.5) | 1:00 (Reference) 0.43(0.3 ± 0.61) | 0.00 |