Backgrounds
Pulmonary arterial hypertension (PAH) refers to the first subgroup of pulmonary hypertension (PH) patients, which is defined as a pulmonary arterial wedge pressure (PAWP) ≤ 15 mmHg and an indexed pulmonary vascular resistance (PVRI) greater than 3 Wood units [
1]. Although the use of targeted drugs (such as endothelin receptor antagonists, prostacyclin inhibitors, etc.) has increased the survival rate of PAH patients and significant improvements in PAH outcome have been realized in the modern era (median survival 7 years) compared to the National Institutes of Health registry from the early 1980s (median survival 2.8 years) [
2], its long-term prognosis remains poor. The aetiology of PAH involves environmental and genetic factors, and its pathogenesis is complex and has not been fully elucidated to date..
Bone morphogenetic protein (BMP), a multifunctional protein, was originally identified as an osteoinductive component in extracts derived from bone. BMPs play important roles through BMP receptors (BMPRs) in a multitude of processes during embryonic development and adult homeostasis. BMPRs are serine/threonine kinase receptors composing an intracellular serine/threonine kinase domain, a single transmembrane domain, and a short extracellular domain containing 10–12 cysteine residues. The BMP type II receptor (BMPRII), activin type II receptor (ActRII), and activin type IIB receptor (ActRIIB) are three type II BMPRs present in mammals. Autosomal dominant mutation causing haploinsufficiency or loss of function of BMPR2 is the most common cause of PAH. Studies have reported more than 298 BMPR2 mutations are responsible for 55 to 70% of heritable PAH (HPAH) and 11 to 40% of idiopathic PAH (IPAH) [
3]. What’s more, the BMPR2 signaling pathway is also impaired in PAH patients without BMPR2 mutations [
4]. Despite the impact of BMPR2 as the main genetic factor for PAH, currently, our understanding of the specific mechanisms of BMPR2 in PAH is incomplete. Unravelling these uncertainties may be could explain why only 20% of BMPR2 mutation carriers develop advanced PAH [
5] and might predict which mutation carriers will further develop PAH. It is now recommended by the European guidelines for the management of PAH that patients recently diagnosed with idiopathic, heritable, or anorexigen-associated PAH should be offered genetic counselling and screening for BMPR2 mutations, mainly to enable predictive genetic testing of relatives. Studies have suggested that patients with PAH who carry causal BMPR2 mutations may present at an earlier age and have more severe haemodynamic compromise [
6]. Additionally, there is a “sex paradox” exists in PAH while women are more likely than men to develop PAH, men have worse outcomes than those observed in their opposite sex counterparts [
7]. Therefore, among PAH patients, the question of whether there is a gender difference in BMPR2 mutation is still unclear. In this article, we sought to combine recent clinical research investigating BMPR2 and PAH to present an evidence-based meta-analysis and explain the scientific phenomenon of gender differences in the development of PAH disease. Moreover, these data provide clinical guidance for the treatment of gender-based differences in PAH.
Discussion
Of all BMPRs, BMPR2 is the most relevant to PAH. BMPR2 mutations were the first BMPR mutations to be discovered, and they are the most extensively studied mutations of those known to underlie HPAH. Transgenic mice with PAH expressing a dominant-negative BMPRII gene display aberrant pulmonary vascular cell phenotypes, including apoptosis of endothelial cells (ECs) and excessive proliferation of medial smooth muscle cells (SMCs) [
25]. Metabolomic analysis demonstrated that BMPR2 mutations were associated with a wide range of metabolic abnormalities, including oxidative injury and insulin resistance in human pulmonary ECs. Furthermore, BMPR2 deficiency aggravates endothelial inflammatory responses, thereby contributing to adverse vascular remodeling [
26]. Homozygous BMPR2(−/−) knockout mouse died in utero whereas heterozygous BMPR2(+/−) mice were viable but did not develop PAH spontaneously, even in the presence of a second hit such as hypoxia, mouse did not develop severe forms of PAH [
27]. Given that BMP ligands and their receptors play important roles in disease progression, the regulation of this signal could function as a therapeutic target.
As mentioned earlier, there is a “sex paradox” in PH that it has long been known females have a higher susceptibility than males to PAH, in which the most recent figures show that the female-to-male ratio is 4:1 [
28]. In a large cohort of individuals with BMPR2 receptor mutations (including those with IPAH or HPAH or drug- and toxin-induced PAH), approximately 70% of the patients were women [
6]. Similar to previous studies, in our study, 457 of 681 PAH patients with BMPR2 mutations were women. Interestingly, among all female PAH patients, the proportion who had BMPR2 mutations was lower than the proportion of male PH patients with BMPR2 mutations out of all male PAH patients. Therefore, our and previous studies suggest that the pathogenesis of PAH may be more complicated in female than male PAH patients, and that the influence of BMPR2 mutations may be modified by additional unknown factors in female patients. A large and growing body of literatures have investigated the cross-talk between BMPR2 and oestrogen signaling, which has been proposed as a critical mechanistic driver responsible for the female predominance of PAH. Austin ED ect. Showed that when oestrogen receptor alpha binds to the BMPR2 promoter, BMPR2 gene expression was reduced in females [
29]. Mair and colleagues examined the expression of aromatase (a member of the cytochrome P-450 superfamily that synthesizes oestrogens via the aromatization of androgens) in human pulmonary artery smooth muscle cells (hPASMCs) and demonstrated that the level of aromatase was 12-fold higher in cells derived from postmenopausal women than in PASMCs derived from similarly aged men [
30]. Furthermore, a large number of recent studies have focused on differences in hormone levels but have ignored the most fundamental difference between males and females: the sex chromosomes (XX versus XY). Yan and colleagues demonstrated that SRY on the Y chromosome binds to and positively regulates the BMPR2 promoter to reduce the prevalence of PAH in males [
31]. Moreover, studies have demonstrated mitochondria also play an important role in PAH [
32]. Does female mitochondria also be differ from male mitochondria? It’s true! Mitochondria are passed to the offspring only from mother. Female mitochondria can respond to evolutionary pressure because they are passed on to the offspring. However, male mitochondria are not responsive to such pressures because they are locked in the host cell and cannot be passed on [
33].
Studies have increasingly shown that inflammation plays an important role in PAH and the inflammatory insult is also considered as a second hit potential triggering the pathogenesis of PAH [
34]. The occurrence of PAH is related to various inflammatory factors, such as systemic inflammatory response, human immunodeficiency virus infection, autoimmune disease, etc. Evidence suggesting that inflammation is involved in the development of PAH was first presented in 1994. Tuder found that macrophages, T cells, B cells and other inflammatory cells infiltrate surrounding injured vessels in PAH patients with pulmonary plexiform lesions [
35], and many studies have found significantly increased levels of inflammatory markers in the blood of PAH patients, including C-reactive protein (CRP), interleukins (IL, such as IL-1, IL-6, IL-8, and IL-10), monocyte chemotactic protein 1 (MCP-1), tumor necrosis factor-α (TNF-α), and high mobility group box chromosomal protein 1 (HMGB1) [
36]. Furthermore, differences in the immune system have been identified between men and women. Therefore, we predict that these sex-related differences in the immune system contribute to the PH “sex paradox”. In addition to IPAH and HPAH, other World Health Organization (WHO) Group 1 PAH subgroups are also characterized by a female predominance [
7], including connective tissue disease (CTD) and portopulmonary hypertension-associated PAH. CTD is more common in women than men, and when associated with PAH, female/male ratios between 3.8:1 and 10:1 have been reported [
10]. In systemic sclerosis, women are eight times more likely than men to be affected by PAH, and in systemic lupus erythematosus, women are 17 times more likely than men to be affected by PAH [
8].
Our meta-analysis further confirmed a positive correlation between BMPR2 mutations and the severity of PAH, which is consistent with previous studies [
9], reporting that PAH patients carrying BMPR2 mutations have a higher mean pulmonary artery pressure and a lower cardiac index. Similar to the Evans study, we also found that in patients with PAH, the risk of death or transplantation was higher among those with BMPR2 mutations than those without [
6]. However, it is worth noting that when we conducted subgroup analyses according to gender to explore the sources of heterogeneity, we found that the difference was significant only in male patients but not in female patients. To explain this finding, we first focused on differences in sex hormones between males and females. Similar to the “sex paradox”, there are also “oestrogen paradoxes” in PAH [
7]. At low doses, oestrogen acts as a pro-oxidant, whereas at higher doses, it acts to suppress oxidative stress. In addition to its dose-effect, studies have demonstrated that some oestrogen metabolites (such as 17-β oestradiol and 2-OH-estradiol) can also promote antiproliferative and proapoptotic signals, inhibit oxidative stress and collagen deposition, and protect right ventricular function in PAH, while other metabolites (such as 16-α-OH-oestradiol) have the opposite effects [
28]. The effects of key enzymes in the lungs (such as CYP1B1 and catechol-O-methyl-transferase (COMT)) have also been explored. CYP1B1 is the most efficient oestradiol hydroxylase, and the 2-OH-estradiol/16α-OH-estrone ratio is an indicator of CYP1B1 activity [
37]. In the presence of COMT, 2-Methoxy-estradiol is rapidly formed. The imbalance of oestrogen metabolites has been proposed to be the basis of the differential effects of oestrogens on male versus female pulmonary vascular cells [
38]. As mentioned earlier, in addition to hormonal factors, there are also differences in the immune system between men and women. Some studies have demonstrated that Tregs are more critical for the maintenance of immune homeostasis in females than in males. Treg cells can maintain immune homeostasis and suppress inflammatory responses by regulating the function of effector T cells. Under physiological conditions, Th17 and Treg are in a dynamic equilibrium. In PAH, the balance of Th17 and Treg is broken, and Th17 cells, which have pro-inflammatory effects, are increased, and the activation of Treg cells, which exert anti-inflammatory protective effects, is reduced [
39]. Osman and colleagues found that Tregs suppressed inflammation, immune dysregulation and vascular remodeling in females and that they thereby exert a protective effect in PAH models [
33]. Moreover, metabolic theory suggests that vascular cell mitochondria can induce a proliferative, antiapoptotic phenotype and the inflammasome, thus initiating a cascade of events that increases the levels of many inflammatory cytokines described in PAH [
40]. Hence, female mitochondria may be different from male mitochondria. Oocytes cell mitochondria have different ultrastructures, slower metabolic activity and lower levels of ROS production than those observed in sperm cell mitochondria. Importantly, mitochondria can strongly regulate the functions of T cells. For example, mitochondrial suppression of oxidative phosphorylation is a necessary step preceding T cell activation [
41].
Our results also indicate that screening for BMPRR2 mutations is more important in men than in women. First, we presented evidence from 17 published clinical trials in this review and suggested that among 2198 PAH patients, BMPR2 mutations accounted for a higher proportion of all factors leading to PAH in men than women. Second, the PAH patients with BMPR2 mutations had more severe haemodynamic and functional parameters than noncarriers, and the carriers were diagnosed at younger ages. The risk of death or transplantation in PAH patients with BMPR2 mutations was higher than that in those without such mutations. The third and last, the difference was significant only in male patients. Therefore, treatment of aberrant BMPR2 expression may exert better effects in male IPAH and HPAH patients than in female patients. This study also has some shortcomings. We found that male PAH patients are more likely than female patients to have BMPR2 mutations, but whether this difference is related to the severity of male PAH has not been further explored. The main reason is that many end points of the included clinical studies were only systematically compared with regard to the presence or absence of BMPR2 mutations, and no stratified analysis of males and females has been performed. We recommend a previously completed or ongoing trial or registration of PAH should be analyzed in a gender subgroup analysis of patients with BMPR2 mutations to determine whether gender differences in BMPR2 mutations are associated with gender differences in disease severity among this patient population. Moreover, some heterogeneity was observed in the mortality or lung transplantation rates among the trials. In Isobe’s study, we observed that the overall results were consistent with other studies. The all-cause mortality was similar between the BMPR2 carriers and non-carriers, but in contrast to the other included studies, this study found that the all-cause mortality was higher among the BMPR2 non-carriers. The reasons may be summarized as follows: the population explored in this study were treated with PGI
2, while the other studies did not involve treatment, and 13.6% of the entire cohort in this study had familial pulmonary hypertension, which is twice the 6% reported in the U.S. registry conducted in the 1980s. Thus, we cannot deny the possibility that ethnicity contributes to the impact of BMPR2 mutations on the outcome. Indeed, the heterogeneity was greatly reduced after eliminating Isobe’s study (from 64 to 49%, data not shown). Thus, a worldwide, large-scaled prospective study should be performed in the near future to elucidate the differences in among response to the currently available combination therapies between for BMPR2. What’s more, there are studies have reported that gender-based differences in PAH prevalence appear to be diminished among older patients [
42]. Therefore, an another important modifier of the relationship between gender and outcomes in PAH may be is age, which suggests that temporal changes in the hormonal milieu may impact disease risk and severity throughout the lifespan [
43]. Just as we discussed before, race/ethnicity may also modify the relationship between sex and PAH. So, we probably group it more finely, such as by region, age, etc., to further explain the high heterogeneity.
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