Background
Methods
Collection of cumulus-oocyte complexes
Culture of cumulus-oocyte complexes in vitro
Microarray
Quantitative real-time PCR
Results
Microarray analysis
Gene ontology
Real-time RT-PCR validation
Probe name | Gene symbol | Gene description | Fold change in gene expression values determined by RT-PCR (Mean ± SEM) | Significance vs. non-stimulated control | Array results (Fold change) |
---|---|---|---|---|---|
FSH vs. Control - overexpressed | |||||
31605:45360_15417313:f |
ABCC5
| ATP-binding cassette, sub-family C (CFTR/MRP), member 5 | 0.83 ± 0.12 | ns. | 4.79 |
9436:30168_CL9Contig1:f |
ADAMTS1
| ADAM metallopeptidase with thrombospondin type 1 motif, 1 | 3.72 ± 0.36 | *** | 6.95 |
13062:45360_CL23Contig1:f |
ANXA1
| Annexun A1 | 0.93 ± 0.10 | ns. | 3.15 |
1117:45360_CL10Contig1:r |
ANXA2
| Annexin A2 | 0.98 ± 0.07 | ns. | 4.02 |
14127:9388_21549088:r |
AREG
| Amphiregulin | 5.04 ± 0.46 | *** | 4.06 |
18133:7423_CL1Contig2:r |
CEBPB
| CCAAT/enhancer binding protein (C/EBP), beta | 9.68 ± 1.58 | *** | 2.89 |
6532:35657_CL1Contig1:r |
CYP11A1
| Cytochrome P450, family 11, subfamily A, polypeptide 1 | 11.92 ± 0.90 | *** | 16.20 |
TC226972:f |
FOXO3
| Forkhead box O3 | 1.97 ± 0.21 | ** | 4.22 |
TC235800:f |
HAS2
| Hyaluronan synthase 2 | 47.63 ± 5.11 | *** | 6.59 |
9355:35407_CL1Contig1:f |
HSD17B7
| Hydroxysteroid (17-beta) dehydrogenase 7 | 3.35 ± 0.30 | *** | 3.87 |
5795:24780_CL1Contig1:r |
MARCKSL1
| MARCKS-like 1 | 4.44 ± 0.53 | *** | 2.85 |
1337:12192_CL1Contig2:f |
ODC1
| Ornithine decarboxylase 1 | 6.22 ± 0.85 | ** | 5.26 |
15979:340_CL1Contig1:f |
PBX1
| Pre-B-cell leukemia homeobox 1 | 0.70 ± 0.10 | * | 4.24 |
31453:2453_233792:f |
PGR
| Progesterone receptor | 23.99 ± 5.10 | ** | 15.57 |
8538:3878_CL2Contig1:f |
PLAT
| Plasminogen activator, tissue | 14.16 ± 2.36 | *** | 5.48 |
4143:1539_CL1Contig1:r |
PLAUR
| Plasminogen activator, urokinase receptor | 2.56 ± 0.20 | *** | 4.00 |
33691:1766_29276590:r |
PRDX2
| Peroxiredoxin 2 | 6.30 ± 0.44 | *** | 2.88 |
13439:18092_CL1Contig1:r |
RBKS
| Ribokinase | 0.51 ± 0.07 | *** | 7.32 |
3373:854_CL1Contig1:f |
RGS2
| Regulator of G-protein signaling 2 | 3.47 ± 0.77 | * | 3.13 |
33911:29637_CL1Contig1:r |
SBSN
| Suprabasin | 20.37 ± 1.56 | *** | 21.54 |
TC204140:f |
SDC1
| Syndecan 1 | 2.55 ± 0.57 | * | 13.44 |
2269:10415_CL1Contig1:f |
SDC4
| Syndecan 4 | 13.79 ± 1.71 | *** | 6.29 |
TC238171:f |
SERPINE1
| Serpin peptidase inhibitor, clade E, member 1 | 3.05 ± 0.50 | * | 14.47 |
31918:32605_CL1Contig2:f |
SMOX
| Spermine oxidase | 33.26 ± 7.94 | ** | 6.29 |
5977:12455_CL1Contig1:r |
TNFAIP6
| Tumor necrosis factor alpha-induced protein 6 | 2517 ± 105 | *** | 30.54 |
19103:6332_CL1Contig1:f |
TNFRSF12A
| Tumor necrosis factor receptor superfamily, member 12A | 0.52 ± 0.17 | * | 3.04 |
9402:4028_CL1Contig1:r |
UAP1
| UDP-N-acetylglucosamine pyrophosphorylase 1 | 8.01 ± 1.11 | *** | 5.71 |
FSH vs. Control - underexpressed | |||||
707:4250_CL1Contig1:f |
FOS
| Proto-oncogene protein c-fos | 8.56 ± 2.43 | * | 0.15 |
1893:12937_CL1Contig1:f |
FOXO1
| Forkhead box O1 | 0.90 ± 0.09 | ns. | 0.29 |
6206:5117_CL1Contig1:f |
FST
| Follistatin | 0.21 ± 0.02 | *** | 0.10 |
15012:1275_CL1Contig1:f |
HS3ST1
| Heparan sulfate (glucosamine) 3-O-sulfotransferase 1 | 0.80 ± 0.12 | ns. | 0.32 |
TC210305:f |
IRS1
| Insulin receptor substrate 1 | 0.98 ± 0.25 | ns. | 0.14 |
NM_213880.1 |
JUN
| Jun proto-oncogene | 1.03 ± 0.14 | ns. | 0.06 |
2480:21101_CL1Contig1:f |
PCNA
| Proliferating cell nuclear antigen | 1.16 ± 0.12 | ns. | 0.29 |
18375:27730_CL1Contig1:r |
PNRC1
| Proline-rich nuclear receptor coactivator 1 | 0.94 ± 0.15 | ns. | 0.21 |
14891:209_CL4Contig1:f |
PPARG
| Peroxisome proliferator-activated receptor gamma | 0.45 ± 0.04 | *** | 0.32 |
1456:37319_CL1Contig1:f |
PTGES
| Prostaglandin E synthase | 0.70 ± 0.10 | ** | 0.26 |
12586:12773_CL1Contig1:f |
RGS3
| Regulator of G-protein signaling 3 | 0.78 ± 0.10 | * | 0.17 |
TC229705:f |
TRIB2
| Tribbles pseudokinase 2 | 0.84 ± 0.10 | ns. | 0.33 |
AREG/EREG vs. Control - overexpressed | |||||
13062:45360_CL23Contig1:f |
ANXA1
| Annexin A1 | 0.68 ± 0.11 | * | 3.68 |
1117:45360_CL10Contig1:r |
ANXA2
| Annexin A2 | 2.51 ± 0.41 | ** | 4.41 |
45:916_CL3Contig1:f |
CTGF (CCN2) | Connective tissue growth factor | 2.20 ± 0.25 | *** | 6.19 |
6532:35657_CL1Contig1:r |
CYP11A1
| Cytochrome P450, family 11, subfamily A, polypeptide 1 | 2.68 ± 0.44 | ** | 5.82 |
14983:2713_CL1Contig1:f |
EIF4E
| Eukaryotic translation initiation factor 4E | 2.56 ± 0.72 | ns. | 4.14 |
977:13547_CL1Contig2:r |
ITPKA
| Inositol-trisphosphate 3-kinase A | 5.41 ± 1.22 | * | 3.36 |
5795:24780_CL1Contig1:r |
MARCKSL1
| MARCKS-like 1 | 4.96 ± 0.72 | *** | 3.41 |
31453:2453_233792:f |
PGR
| Progesterone receptor | 4.01 ± 0.58 | *** | 5.25 |
33911:29637_CL1Contig1:r |
SBSN
| Suprabasin | 4.33 ± 0.40 | *** | 4.34 |
TC204140:f |
SDC1
| Syndecan 1 | 1.01 ± 0.01 | ns. | 5.88 |
TC238171:f |
SERPINE1
| Serpin peptidase inhibitor, clade E, member 1 | 6.73 ± 0.65 | *** | 33.82 |
19103:6332_CL1Contig1:f |
TNFRSF12A
| Tumor necrosis factor receptor superfamily, member 12A | 4.34 ± 0.53 | ** | 7.70 |
AREG/EREG vs. Control - underexpressed | |||||
707:4250_CL1Contig1:f |
FOS
| Proto-oncogene protein c-fos | 3.29 ± 0.57 | ** | 0.09 |
1893:12937_CL1Contig1:f |
FOXO1
| Forkhead box O1 | 1.06 ± 0.25 | ns. | 0.33 |
6206:5117_CL1Contig1:f |
FST
| Follistatin | 1.01 ± 0.07 | ns. | 0.25 |
9260:5151_CL1Contig1:f |
GADD45A
| Growth arrest and DNA-damage-inducible, alpha | 1.20 ± 0.32 | ns. | 0.35 |
2134:17017_CL1Contig1:f |
ID1
| Inhibitor of DNA binding 1protein | 1.24 ± 0.16 | ns. | 0.31 |
NM_213880.1 |
JUN
| Jun proto-oncogene | 0.54 ± 0.02 | *** | 0.04 |
30832:5168_40390252:f |
NR5A2
| Nuclear receptor subfamily 5, group A, member 2 | 1.06 ± 0.08 | ns. | 0.31 |
18375:27730_CL1Contig1:r |
PNRC1
| Proline-rich nuclear receptor coactivator 1 | 0.65 ± 0.16 | * | 0.32 |
3373:854_CL1Contig1:f |
RGS2
| Regulator of G-protein signaling 2 | 1.29 ± 0.09 | ** | 0.34 |
12586:12773_CL1Contig1:f |
RGS3
| Regulator of G-protein signaling 3 | 0.36 ± 0.03 | *** | 0.13 |
TC229705:f |
TRIB2
| Tribbles pseudokinase 2 | 0.94 ± 0.11 | ns. | 0.77 |