The online version of this article (doi:10.1186/1475-2875-11-206) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
JMK, HLJ, YMK, and DHL performed all the experiments and analysed the sequence data. SUM performed sequence and phylogenetic analyses. JWP and TSK collected the blood samples. BKN and TSK designed the study and supervised the study process. BKN wrote the paper. TSK and WMS assisted in writing and editing the manuscript. All authors read and approved the final manuscript.
The carboxy-terminal 42 kDa region of Plasmodium vivax merozoite surface protein-1 (PvMSP-142) is a leading candidate antigen for blood stage vaccine development. However, this region has been observed to be highly polymorphic among filed isolates of P. vivax. Therefore it is important to analyse the existing diversity of this antigen in the field isolates of P. vivax. In this study, the genetic diversity and natural selection in PvMSP-142 among P. vivax Korean isolates were analysed.
A total of 149 P. vivax- infected blood samples collected from patients in Korea were used. The region flanking PvMSP-142 was amplified by PCR, cloned into Escherichia coli, and then sequenced. The polymorphic characteristic and natural selection of PvMSP-142 were analysed using the DNASTAR, MEGA4 and DnaSP programs.
A total of 11 distinct haplotypes of PvMSP-142 with 40 amino acid changes, as compared to the reference Sal I sequence, were identified in the Korean P. vivax isolates. Most of the mutations were concentrated in the 33 kDa fragment (PvMSP-133), but a novel mutation was found in the 19 kDa fragment (PvMSP-119). PvMSP-142 of Korean isolates appeared to be under balancing selection. Recombination may also play a role in the resulting genetic diversity of PvMSP-142.
PvMSP-142 of Korean P. vivax isolates displayed allelic polymorphisms caused by mutation, recombination and balancing selection. These results will be useful for understanding the nature of the P. vivax population in Korea and for development of a PvMSP-142 based vaccine against P. vivax.
Holder AA, Guevara Patino JA, Uthaipibull C, Syed SE, Ling IT, Scott-Finnigan T, Blackman MJ: Merozoite surface protein 1, immune evasion, and vaccines against asexual blood stage malaria. Parasitologia. 1999, 41: 409-414.
Holder AA: The precursor to major merozoite surface antigens: Structure and role in immunity. Prog Allergy. 1988, 41: 72-97. PubMed
Blackman MJ, Holder AA: Secondary processing of the Plasmodium falciparum merozoite surface protein-1 (MSP1) by a calcium-dependent membrane-bound serine protease: shedding of MSP-133 as a noncovalently associated complex with other fragments of the MSP1. Mol Biochem Parasitol. 1992, 50: 307-315. 10.1016/0166-6851(92)90228-C. CrossRefPubMed
Sachdeva S, Ahmad G, Malhotra P, Mukherjee P, Chauhan VS: Comparison of immunogenicities of recombinant Plasmodium vivax merozoite surface protein 1 19- and 42-kiloDalton fragments expressed in Escherichia coli. Infect Immun. 2004, 72: 5775-5782. 10.1128/IAI.72.10.5775-5782.2004. PubMedCentralCrossRefPubMed
Pitabut N, Panichakorn J, Mahakunkijcharoen Y, Hirunpetcharat C, Looareesuwan S, Khusmith S: IgG antibody profile to c-terminal region of Plasmodium vivax merozoite surface protein-1 in Thai individuals exposed to malaria. Southeast Asian J Trop Med Public Health. 2007, 38: 1-7. PubMed
Yeom JS, Kim ES, Lim KJ, Oh JH, Sohn MJ, Yoo SB, Kim E, Bae I, Jung YJ, Park JW: Naturally acquired IgM antibody response to the C-terminal region of the merozoite surface protein 1 of Plasmodium vivax in Korea: use for serodiagnosis of vivax malaria. J Parasitol. 2008, 94: 1410-1414. 10.1645/GE-1484.1. CrossRefPubMed
Zeyrek FY, Babaoglu A, Demirel S, Erdogan DD, Ak M, Korkmaz M, Coban C: Analysis of naturally acquired antibody responses to the 19-kd C-terminal region of merozoite surface protein-1 of Plasmodium vivax from individuals in Sanliurfa, Turkey. Am J Trop Med Hyg. 2008, 78: 729-732. PubMed
Pirson PJ, Perkins ME: Characterization with monoclonal antibodies of a surface antigen of Plasmodium falciparum merozoites. J Immunol. 1985, 134: 1946-1951. PubMed
Chang SP, Gibson HL, Lee NC, Barr PJ, Hui GS: A carboxyl-terminal fragment of Plasmodium falciparum gp 195 expressed by a recombinant baculovirus induces antibodies that completely inhibit parasite growth. J Immunol. 1992, 149: 548-555. PubMed
Burns JM, Parke LA, Daly TM, Cavacini LA, Weidanz WP, Long CA: A protective monoclonal antibody recognizes a variant-specific epitope in the precursor of the major merozoite surface antigen of the rodent malarial parasite Plasmodium yoelii. J Immunol. 1989, 142: 2835-2840. PubMed
Dutta S, Kaushal DC, Ware LA, Puri SK, Kaushal NA, Narula A, Upadhyaya DS, Lanar DE: Merozoite surface protein 1 of Plasmodium vivax induces a protective response against Plasmodium cynomolgi challenge in rhesus monkeys. Infect Immun. 2005, 73: 5936-5944. 10.1128/IAI.73.9.5936-5944.2005. PubMedCentralCrossRefPubMed
Galinski BR, Barnwell JW: Plasmodium vivax : who cares?. Malar J. 2008, 7: (Suppl:S9)- CrossRef
World Health Organization: Synopsis of the world Malaria situation in 1979. Wkly Epidemiol Rec. 1981, 56: 145-149.
Park JW, Klein TA, Lee HC, Pacha LA, Ryu SH, Yeom JS, Moon SH, Kim TS, Chai JY, Oh MD, Choe KW: Vivax malaria: A continuing health threat to the Republic of Korea. Am J Trop Med Hyg. 2003, 69: 159-167. PubMed
Yeom JS, Kim TS, Oh S, Sim JB, Barn JS, Kim HJ, Kim YA, Ahn SY, Shin MY, Yoo JA, Park JW: Plasmodium vivax malaria in the Republic of Korea during 2004–2005: Changing patterns of infection. Am J Trop Med Hyg. 2007, 76: 865-868. PubMed
Lim CS, Kim SH, Kwon SI, Song JW, Song KJ, Lee KN: Analysis of Plasmodium vivax merozoite surface protein-1 gene sequences from resurgent Korean isolates. Am J Trop Med Hyg. 2000, 62: 261-265. PubMed
Nei M, Gojobori T: Simple methods for estimating the numbers of synonymous and nonsynonymous nucleotide substitutions. Mol Biol Evol. 1986, 3: 418-426. PubMed
Malkin E, Long CA, Stowers AW, Zou L, Singh S, MacDonald NJ, Narum DL, Miles AP, Orcutt AC, Muratova O, Moretz SE, Zhou H, Diouf A, Fay M, Tierney E, Leese P, Mahanty S, Miller LH, Saul A, Martin LB: Phase 1 study of two merozoite surface protein 1 (MSP 142) vaccines for Plasmodium falciparum malaria. PLoS Clin Trial. 2007, 2: e12-10.1371/journal.pctr.0020012. CrossRef
Ogutu BR, Apollo OJ, McKinney D, Okoth W, Siangla J, Dubovsky F, Tucker K, Waitumbi JN, Diggs C, Wittes J, Malkin E, Leach A, Soisson LA, Milman JB, Otieno L, Holland CA, Polhemus M, Remich SA, Ockenhouse CF, Cohen J, Ballou WR, Martin SK, Angov E, Stewart VA, Lyon JA, Heppner DG, Withers MR, MSP-1 Malaria Vaccine Working Group: Blood stage malaria vaccine eliciting high antigen-specific antibody concentrations confers no protection to young children in western Kenya. PLoS One. 2009, 4: e4708-10.1371/journal.pone.0004708. PubMedCentralCrossRefPubMed
Putaporntip C, Jongwutiwes S, Sakihama N, Ferreira MU, Kho WG, Kaneko A, Kanbara H, Hattori T, Tanabe K: Mosaic organization and heterogeneity in frequency of allelic recombination of the Plasmodium vivax merozoite surface protein-1 locus. Proc Natl Acad Sci USA. 2002, 99: 16348-16353. 10.1073/pnas.252348999. PubMedCentralCrossRefPubMed
Pacheco MA, Poe AC, Collins WE, Lal AA, Tanabe K, Kariuki SK, Udhayakumar V, Escalante AA: A comparative study of the genetic diversity of the 42 kDa fragment of the merozoite surface protein-1 in Plasmodium falciparum and P. vivax. Infect Genet Evol. 2007, 7: 180-187. 10.1016/j.meegid.2006.08.002. PubMedCentralCrossRefPubMed
- Genetic polymorphism and natural selection in the C-terminal 42 kDa region of merozoite surface protein-1 among Plasmodium vivax Korean isolates
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