Erschienen in:
01.12.2014 | Brief Report
Genetic characterization of porcine kobuvirus and detection of coinfecting pathogens in diarrheic pigs in Jiangsu Province, China
verfasst von:
Zhen Yang, Wenjie Jin, Zhenpeng Zhao, Weidong Lin, Di Zhang, Enqi Yu, Aijian Qin, Hanchun Yang
Erschienen in:
Archives of Virology
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Ausgabe 12/2014
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Abstract
In this study, 396 samples from diarrheic pigs on 46 pig farms in Jiangsu Province, China, were analyzed by RT-PCR. One-hundred eighty-one pigs from 37 farms tested positive for porcine kobuvirus (PKV). Phylogenetic analysis of the 3D gene from 19 isolates showed sequence homology of 88.0 %-100 % and 69.4 %-100 % for nucleotides and amino acids, respectively, while similarity to isolates of other kobuviruses was 69.6 %–78.8 % and 27.8 %–56.9 %, respectively. One-hundred eighty-five samples contained two or more pathogens, and 31/68 PKV-positive samples tested positive for other diarrheic pathogens, confirming the existence of PKV infection and coinfection.