Background
Methods
Patient samples and tissue microarray
Immunohistochemical staining and scoring system
Cell culture experiments
Protein isolation and Western blot analysis
Quantification of MG levels by HPLC
Statistical analysis
Results
Detection of AP modification and correlation with GLO1 expression in primary oropharyngeal squamous cell carcinoma
Impact of MG on GLO1 expression and viability of tumor cell lines
Impact of GLO1 inhibition on tumor cell survival
Prognostic value of the GLO1 staining pattern
GLO1high/nuc
| GLO1others
| |||||
---|---|---|---|---|---|---|
Features | Category | N | % | N | % |
p value3
|
Age [years] | < 58.25 | 18 | 64.3 | 63 | 49.2 | 0.148 |
≥ 58.25 | 10 | 35.7 | 65 | 50.8 | ||
Gender | Male | 20 | 71.4 | 96 | 75.0 | 0.695 |
Female | 8 | 28.6 | 32 | 25.0 | ||
T status | T1-T2 | 6 | 21.4 | 64 | 50.4 |
0.005
|
T3-T4 | 22 | 78.6 | 63 | 49.6 | ||
N status | N0 | 6 | 21.4 | 25 | 19.7 | 0.835 |
N+ | 22 | 78.6 | 102 | 80.3 | ||
M status | M0 | 25 | 92.6 | 120 | 96.0 | 0.444 |
M+ | 2 | 7.4 | 5 | 4.0 | ||
Pathological grading | G1–2 | 14 | 66.7 | 62 | 56.4 | 0.381 |
G3 | 7 | 33.3 | 48 | 43.6 | ||
Clinical staging | I-III | 5 | 17.9 | 44 | 34.6 | 0.084 |
IV | 23 | 82.1 | 83 | 65.4 | ||
Alcohol | no/former | 3 | 11.1 | 29 | 24.2 | 0.137 |
current | 24 | 88.9 | 91 | 75.8 | ||
Tobacco | no/former | 3 | 11.1 | 29 | 23.8 | 0.147 |
current | 24 | 88.9 | 93 | 76.2 | ||
HPV | non-related1
| 24 | 88.9 | 83 | 75.5 | 0.130 |
related2
| 3 | 11.1 | 27 | 24.5 |
Progression-free survival | Disease-specific survival | |||||
---|---|---|---|---|---|---|
Risk factor | HR | 95% CI |
p-value | HR | 95% CI |
p-value |
T status T3–4 vs T1–21
| 1.282 | 0.749–2.197 | 0.365 | 1.230 | 0.667–2.268 | 0.508 |
N status N+ vs N01
| 1.655 | 0.804–3.409 | 0.171 |
2.435
|
1.023–5.796
|
0.044
|
Clinical staging IV vs I-III1
| 1.516 | 0.771–2.983 | 0.228 | 1.702 | 0.778–3.722 | 0.183 |
Tobacco current vs never/former1
|
2.315
|
1.174–4.564
|
0.015
| 1.732 | 0.876–3.426 | 0.114 |
HPV status2
Non-related vs related1
|
0.412
|
0.200–0.849
|
0.016
|
0.356
|
0.157–0.805
|
0.013
|
Subgroup GLO1high/nuc vs GLO1others, 1
|
1.784
|
1.054–3.018
|
0.031
|
1.791
|
1.037–3.094
|
0.037
|