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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Genetic polymorphism and natural selection in the C-terminal 42 kDa region of merozoite surface protein-1 among Plasmodium vivax Korean isolates

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Jung-Mi Kang, Hye-Lim Ju, Yoo-Mi Kang, Dong-Hyun Lee, Sung-Ung Moon, Woon-Mok Sohn, Jae-Won Park, Tong-Soo Kim, Byoung-Kuk Na
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-206) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

JMK, HLJ, YMK, and DHL performed all the experiments and analysed the sequence data. SUM performed sequence and phylogenetic analyses. JWP and TSK collected the blood samples. BKN and TSK designed the study and supervised the study process. BKN wrote the paper. TSK and WMS assisted in writing and editing the manuscript. All authors read and approved the final manuscript.

Abstract

Background

The carboxy-terminal 42 kDa region of Plasmodium vivax merozoite surface protein-1 (PvMSP-142) is a leading candidate antigen for blood stage vaccine development. However, this region has been observed to be highly polymorphic among filed isolates of P. vivax. Therefore it is important to analyse the existing diversity of this antigen in the field isolates of P. vivax. In this study, the genetic diversity and natural selection in PvMSP-142 among P. vivax Korean isolates were analysed.

Methods

A total of 149 P. vivax- infected blood samples collected from patients in Korea were used. The region flanking PvMSP-142 was amplified by PCR, cloned into Escherichia coli, and then sequenced. The polymorphic characteristic and natural selection of PvMSP-142 were analysed using the DNASTAR, MEGA4 and DnaSP programs.

Results

A total of 11 distinct haplotypes of PvMSP-142 with 40 amino acid changes, as compared to the reference Sal I sequence, were identified in the Korean P. vivax isolates. Most of the mutations were concentrated in the 33 kDa fragment (PvMSP-133), but a novel mutation was found in the 19 kDa fragment (PvMSP-119). PvMSP-142 of Korean isolates appeared to be under balancing selection. Recombination may also play a role in the resulting genetic diversity of PvMSP-142.

Conclusions

PvMSP-142 of Korean P. vivax isolates displayed allelic polymorphisms caused by mutation, recombination and balancing selection. These results will be useful for understanding the nature of the P. vivax population in Korea and for development of a PvMSP-142 based vaccine against P. vivax.
Zusatzmaterial
Authors’ original file for figure 1
12936_2012_2448_MOESM1_ESM.pdf
Authors’ original file for figure 2
12936_2012_2448_MOESM2_ESM.pdf
Authors’ original file for figure 3
12936_2012_2448_MOESM3_ESM.pdf
Authors’ original file for figure 4
12936_2012_2448_MOESM4_ESM.pdf
Literatur
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