Background
Methods
Specimens
RNA and DNA extraction
RT-PCR or PCR
Whole genome amplification
Primer name | Primer sequence (5’-3’) | Position | References |
---|---|---|---|
SAVP1F | GTGATCGTGATGGCTAATTGC | 1-21 | JX678943 |
SAVP1R | GAACTGTTTCAACACTGT | 622-639 | JX678943 |
SAVP2F | GGGACATGTGGCAGTAC | 533-549 | JX678943 |
SAVP2R | TTGAAGTAGTCCACTATCCACAT | 1087-1109 | JX678943 |
SAVP3F | ACTGACAAGTTTGCTGA | 862-878 | JX678943 |
SAVP3R | GTGTGGGGCAATTGGTGGT | 1660-1678 | JX678943 |
SAV4FW | TTGAATTGTGACCGGCCAGAG | 1600-1620 | KX688107 |
SAVP5R | TCATCATACTCATCGTCCCT | 2863-2882 | JX678943 |
SAVP6F | CTGAACACCCGTGA | 2782-2795 | JX678943 |
SAVP9R | AGCACAGCCATGGCAAAG | 4551-4568 | JX678943 |
SAVP10F | CCATAGCCACACTGTGTTCAC | 4427-4447 | JX678943 |
SAVP10R | TCTTCATCTTCATTGGTGGGAG | 5102-5123 | JX678943 |
SAVP11F | CCAAGGGCAGTGTTTGAC | 4963-4980 | JX678943 |
SAVP11R | GGTTGGTACACATAAAGTGCC | 5676-5696 | JX678943 |
SAVP12F | GATGTTAGGGCGGTGGA | 5620-5636 | JX678943 |
SAVP12R | AGGTGAAAGTGGTGTCTTCTG | 6674-6694 | JX678943 |
SAVP13F | CTCGGCACGCACACGGG | 6469-6485 | JX678943 |
SAVP13R | TGATTGGCAGGTAAATTTG | 6961-6979 | JX678943 |
SAV14N | GATGGAGTTGGCTAAAGAACA | 6893-6913 | KX688107 |
QT
| CCAGTGAGCAGAGTGACGAGGACTCGAGCTCAAGCTTTTTTTTTTTTTTTTT | [17] | |
QO
| CCAGTGAGCAGAGTGACG | [17] | |
QI
| GAGGACTCGAGCTCAAGC | [17] |
Sequence and recombination analysis
Strain ID | GenBank accession number | Length | Geographic origin | Genogroup |
---|---|---|---|---|
VZ | DQ056363 | 1940 bp | Venezuela | GIII |
YiY1/2006 | EU381231 | 1635 bp |
China
b
| GIII |
OH-MM280/2003 | AY823308 | 2971 bp | USA | GIII |
NC-QW270 | AY826426 | 2971 bp | USA | GIII |
S20 | AB242875 | 1635 bp | Japan | GIII |
HW20/2007 | HM346629 | 2983 bp | South Korea | GIII |
DG24/2007 | HM346628 | 3016 bp | South Korea | GIII |
ah-1/2009 | JX678943 | 7342 bp |
China
b
| GIII |
Cowden | AF182760 | 7320 bp | USA | GIII |
LL14 | AY425671 | 7291 bp | USA | GIII |
JJ259 | KT922089 | 7347 bp | USA | GIII |
sav1/2008 | FJ387164 | 7558 bp |
China
b
| GIII |
CH430/2012 | KF204570 | 7371 bp |
China
b
| GIII |
p2a
| KX688107 | 7371 bp |
China
b
| GIII |
WG194D-1 | KX000383 | 7496 bp | USA | GV |
TYMPo239 | AB521771 | 3949 bp | Japan | GV |
TYMPo31 | AB521772 | 3949 bp | Japan | GV |
OH-JJ674 | KJ508818 | 7198 bp | USA | GVI |
OH-JJ681 | AY974192 | 7198 bp | USA | GVI |
RV0042/2011 | KX000384 | 7150 bp | USA | GVII |
OH-LL26/2002 | AY974195 | 2952 bp | USA | GVII |
K7 | AB221130 | 7144 bp | Japan | GVII |
K10 | AB221131 | 2972 bp | Japan | GVII |
AB23 | FJ498787 | 3000 bp | Canada | GVII |
DO19/2007 | HM346630 | 2983 bp | South Korea | GVII |
2014P2 | DQ359099 | 1626 bp | Brazil | GVII |
F2-4/2006 | GU230161 | 2935 bp | Canada | GVII |
F8-9/2006 | GU230162 | 2926 bp | Canada | GVII |
WGP3/2009 | KC309420 | 2933 bp | USA | GVII |
WGP247/2009 | KC309421 | 6052 bp | USA | GVII |
WG194D/2009 | KC309416 | 6654 bp | USA | GVIII |
WG214D/2009 | KC309419 | 7497 bp | USA | GVIII |
06-18p3 | EU221477 | 3094 bp | Italy | GVIII |
F19-10 | FJ498786 | 3142 bp | Canda | GVIII |
WG180B | KC309415 | 3111 bp | USA | GVIII |
WG197C/2009 | KC309417 | 6497 bp | USA | GVIII |
WG214C | KC309418 | 3695 bp | USA | GIX |
F16-7 | FJ498788 | 2982 bp | Canada | GIX |
K8 | AB242873 | 1617 bp | Japan | GX |
2053P4 | DQ359100 | 1635 bp | Brazil | GXI |