Li Xia Yu and Ye Tao contributed equally to this work.
The authors declare that they have no competing interests.
LXY and YT contributed to the design of the study, the experiment conduct, data collection, data analysis and preparation of the manuscript. RMQ, YZ and QHZ contributed to the data collection and data analysis. HCL contributed to the conception and design of the study, general supervision of the research group, and critically revised the manuscript for important intellectual content. All authors read and approved the manuscript.
Streptococcus mutans (S. mutans) is the primary etiological agent of dental caries. Sortase is a transpeptidase that anchors several surface proteins to the S. mutans cell wall and has been shown to play a major role in cariogenicity. The purpose of this study was to explore the genetic polymorphisms of the sortase gene (srtA) and the social-behavioural factors associated with dental caries in children with S. mutans.
In this case–control study, 121 S. mutans strains were separately selected from caries-free children and high-severity caries children for sequencing of the srtA gene. Social and behavioural data were collected by self-administered questionnaires. Genomic DNA was extracted from S. mutans strains and amplified by PCR to obtain the srtA gene. The purified PCR products were sequenced and analysed for mutations with ABI Variant Reporter software. The distribution of missense mutations and the mean of social-behavioural factors were compared between the groups. A multiple logistic regression model was used to control for confounding factors.
The mutation frequencies at loci 168 (P = 0.023) and 470 (P = 0.032) were significantly different between the groups. The best-fitting model showed that greater age, high frequencies of solid sugar consumption, prolonged breastfeeding, a high proportion of visible plaque, and S. mutans with a T at locus 168 of the srtA gene were associated with high-severity caries in children (P < 0.05). Children carrying a G at locus 168 of S. mutans had a decreased risk for high-severity caries (OR = 0.32, 95% CI = 0.12–0.86) compared with those carrying a T.
The present study suggested that the locus 168 missense mutation of the srtA gene may correlate with caries susceptibility in children with S. mutans. In addition, age, duration of breastfeeding, solid sugar consumption, and poor oral hygiene contributed to this complex disease.
Struzycka I, Wierzbicka M, Jodkowska E, Rusyan E, Ganowicz E, Fidecki M. Oral health and prophylactic-therapeutic needs of children aged 6 years in Poland in 2012. Przegl Epidemiol. 2014;68:53–7. PubMed
Qi XQ. Report of the Third National Oral Health Survey in China (In Chinese). Beijing: People’s Medical Publishing House; 2008. p. 60–1.
Su Z. The sample size calculation and statistical analysis of case control study. In: Fang JQ, Sun ZQ, editors. Health Statistics (In Chinese). Beijing: People’s Medical Publishing House; 2008. p. 347.
Zhang R, Lin HC, Zhi QH. Caries prevalence of primary teeth and related factors in urban and rural young children of Guangzhou (In Chinese). Chin J Pract Stomatol. 2008;1:470–2.
Zhang XH, Zhou Y, Lin HC. Acquisition of Streptococcus mutans and cell adherence among 2-year old children with different caries experience (in Chinese). Chin J Pract Stomatol. 2009;2:727–9.
World Health Organization. Oral health survey, Basic Methods. 4th ed. Geneva: World Health Organization; 1997.
FDI Commission on Oral Health, Research and Epidemiology. A review of the developmental defects of enamel index (DDE index). Int Dent J. 1992;42:411–26.
Ainamo J, Bay I. Problems and proposals for recording gingivitis and plaque. Int Dent J. 1975;25:229–35. PubMed
Moye ZD, Zeng L, Burne RA. Fueling the caries process: carbohydrate metabolism and gene regulation by Streptococcus mutans. J Oral Microbiol. 2014;6:24878. CrossRef
- Genetic polymorphisms of the sortase A gene and social-behavioural factors associated with caries in children: a case–control study
Li Xia Yu
Rong Min Qiu
Qing Hui Zhi
Huan Cai Lin
- BioMed Central
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