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Erschienen in: Archives of Virology 3/2020

03.02.2020 | Original Article

Genetic diversity of group A rotaviruses in Moscow in 2018-2019

verfasst von: A. A. Ivashechkin, A. G. Yuzhakov, T. V. Grebennikova, K. A. Yuzhakova, N. Y. Kulikova, L. B. Kisteneva, S. V. Smetanina, M. V. Bazarova, M. G. Almazova

Erschienen in: Archives of Virology | Ausgabe 3/2020

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Abstract

Here, we present the results of a study in which 639 samples obtained between October 2018 and April 2019 from patients with symptoms of acute gastroenteritis were tested for the presence of a rotavirus infection. The antigen of group A rotavirus was detected in 160 samples (25% of those tested). To study the genetic diversity of group A rotavirus, RNA was isolated from the samples, and polymerase chain reaction combined with reverse transcription (RT-PCR) with primers specific for the VP4, VP6, and VP7 genes of group A rotaviruses was performed. At least one fragment of the group A rotavirus genome was found in 101 samples (15.8%). These fragments were sequenced, and their G and P genotypes—as well as their combinations—were determined. The predominant G genotypes were G9 (35.8% of all genotyped samples) and G4 (28.4%), but the rare G12 genotype was also found (3.0%). The dominant P genotype was P[8]. The spectrum of certain G/P combinations of genotypes included seven variants. The most common variants were G9P[8] (37.2%) and G4P[8] (30.2%).
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Literatur
2.
Zurück zum Zitat Cherepanova ЕA, Chernyavskaya OP, Morozova NS, Yasinsky AA (2012) Peculiarities of the epidemiology of rotavirus infection in the Russian Federation in 2000–2010. Epidemiol Vaccine Prev 2(63):38–41 (eLIBRARY ID: 17677507 (in Russian)) Cherepanova ЕA, Chernyavskaya OP, Morozova NS, Yasinsky AA (2012) Peculiarities of the epidemiology of rotavirus infection in the Russian Federation in 2000–2010. Epidemiol Vaccine Prev 2(63):38–41 (eLIBRARY ID: 17677507 (in Russian))
4.
Zurück zum Zitat Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy. Classification and nomenclature of viruses. Elsevier Academic Press, Oxford, pp 447–496 (ISBN 978-0-12-249951-7) Fauquet CM, Mayo MA, Maniloff J, Desselberger U, Ball LA (2005) Virus taxonomy. Classification and nomenclature of viruses. Elsevier Academic Press, Oxford, pp 447–496 (ISBN 978-0-12-249951-7)
9.
Zurück zum Zitat Estes MK, Greenberg HB (2013) Rotaviruses. In: Knipe DM, Howley PM (eds) Fields virology, 6th edn. Lippincott Williams & Wilkins, Philadelphia, p 2456 (ISBN 978-1451105636) Estes MK, Greenberg HB (2013) Rotaviruses. In: Knipe DM, Howley PM (eds) Fields virology, 6th edn. Lippincott Williams & Wilkins, Philadelphia, p 2456 (ISBN 978-1451105636)
10.
Zurück zum Zitat Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Gomara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-likeand bovine rotavirus strains. J Virol 82(7):3204–3219. https://doi.org/10.1128/JVI.02257-07 CrossRefPubMedPubMedCentral Matthijnssens J, Ciarlet M, Heiman E, Arijs I, Delbeke T, McDonald SM, Palombo EA, Iturriza-Gomara M, Maes P, Patton JT, Rahman M, Van Ranst M (2008) Full genome-based classification of rotaviruses reveals a common origin between human Wa-Like and porcine rotavirus strains and human DS-1-likeand bovine rotavirus strains. J Virol 82(7):3204–3219. https://​doi.​org/​10.​1128/​JVI.​02257-07 CrossRefPubMedPubMedCentral
11.
Zurück zum Zitat Estes MK, Kapikian AZ (2007) Rotaviruses. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE (eds) Fields virology, 5th edn. Lippincott Williams & Wilkins, Philadelphia, pp 1917–1974 (ISBN 9780781760607 0781760607) Estes MK, Kapikian AZ (2007) Rotaviruses. In: Knipe DM, Howley PM, Griffin DE, Lamb RA, Martin MA, Roizman B, Straus SE (eds) Fields virology, 5th edn. Lippincott Williams & Wilkins, Philadelphia, pp 1917–1974 (ISBN 9780781760607 0781760607)
13.
Zurück zum Zitat Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Bányai K, Estes MK, Gentsch JR, Iturriza-Gómara M, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Patton JT, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Desselberger U, Van Ranst M (2008) Recommendations for the classification of a group of rotaviruses using all 11 genomic RNA segments. Arch Virol 153(8):1621–1629. https://doi.org/10.1007/s00705-008-0155-1 CrossRefPubMedPubMedCentral Matthijnssens J, Ciarlet M, Rahman M, Attoui H, Bányai K, Estes MK, Gentsch JR, Iturriza-Gómara M, Kirkwood CD, Martella V, Mertens PP, Nakagomi O, Patton JT, Ruggeri FM, Saif LJ, Santos N, Steyer A, Taniguchi K, Desselberger U, Van Ranst M (2008) Recommendations for the classification of a group of rotaviruses using all 11 genomic RNA segments. Arch Virol 153(8):1621–1629. https://​doi.​org/​10.​1007/​s00705-008-0155-1 CrossRefPubMedPubMedCentral
15.
18.
Zurück zum Zitat Zaitseva EV, Olneva TA, Kuleshov KV, Kondratieva LM, Shikhina TM, Milikhina AV, Kalashnikova NA, Sidorova VF, Chernyshova OA, Vasilyeva NI, Yakunina OYu, Varenikova VV, Mnozhnina EG, Ivanova SA, Kaspirova LS, Fomina NS, Shaeva EI, Malokisher NS, Rud LA, Osipova SN, Chernysheva OS, Prisyazhnyuk EN, Lebedeva LA, Isaeva NV, Karavyanskaya TN, Tsarnenko OS, Podkolzin AT, Shipulin GA (2016) Results of monitoring of antigenic types of rotavirus type A on the territory of the Russian Federation in the period 2011-2015. Clin Lab Diagn 61(7):445–448. https://doi.org/10.18821/0869-2084-2016-61-7-445-448(eLIBRARY ID: 27162081 (in Russian)) CrossRef Zaitseva EV, Olneva TA, Kuleshov KV, Kondratieva LM, Shikhina TM, Milikhina AV, Kalashnikova NA, Sidorova VF, Chernyshova OA, Vasilyeva NI, Yakunina OYu, Varenikova VV, Mnozhnina EG, Ivanova SA, Kaspirova LS, Fomina NS, Shaeva EI, Malokisher NS, Rud LA, Osipova SN, Chernysheva OS, Prisyazhnyuk EN, Lebedeva LA, Isaeva NV, Karavyanskaya TN, Tsarnenko OS, Podkolzin AT, Shipulin GA (2016) Results of monitoring of antigenic types of rotavirus type A on the territory of the Russian Federation in the period 2011-2015. Clin Lab Diagn 61(7):445–448. https://​doi.​org/​10.​18821/​0869-2084-2016-61-7-445-448(eLIBRARY ID: 27162081 (in Russian)) CrossRef
24.
Zurück zum Zitat Zhirakovskaia EV, Ignatiev GM, Indikova IN, Tikunova NV (2009) Evaluating the new ELISA Kits “Rotavirus-Antigen-ELISA-BEST”. BIOprepar Prev Diagn Treat 3–4:23–28 (eLIBRARY ID: 21452631 (in Russian)) Zhirakovskaia EV, Ignatiev GM, Indikova IN, Tikunova NV (2009) Evaluating the new ELISA Kits “Rotavirus-Antigen-ELISA-BEST”. BIOprepar Prev Diagn Treat 3–4:23–28 (eLIBRARY ID: 21452631 (in Russian))
28.
Zurück zum Zitat Payne DC, Staat MA, Edwards KM, Szilagyi PG, Gentsch JR, Stockman LJ, Curns AT, Griffin M, Weinberg GA, Hall CB, Fairbrother G, Alexander J, Parashar UD (2008) Active, population-based surveillance for severely rotavirus gastroenteritis in children in the United States. Pediatrics 122(6):1235–1243. https://doi.org/10.1542/peds.2007-3378 CrossRefPubMed Payne DC, Staat MA, Edwards KM, Szilagyi PG, Gentsch JR, Stockman LJ, Curns AT, Griffin M, Weinberg GA, Hall CB, Fairbrother G, Alexander J, Parashar UD (2008) Active, population-based surveillance for severely rotavirus gastroenteritis in children in the United States. Pediatrics 122(6):1235–1243. https://​doi.​org/​10.​1542/​peds.​2007-3378 CrossRefPubMed
29.
Zurück zum Zitat Raúl Velázquez F, Calva JJ, Lourdes Guerrero M, Mass D, Glass RI, Pickering LK, Ruiz-Palacios GM (1993) Cohort study of rotavirus serotype patterns in symptomatic and asymptomatic infections in Mexican children. Pediatr Infect Dis J 12(1):54–61CrossRef Raúl Velázquez F, Calva JJ, Lourdes Guerrero M, Mass D, Glass RI, Pickering LK, Ruiz-Palacios GM (1993) Cohort study of rotavirus serotype patterns in symptomatic and asymptomatic infections in Mexican children. Pediatr Infect Dis J 12(1):54–61CrossRef
30.
Zurück zum Zitat Gorbunova MG, Zhirakovskaya EV, Tikunova NV, Stasenko VL, Vaitovich MA, Tikunov AYu, Milenina VM, Loginovsky NV (2008) The characteristic of epidemiological process of rotavirus infection in the territory in Omsk region in 1993–2007 years. Siber Med J 7(82):113–116 (eLIBRARY ID: 11684204 (in Russian)) Gorbunova MG, Zhirakovskaya EV, Tikunova NV, Stasenko VL, Vaitovich MA, Tikunov AYu, Milenina VM, Loginovsky NV (2008) The characteristic of epidemiological process of rotavirus infection in the territory in Omsk region in 1993–2007 years. Siber Med J 7(82):113–116 (eLIBRARY ID: 11684204 (in Russian))
31.
33.
Zurück zum Zitat Kilgore PE, Unicomb LE, Gentsch JR, Albert MJ, McElroy CA, Glass RI (1996) Neonatal rotavirus infection in Bangladesh: strain characterization and risk factors for nosocomial infection. Pediatr Infect Dis J 15(8):672–677CrossRefPubMed Kilgore PE, Unicomb LE, Gentsch JR, Albert MJ, McElroy CA, Glass RI (1996) Neonatal rotavirus infection in Bangladesh: strain characterization and risk factors for nosocomial infection. Pediatr Infect Dis J 15(8):672–677CrossRefPubMed
37.
Zurück zum Zitat Clark HF, Hoshino Y, Bell LM, Groff J, Hess G, Bachman P, Offit PA (1987) Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol 25(9):1757–1762CrossRefPubMedPubMedCentral Clark HF, Hoshino Y, Bell LM, Groff J, Hess G, Bachman P, Offit PA (1987) Rotavirus isolate WI61 representing a presumptive new human serotype. J Clin Microbiol 25(9):1757–1762CrossRefPubMedPubMedCentral
39.
Zurück zum Zitat Muller H, Johne R (2007) Rotaviruses: diversity and zoonotic potential—a brief review. Berl Munch Tierarztl Wochenschr 120(3–4):108–112PubMed Muller H, Johne R (2007) Rotaviruses: diversity and zoonotic potential—a brief review. Berl Munch Tierarztl Wochenschr 120(3–4):108–112PubMed
40.
Zurück zum Zitat Bakhtoyarov GN, Kiselev IS, Zverev VV, Faizuloev EB (2014) Evaluation of real-time multiplex PCR for group A rotavirus genotyping. J Microbiol Epidemiol Immunobiol 4:43–49 (eLIBRARY ID: 23929486 (in Russian)) Bakhtoyarov GN, Kiselev IS, Zverev VV, Faizuloev EB (2014) Evaluation of real-time multiplex PCR for group A rotavirus genotyping. J Microbiol Epidemiol Immunobiol 4:43–49 (eLIBRARY ID: 23929486 (in Russian))
46.
Zurück zum Zitat Martella V, Potgieter AC, Lorusso E, De Grazia S, Giammanco GM, Matthijnssens J, Bányai K, Ciarlet M, Lavazza A, Decaro N, Buonavoglia C (2011) A feline rotavirus G3P[9] carries traces of multiple reassortment events and resembles rare human G3P[9] rotaviruses. J Gen Virol 92(Pt5):1214–1221. https://doi.org/10.1099/vir.0.027425-0 CrossRefPubMed Martella V, Potgieter AC, Lorusso E, De Grazia S, Giammanco GM, Matthijnssens J, Bányai K, Ciarlet M, Lavazza A, Decaro N, Buonavoglia C (2011) A feline rotavirus G3P[9] carries traces of multiple reassortment events and resembles rare human G3P[9] rotaviruses. J Gen Virol 92(Pt5):1214–1221. https://​doi.​org/​10.​1099/​vir.​0.​027425-0 CrossRefPubMed
Metadaten
Titel
Genetic diversity of group A rotaviruses in Moscow in 2018-2019
verfasst von
A. A. Ivashechkin
A. G. Yuzhakov
T. V. Grebennikova
K. A. Yuzhakova
N. Y. Kulikova
L. B. Kisteneva
S. V. Smetanina
M. V. Bazarova
M. G. Almazova
Publikationsdatum
03.02.2020
Verlag
Springer Vienna
Erschienen in
Archives of Virology / Ausgabe 3/2020
Print ISSN: 0304-8608
Elektronische ISSN: 1432-8798
DOI
https://doi.org/10.1007/s00705-020-04534-5

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