Background
Methods
Study sample
Genotyping PCR
RFLP analysis of Pvcsp, Pvmsp 1 and Pvmsp 3α
Allele detection
Heterozygosity (HE) and mean multiplicity of infection (MOI)
Results
Allelic diversity of Pvcsp
Allele | Size | Pre-repeat | Post-repeat | n | Frequency |
---|---|---|---|---|---|
VK210a | A (650–675 bp) | Yes | Yes | 1 | 0.010 |
VK210b | A (650–675 bp) | Yes | No | 4 | 0.039 |
VK210c | A (650–675 bp) | No | No | 13 | 0.127 |
VK210d | B (675–700 bp) | Yes | Yes | 3 | 0.029 |
VK210e | B (675–700 bp) | Yes | No | 10 | 0.098 |
VK210f | B (675–700 bp) | No | Yes | 4 | 0.039 |
VK210g | B (675–700 bp) | No | No | 13 | 0.127 |
VK210h | C (701–725 bp) | Yes | Yes | 2 | 0.020 |
VK210i | C (701–725 bp) | Yes | No | 5 | 0.049 |
VK210j | C (701–725 bp) | No | Yes | 3 | 0.029 |
VK210k | C (701–725 bp) | No | No | 6 | 0.059 |
VK210l | D (725–750 bp) | No | Yes | 1 | 0.010 |
VK210m | E (750–775 bp) | Yes | No | 4 | 0.039 |
VK210n | E (750–775 bp) | No | No | 2 | 0.020 |
VK247A | A (650–675 bp) | No | ND | 4 | 0.039 |
VK247B | B (675–700 bp) | No | ND | 5 | 0.049 |
VK247C | C (701–725 bp) | No | ND | 12 | 0.118 |
VK247D | D (725–750 bp) | No | ND | 12 | 0.118 |
VK247E | E (750–775 bp) | No | ND | 3 | 0.029 |
Allelic diversity of Pvmsp 1
Allele | Size | n (frequency) | ||||
---|---|---|---|---|---|---|
Khagrachari | Cox’s Bazar | Bandarban | Rangamati | Netrokona | ||
Aa1 | A | 0 (0) | 1 (0.010) | 0 (0) | 0 (0) | 0 (0) |
Aa2 | A | 0 (0) | 2 (0.020) | 0 (0) | 0 (0) | 0 (0) |
Aa3 | A | 1 (0.010) | 5 (0.049) | 0 (0) | 0 (0) | 0 (0) |
Aa4 | A | 0 (0) | 0 (0) | 1 (0.010) | 0 (0) | 0 (0) |
Aa5 | A | 0 (0) | 3 (0.029) | 0 (0) | 0 (0) | 1 (0.010) |
Aa6 | A | 2 (0.020) | 3 (0.029) | 1 (0.010) | 0 (0) | 0 (0) |
Aa7 | A | 3 (0.029) | 7 (0.069) | 0 (0) | 0 (0) | 1 (0.010) |
Ba3 | B | 0 (0) | 1 (0.010) | 0 (0) | 0 (0) | 0 (0) |
Ba5 | B | 0 (0) | 1 (0.010) | 1 (0.010) | 0 (0) | 0 (0) |
Ba7 | B | 3 (0.029) | 33 (0.324) | 3 (0.029) | 0 (0) | 1 (0.010) |
Mixed | 4 (0.039) | 5 (0.049) | 0 (0) | 1 (0.010) | 1 (0.010) | |
Not digested | 1 (0.010) | 5 (0.049) | 3 (0.029) | 0 (0) | 0 (0) | |
Am1 | A | 0 (0) | 2 (0.020) | 0 (0) | 0 (0) | 0 (0) |
Am2 | A | 0 (0) | 0 (0) | 1 (0.010) | 0 (0) | 0 (0) |
Am3 | A | 4 (0.039) | 15 (0.147) | 1 (0.010) | 0 (0) | 2 (0.020) |
Am4 | A | 1 (0.010) | 3 (0.029) | 0 (0) | 0 (0) | 0 (0) |
Am5 | A | 0 (0) | 1(0.010) | 0 (0) | 0 (0) | 0 (0) |
Bm2 | B | 0 (0) | 1 (0.010) | 0 (0) | 0 (0) | 0 (0) |
Bm3 | B | 3 (0.029) | 34 (0.333) | 5 (0.049) | 0 (0) | 1 (0.010) |
Bm4 | B | 0 (0) | 1 (0.010) | 1 (0.010) | 0 (0) | 0 (0) |
Mixed | 4 (0.039) | 5 (0.049) | 1 (0.010) | 1 (0.010) | 1 (0.010) | |
Not digested | 2 (0.020) | 4 (0.039) | 0 (0) | 0 (0) | 0 (0) |
Allelic diversity of Pvmsp 3α
Area | |||||||
---|---|---|---|---|---|---|---|
Khagrachari | Cox’s Bazar | Bandarban | Rangamati | Netrokona | Total | Frequency | |
A (1,900 bp) | 10 | 56 | 6 | 1 | 2 | 75 | 0.735 |
B (1,400 bp) | 1 | 2 | 1 | 0 | 1 | 5 | 0.049 |
C (1,100 bp) | 2 | 5 | 2 | 0 | 0 | 9 | 0.088 |
Mixed | 2 | 10 | 0 | 0 | 1 | 13 | 0.127 |
Marker | |||
---|---|---|---|
Pvcsp
|
Pvmsp 1
|
Pvmsp 3α
| |
Number of PCR positive samples (n) | 102 | 102 | 102 |
Mean MOI | 1.07 | 1.17 | 1.13 |
HE
| 0.777 | 0.689 | 0.437 |
\({\text{P}} = \sum {\text{Pi}}^{ 2}\)
| 0.230 | 0.318 | 0.567 |
Combined probabilitya
| 0.041 |
Years |
Pvcsp
|
Pvmsp 1
|
Pvmsp 3α
| |||
---|---|---|---|---|---|---|
Mean MOI | HE
| Mean MOI | HE
| Mean MOI | HE
| |
2009 | 1.14 | 0.81 | 1.21 | 0.72 | 1.14 | 0.66 |
2010 | 1.00 | 0.66 | 1.09 | 0.69 | 1.13 | 0.31 |
2012 | 1.00 | 0.00 | 1.33 | 0.72 | 1.00 | 0.00 |
2013 | 1.00 | 0.83 | 1.25 | 0.83 | 1.00 | 0.92 |