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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Genetic diversity in the block 2 region of the merozoite surface protein-1 of Plasmodium falciparum in central India

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Praveen K Bharti, Man M Shukla, Yagya D Sharma, Neeru Singh
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-78) contains supplementary material, which is available to authorized users.

Competing interests

The authors have no commercial or other association that might pose a competing interest.

Authors' contributions

PKB carried out PCR amplification, DNA sequencing experiments and drafted the manuscript. MMS and PKB carried out patients' enrolments, collected clinical and epidemiological data and drafted the manuscript. YDS analysed sequencing data and drafted the manuscript. NS conceived and designed the study, monitor the field and laboratory experiments, analysed the data and drafted the manuscript. All authors read and approved the final manuscript.

Abstract

Background

Malaria continues to be a significant health problem in India. Several of the intended Plasmodium falciparum vaccine candidate antigens are highly polymorphic. The genetic diversity of P. falciparum merozoite surface protein-1 (MSP-1) has been extensively studied from various parts of the world. However, limited data are available from India. The aim of the present study was a molecular characterization of block 2 region of MSP-1 gene from the tribal-dominated, forested region of Madhya Pradesh.

Methods

DNA sequencing analysis was carried out in 71 field isolates collected between July 2005 to November 2005 and in 98 field isolates collected from July 2009 to December 2009. Alleles identified by DNA sequencing were aligned with the strain 3D7 and polymorphism analysis was done by using Edit Sequence tool (DNASTAR).

Results

The malaria positivity was 26% in 2005, which rose to 29% in 2009 and P. falciparum prevalence was also increased from 72% in 2005 to 81% in 2009. The overall allelic prevalence was higher in K1 (51%) followed by MAD20 (28%) and RO33 (21%) in 2005 while in 2009, RO33 was highest (40%) followed by K1 (36%) and MAD20 (24%).

Conclusions

The present study reports extensive genetic variations and dynamic evolution of block 2 region of MSP-1 in central India. Characterization of antigenic diversity in vaccine candidate antigens are valuable for future vaccine trials as well as understanding the population dynamics of P. falciparum parasites in this area.
Zusatzmaterial
Additional file 1: Figure S1. Amino acid sequence alignment of the K1 allelic types of Plasmodium falciparum msp1 gene from central India. Figure S2. Amino acid sequence alignment of the MAD20 allelic types of Plasmodium falciparum msp1 gene from central India. Figure S3. Amino acid sequence alignment of the RO33 allelic types of Plasmodium falciparum msp1 gene from central India. (DOCX 307 KB)
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Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Authors’ original file for figure 4
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Literatur
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