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01.12.2012 | Research | Ausgabe 1/2012 Open Access

Malaria Journal 1/2012

Genetic polymorphism of merozoite surface protein 2 and prevalence of K76T pfcrt mutation in Plasmodium falciparum field isolates from Congolese children with asymptomatic infections

Zeitschrift:
Malaria Journal > Ausgabe 1/2012
Autoren:
Felix Koukouikila-Koussounda, Vladimir Malonga, Pembe Issamou Mayengue, Mathieu Ndounga, Christevy Jeannhey Vouvoungui, Francine Ntoumi
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1475-2875-11-105) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors' contributions

FKK performed DNA extraction, molecular genetic studies, data analysis, and drafted the manuscript. VM and PIM participated in molecular genetic studies, CJV participated in the data analysis. MN designed and supervised the field study. FN supervised the different steps of the work. All authors contributed to the final manuscript.

Abstract

Background

In order to prepare the field site for future interventions, the prevalence of asymptomatic Plasmodium falciparum infection was evaluated in a cohort of children living in Brazzaville. Plasmodium falciparum merozoite surface protein 2 gene (msp 2) was used to characterize the genetic diversity and the multiplicity of infection. The prevalence of mutant P. falciparum chloroquine resistance transporter (pfcrt) allele in isolates was also determined.

Methods

Between April and June 2010, 313 children below 10 years of age enrolled in the cohort for malaria surveillance were screened for P. falciparum infection using microscopy and polymerase chain reaction (PCR). The children were selected on the basis of being asymptomatic. Plasmodium falciparum msp2 gene was genotyped by allele-specific nested PCR and the pfcrt K76T mutation was detected using nested PCR followed by restriction endonuclease digestion.

Results

The prevalence of asymptomatic P. falciparum infections was 8.6% and 16% by microscopy and by PCR respectively. Allele typing of the msp2 gene detected 55% and 45% of 3D7 and FC27 allelic families respectively. The overall multiplicity of infections (MOI) was 1.3. A positive correlation between parasite density and multiplicity of infection was found. The prevalence of the mutant pfcrt allele (T76) in the isolates was 92%.

Conclusion

This is the first molecular characterization of P. falciparum field isolates in Congolese children, four years after changing the malaria treatment policy from chloroquine (CQ) to artemisinin-based combination therapy (ACT). The low prevalence of asymptomatic infections and MOI is discussed in the light of similar studies conducted in Central Africa.
Zusatzmaterial
Authors’ original file for figure 1
12936_2012_2064_MOESM1_ESM.pdf
Authors’ original file for figure 2
12936_2012_2064_MOESM2_ESM.pdf
Literatur
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