The online version of this article (doi:10.1186/1475-2875-11-78) contains supplementary material, which is available to authorized users.
The authors have no commercial or other association that might pose a competing interest.
PKB carried out PCR amplification, DNA sequencing experiments and drafted the manuscript. MMS and PKB carried out patients' enrolments, collected clinical and epidemiological data and drafted the manuscript. YDS analysed sequencing data and drafted the manuscript. NS conceived and designed the study, monitor the field and laboratory experiments, analysed the data and drafted the manuscript. All authors read and approved the final manuscript.
Malaria continues to be a significant health problem in India. Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic. The genetic diversity of P. falciparum merozoite surface protein-1 (MSP-1) has been extensively studied from various parts of the world. However, limited data are available from India. The aim of the present study was a molecular characterization of block 2 region of MSP-1 gene from the tribal-dominated, forested region of Madhya Pradesh.
DNA sequencing analysis was carried out in 71 field isolates collected between July 2005 to November 2005 and in 98 field isolates collected from July 2009 to December 2009. Alleles identified by DNA sequencing were aligned with the strain 3D7 and polymorphism analysis was done by using Edit Sequence tool (DNASTAR).
The malaria positivity was 26% in 2005, which rose to 29% in 2009 and P. falciparum prevalence was also increased from 72% in 2005 to 81% in 2009. The overall allelic prevalence was higher in K1 (51%) followed by MAD20 (28%) and RO33 (21%) in 2005 while in 2009, RO33 was highest (40%) followed by K1 (36%) and MAD20 (24%).
The present study reports extensive genetic variations and dynamic evolution of block 2 region of MSP-1 in central India. Characterization of antigenic diversity in vaccine candidate antigens are valuable for future vaccine trials as well as understanding the population dynamics of P. falciparum parasites in this area.
Additional file 1: Figure S1. Amino acid sequence alignment of the K1 allelic types of Plasmodium falciparum msp1 gene from central India. Figure S2. Amino acid sequence alignment of the MAD20 allelic types of Plasmodium falciparum msp1 gene from central India. Figure S3. Amino acid sequence alignment of the RO33 allelic types of Plasmodium falciparum msp1 gene from central India. (DOCX 307 KB)12936_2011_2039_MOESM1_ESM.DOCX
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Jain V, Nagpal AC, Joel PK, Shukla M, Singh MP, Gupta RB, Dash AP, Mishra SK, Udhayakumar V, Stiles JK, Singh N: Burden of cerebral malaria in central India (2004-2007). AmJTrop Med Hyg. 2008, 79: 636-642.
Tetteh KK, Cavanagh DR, Corran P, Musonda R, McBride JS, Conway DJ: Extensive antigenic polymorphism within the repeat sequence of the Plasmodium falciparu merozoite surface protein 1 block 2 is incorporated in a minimal polyvalent immunogen. Infect Immun. 2005, 73: 5928-5935. 10.1128/IAI.73.9.5928-5935.2005. PubMedCentralCrossRefPubMed
Hughes AL: Positive selection and interallelic recombination at the merozoite surface antigen-1 (MSA-1) locus of Plasmodium falciparum. Mol Biol Evol. 1992, 9: 381-393. PubMed
Ariey F, Chalvet W, Hommel D, Peneau C, Hulin A, Mercereau-Puijalon O, Duchemin JB, Sarthou JL, Reynes JM, Fandeur T: Plasmodium falciparum parasites in French Guiana: limited genetic diversity and high selfing rate. AmJTrop Med Hyg. 1999, 61: 978-985.
Da Silveira LA, Dorta ML, Kimura EA, Katzin AM, Kawamoto F, Tanabe K, Ferreira MU: Allelic diversity and antibody recognition of Plasmodium falciparum merozoite surface protein 1 during hypoendemic malaria transmission in the Brazilian amazon region. Infect Immun. 1999, 67: 5906-5916. PubMedCentralPubMed
Konaté L, Zwetyenga J, Rogier C, Bischoff E, Fontenille D, Tall A, Spiegel A, Trape JF, Mercereau-Puijalon O: Variation of Plasmodium falciparum msp1 block 2 and msp2 allele prevalence and of infection complexity in two neighbouring Senegalese villages with different transmission conditions. Trans R Soc Trop Med Hyg. 1999, 93: 21-28. CrossRefPubMed
Branch OH, Takala S, Kariuki S, Nahlen BL, Kolczak M, Hawley W, Lal AA: Plasmodium falciparum genotypes, low complexity of infection, and resistance to subsequent malaria in participants in the Asembo Bay Cohort Project. Infect Immun. 2001, 69: 7783-7792. 10.1128/IAI.69.12.7783-7792.2001. PubMedCentralCrossRefPubMed
Polley SD, Tetteh KK, Cavanagh DR, Pearce RJ, Lloyd JM, Bojang KA, Okenu DM, Greenwood BM, McBride JS, Conway DJ: Repeat sequences in block 2 of Plasmodium falciparu merozoite surface protein 1 are targets of antibodies associated with protection from malaria. Infect Immun. 2003, 71: 1833-1842. 10.1128/IAI.71.4.1833-1842.2003. PubMedCentralCrossRefPubMed
Cavanagh DR, Dodoo D, Hviid L, Kurtzhals JA, Theander TG, Akanmori BD, Polley S, Conway DJ, Koram K, McBride JS: Antibodies to the N-terminal block 2 of Plasmodium falciparu merozoite surface protein 1 are associated with protection against clinical malaria. Infect Immun. 2004, 72: 6492-6502. 10.1128/IAI.72.11.6492-6502.2004. PubMedCentralCrossRefPubMed
Bharti PK, Alam MT, Boxer R, Shukla MM, Gautam SP, Sharma YD, Singh N: Therapeutic efficacy of chloroquine and sequence variation in pfcrt gene among patients with falciparum malaria in central India. Trop Med Int Health. 2010, 15: 33-40. PubMed
Singh J, Bhattacharyaji LM: Rapid staining of malarial parasites by a water soluble stain. Ind Med Gaz. 1994, 79: 102-104.
Conway DJ, Rosario V, Oduola AM, Salako LA, Greenwood BM, McBride JS: Plasmodium falciparum: intragenic recombination and nonrandom associations between polymorphic domains of the precursor to the major merozoite surface antigens. Exp Parasitol. 1991, 73: 469-480. 10.1016/0014-4894(91)90071-4. CrossRefPubMed
Mahajan RC, Farooq U, Dubey ML, Malla N: Genetic polymorphism in Plasmodium falciparu vaccine candidate antigens. Indian J Pathol Microbiol. 2005, 48: 429-438. PubMed
Ranjit MR, Sharma YD: Genetic polymorphism of falciparum malaria vaccine candidate antigen genes among field isolates in India. AmJTrop Med Hyg. 1999, 61: 103-108.
Raj DK, Das BR, Dash AP, Supakar PC: Genetic diversity in the merozoite surface protein 1 gene of Plasmodium falciparu in different malaria-endemic localities. AmJTrop Med Hyg. 2004, 71: 285-289.
Mehrizi AA, Zakeri S, Salmanian AH, Sanati MH, Djadid ND: Plasmodium falciparum: sequence analysis of the gene encoding the C-terminus region of the merozoite surface protein-1, a potential malaria vaccine antigen, in Iranian clinical isolates. Exp Parasitol. 2008, 118: 378-385. 10.1016/j.exppara.2007.10.001. CrossRefPubMed
Gómez D, Chaparro J, Rubiano C, Rojas MO, Wasserman M: Genetic diversity of Plasmodium falciparu field samples from an isolated Colombian village. AmJTrop Med Hyg. 2002, 67: 611-616.
Talisuna AO, Langi P, Bakyaita N, Egwang T, Mutabingwa TK, Watkins W, Van Marck E, D'Alessandro U: Intensity of malaria transmission, antimalarial-drug use and resistance in Uganda: what is the relationship between these three factors?. Trans R Soc Trop Med Hyg. 2002, 96: 310-317. 10.1016/S0035-9203(02)90108-2. CrossRefPubMed
Singh N, Shukla MM, Chand G, Bharti PK, Singh MP, Shukla MK, Mehra RK, Sharma RK, Dash AP: Epidemic of Plasmodium falciparu malaria in Central India, an area where chloroquine has been replaced by artemisinin-based combination therapy. Trans R Soc Trop Med Hyg. 2011, 105: 133-139. 10.1016/j.trstmh.2010.11.002. CrossRefPubMed
Paul RE, Hackford I, Brockman A, Muller-Graf C, Price R, Luxemburger C, White NJ, Nosten F, Day KP: Transmission intensity and Plasmodium falciparum diversity on the northwestern border of Thailand. AmJTrop Med Hyg. 1998, 58: 195-203.
- Genetic diversity in the block 2 region of the merozoite surface protein-1 of Plasmodium falciparum in central India
Praveen K Bharti
Man M Shukla
Yagya D Sharma
- BioMed Central
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