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01.12.2017 | Research article | Ausgabe 1/2017 Open Access

BMC Cancer 1/2017

Glyoxalase 1 expression is associated with an unfavorable prognosis of oropharyngeal squamous cell carcinoma

Zeitschrift:
BMC Cancer > Ausgabe 1/2017
Autoren:
Nele Kreycy, Christiane Gotzian, Thomas Fleming, Christa Flechtenmacher, Niels Grabe, Peter Plinkert, Jochen Hess, Karim Zaoui
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​s12885-017-3367-5) contains supplementary material, which is available to authorized users.
Abbreviations
AGEs
Advanced glycation end products
AP
Argpyrimidine
BrBzGSHCp2
S-p-bromobenzylglutathione cyclopentyl diester
DSS
Disease-specific survival
GLO1
Glyoxalase 1
HNSCC
Head and neck squamous cell carcinoma
HPV
Human papilloma virus
MG
Methylglyoxal
OPSCC
Oropharyngeal squamous cell carcinoma
PFS
Progression-free survival

Background

The Warburg effect describes a condition in which cells with high proliferative activity rely on glycolysis as a major source of energy rather than oxidative phosphorylation [ 1]. High glycolytic activity is a characteristic feature of tumor cells in pre-malignant lesions as an adaptation to intermitted hypoxia and is particularly marked in invasive tumors [ 2]. As a consequence of accelerated glycolysis cancer cells accumulate endogenous dicarbonyl compounds such as methylglyoxal (MG), a highly reactive and potent glycating agent [ 3]. Insufficient metabolism of MG causes stable modifications of proteins, e.g. modification at arginine residues known as argpyrimidine (AP), nucleotides and lipids, leading to accelerated levels of advanced glycation end products (AGEs). These modifications cause serious damage to the functional integrity of the genome and the proteome [ 4]. The carbonyl stress related to MG has been primarily described in the pathology of diabetes, where accumulation of AGEs is a common event in the manifestation and maintenance of late complications [ 5, 6]. MG is a potent cytotoxic compound and its accumulation exerts anti-tumor activity suggesting its potential use as therapeutic agent in distinct cancers [ 7]. However, several studies also reported a putative pro-tumorigenic effect of MG mainly due to post-translational modification of cancer-related proteins, indicating an impact of the global cellular context.
In mammalian cells, MG is detoxified by the glyoxalase system, an ubiquitous cellular defense mechanism comprising glyoxalase 1 (GLO1), glyoxalase 2 (GLO2/HAGH) and a catalytic amount of reduced glutathione [ 3]. The glyoxalase system has been considered to maintain tumor cell activity and viability by preventing cellular suicide due to MG accumulation. Indeed, GLO1 gene amplification and elevated expression is a common feature in the progression of multiple human malignancies, including gastric cancer [ 8], colorectal cancer [ 9], breast cancer [ 10], liver cancer [ 11, 12], skin tumors [ 13, 14], and prostate cancer [ 15]. In some tumor entities GLO1 expression was associated with advanced tumor stages or drug resistance [ 3, 8, 10, 16]. The increase in GLO1 expression and activity most likely resembles a strategy adopted by aggressive cancer cells as a defense mechanism against glycation damage induced by high intracellular MG levels as a consequence of elevated glycolytic activity or under therapeutic conditions [ 3]. Thus, GLO1 plays a vital role in tumor initiation, malignant progression as well as treatment failure, and could serve as promising target for anti-cancer therapy.
So far, neither the expression of GLO1 nor its impact on malignant progression or prognosis have been addressed for head and neck squamous cell carcinoma (HNSCC). HNSCC is one of the most common human malignancies with an annual incidence of 600,000 new cases worldwide [ 17]. Traditional risk factors are tobacco and alcohol abuse and more recently, infection by high-risk types of human papilloma virus (HPV), especially HPV16, has been related to an escalating incidence of oropharyngeal squamous cell carcinoma (OPSCC) [ 18, 19]. Despite aggressive and multimodal therapy mainly consisting of surgery, radiotherapy and platinum-based chemotherapy, the survival of patients with advanced HNSCC has only marginally improved during the past decades. Consequently, appropriate treatment of HNSCC patients is still a major challenge and there is an urgent demand for new concepts of more effective and less toxic therapy.

Methods

Patient samples and tissue microarray

Tumor specimens for this retrospective study were obtained from patients with primary OPSCC, who have given informed consent to participate and were treated at the University Hospital Heidelberg between 1990 and 2008. Formaldehyde fixed and paraffin embedded tissue specimens were provided by the tissue bank of the National Center for Tumor Disease (Institute of Pathology, University Hospital Heidelberg) after approval by the local institutional review board (ethic vote: 176/2002 and 206/2005). The study was performed according to the ethical standards of the Declaration of Helsinki. For all tumor samples, clinical and follow-up data were available from the Department of Otolaryngology, Head and Neck Surgery at the University Hospital Heidelberg and are listed in Additional file 1: Table S1. HPV16 DNA and viral transcript status was determined previously [ 20] and generation of tissue microarrays has been described elsewhere [ 21].

Immunohistochemical staining and scoring system

Tissue microarrays were assembled with independent cores from distinct areas of a formalin-fixed and paraffin embedded pre-treatment tumor tissue and for most tumors at least two independent probes were available. Tissue microarrays were incubated with anti-AP or anti-GLO1 antibodies that are listed in Additional file 2: Table S2. Immunostaining was visualized with the TSA Amplification Kit (Perkin Elmer, Rodgau, Germany) and DAB peroxidase substrate (Vector Laboratories, Burlingame, USA) according to the manufacturers instructions. Counterstaining was done by hematoxylin to visualize tissue integrity. Stained tissue microarrays were scanned using the Nanozoomer HT Scan System (Hamamatsu Photonics, Japan). Protein expression was evaluated by three independent observers using the NDP Viewer software (version 1.1.27). For AP the staining intensity (score 1 = no, score 2 = low, score 3 = moderate, score 4 = high) was evaluated. For GLO1 the relative amount of positive tumor cells (score 1 = no positive cell, score 2 ≤ 33%, 33% > score 3 ≤ 66%, score 4 > 66%) and the staining intensity (score 1 = no, score 2 = low, score 3 = moderate, score 4 = high) was evaluated (Additional file 3: Table S3). Both scores were significantly correlated (Pearson correlation coefficient 0.696, p < 0.005 and Spearman correlation coefficient 0.586, p < 0.005) and multiplied to calculate the final immunoreactivity score (IRS, range 1–16). The cut-off value for further analysis was: GLO1 high > 8 and GLO1 low ≤ 8. The intracellular staining of GLO1 was evaluated as predominant nuclear (GLO1 nuc) versus cytoplasmic (GLO1 cyt) (Additional file 4: Table S4).

Cell culture experiments

Human cell lines (FaDu and Cal27) were purchased from ATCC ( http://​www.​lgcstandards-atcc.​org; catalog numbers: HTB-43™ and CRL-2095™) and were maintained in http://​www.​sigmaaldrich.​com (Sigma, Germany) supplemented with 10% fetal bovine serum (Invitrogen, Germany), 2 mM L-Glutamine (Invitrogen, Germany) and 50 μg/ml Penicillin-Streptomycin (Invitrogen, Germany) in a humidified atmosphere of 6% CO2 at 37 °C. Authentication of both cell lines was confirmed by the Multiplex Human Cell Line Authentication Test (Multiplexion, Germany). For Western blot analysis 1 × 10 6 FaDu or Cal27 cells were seeded on 96 mm TC plates and cultured over night. Cells were treated with indicated concentrations of MG and 24 h following administration cells were harvested for protein lysates. MG was synthesized by acid hydrolysis as described [ 22], and the concentration of the stock solution was determined by derivatization with aminoguanidine [ 23]. The cell permeable GLO1 inhibitor, S- p-bromobenzylglutathione cyclopentyl diester (BrBzGSHCp 2) was prepared and characterized as described [ 24, 25]. For colony formation assays 100 and 300 FaDu cells or 300 and 1000 Cal27 cells were seeded per 6-well plate, respectively. Cells were treated with the indicated concentration of MG or the GLO1 inhibitor every second day. 10 days upon seeding cells were PFA-fixed and the amount of colonies was determined after crystal violet staining as described in [ 26]. The survival fraction was computed according to [ 27].

Protein isolation and Western blot analysis

Whole cell protein lysate was extracted using RIPA (Radioimmunoprecipitation assay) buffer [ 28] and protease and phosphatase inhibitor cocktails (Sigma-Aldrich). 20 μg of denatured protein were separated by Sodiumdodecylsulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinyl difluoride (PVDF) membranes (Millipore, Germany). After blocking with 5% milk (Roth, Germany), membranes were incubated with primary and horseradish peroxidase coupled-secondary antibodies, which are listed in Supplementary Table S2. Membranes were incubated in enhanced chemiluminiscence solution (Thermo Scientific, Germany) and developed with an E.O.S. developer (Agfa, Germany).

Quantification of MG levels by HPLC

The concentrations of MG in supernatant of cultured cells were determined by derivatization with 1,2-diamino-4,5-dimethoxybenzene and HPLC of the quinoxaline adduct by fluorescence detection as described elsewhere [ 22, 29].

Statistical analysis

Statistical analysis was conducted with the IBM SPSS Statistics software (version 21). Differences between subgroups were assessed using Chi square or Fisher’s exact tests. The method of Kaplan–Meier was used to estimate survival distributions and differences between groups were determined by log-rank tests. Univariate and multivariate Cox proportional hazard model was used to assess the association between patient subgroups and progression-free or disease-specific survival. In all statistical tests, a p-value of 0.05 or below was considered as statistically significant.

Results

Detection of AP modification and correlation with GLO1 expression in primary oropharyngeal squamous cell carcinoma

Tissue microarrays were analyzed by immunohistochemistry with an anti-AP antibody as a surrogate marker for MG accumulation in tumor cells of primary OPSCC. In parallel, GLO1 protein levels were analyzed by immunohistochemistry on serial sections of tissue microarrays. Data on AP and GLO1 immunoreactivity were available for tumor specimens of 134 patients, and revealed no AP staining in 3.7% ( n = 5), low staining pattern in 20.9% ( n = 28), moderate staining pattern in 31.4% ( n = 42), and a high staining pattern in 44% ( n = 59) (Fig. 1A, C, E and G). A heterogeneous staining pattern was also evident for GLO1 considering the staining intensity as well as the relative amount of positive tumor cells (Fig. 1B, D, F and H). It is worth noting that in addition to the expected positive staining in the cytoplasm of tumor cells (Fig. 1F), we also detected a prominent nuclear GLO1 staining in a substantial amount of OPSCC samples (Fig. 1H). Comparison of the relative GLO1 immunoreactivity score in subgroups of OPSCC patients with no, low, moderate or high AP staining demonstrated a significant positive correlation (Fig. 1I).

Impact of MG on GLO1 expression and viability of tumor cell lines

The data presented so far suggested that induced GLO1 expression is a compensatory mechanism of OPSCC tumor cells to counteract and survive the accumulation of MG. To further support this assumption FaDu and Cal27, two well-established head and neck cancer cell lines, were cultured in the presence of increasing amounts of MG. In both cell lines, administration of exogenous MG increased GLO1 protein levels as determined by Western blot analysis (Fig. 2A). It is worth noting that GLO1 levels were lower in Cal27 as compared to FaDu cells under control conditions as well as the presence of exogenous MG.
Next, the cytotoxic profile of MG treatment on tumor cell viability was analyzed by a colony-forming assay. No major difference between both cell lines was found at lower MG concentrations (≤ 40 μM, Additional file 5: Fig. S1). However, at higher MG concentrations FaDu cells displayed an improved survival as compared to Cal27 cells, which was statistical significant at a MG concentration of 160 μM ( p ≤ 0.049, Fig. 2B). These data indicated a better tolerance of tumor cells with higher basal or inducible GLO1 expression under conditions of MG accumulation.

Impact of GLO1 inhibition on tumor cell survival

To assess the consequence of GLO1 inhibition on tumor cell survival we cultured both cell lines in the presence of the cell permeable inhibitor S- p-bromobenzylglutathione cyclopentyl diester [ 3]. Quantification of MG levels in cell culture supernatant after treatment with the GLO1 inhibitor revealed a concentration dependent increase in FaDu but not Cal27 cells (Additional 6: Fig. S2). However, both cell lines showed a comparable decrease in the survival fraction with increasing amount of the GLO1 inhibitor (Fig. 2C-D). These data strongly suggested that the cytotoxic effect of GLO1 inhibition does not exclusively rely on an accumulation of MG levels.

Prognostic value of the GLO1 staining pattern

To evaluate the clinical relevance of GLO1 staining patterns in the cohort of OPSCC patients, subgroups with a low (GLO1 low, n = 67) or a high immunoreactivity score (GLO1 high, n = 89) were identified. Furthermore, we considered a cytoplasmic (GLO1 cyt, n = 94) or a predominant nuclear staining (GLO1 nuc, n = 43) in the subgroup of OPSCC patients with a positive GLO1 staining. A GLO1 high staining was significantly correlated with shorter progression-free (PFS) and disease-specific survival (DSS), while nuclear GLO1 staining revealed a trend towards shorter PFS and was significantly correlated with shorter DSS (Fig. 3A-D). These findings strongly indicated that a nuclear GLO1 accumulation in tumor cells with a high expression serves as a risk factor for unfavorable prognosis of OPSCC patients. This assumption was further assessed by a combinatorial subgroup analysis taking into account both features (GLO1 high/nuc) in comparison to all other staining patterns (GLO1 others), and confirmed a highly significant shorter PFS and DSS (Fig. 3E-F). Moreover, a high and nuclear GLO1 staining was significantly associated with a larger tumor size (Table 1), but not with any other patient characteristic tested (e.g. gender, age, TNM status, pathological grading, clinical staging, alcohol and tobacco consumption or HPV status). Next, multivariate Cox proportional hazard model analysis confirmed that a GLO1 high/nuc staining pattern served as independent risk factors for unfavorable PFS and DSS (Table 2), taking into consideration relevant prognostic risk factors based on univariate analysis (Additional 7: Table S5).
Table 1
Correlation between GLO1 high/nuc staining and patient characteristics
   
GLO1 high/nuc
GLO1 others
 
Features
Category
N
%
N
%
p value 3
 Age [years]
< 58.25
18
64.3
63
49.2
0.148
≥ 58.25
10
35.7
65
50.8
 
 Gender
Male
20
71.4
96
75.0
0.695
Female
8
28.6
32
25.0
 
 T status
T1-T2
6
21.4
64
50.4
0.005
T3-T4
22
78.6
63
49.6
 
 N status
N0
6
21.4
25
19.7
0.835
N+
22
78.6
102
80.3
 
 M status
M0
25
92.6
120
96.0
0.444
M+
2
7.4
5
4.0
 
 Pathological grading
G1–2
14
66.7
62
56.4
0.381
G3
7
33.3
48
43.6
 
 Clinical staging
I-III
5
17.9
44
34.6
0.084
IV
23
82.1
83
65.4
 
 Alcohol
no/former
3
11.1
29
24.2
0.137
current
24
88.9
91
75.8
 
 Tobacco
no/former
3
11.1
29
23.8
0.147
current
24
88.9
93
76.2
 
 HPV
non-related 1
24
88.9
83
75.5
0.130
related 2
3
11.1
27
24.5
 
1 viral DNA-negative or DNA-positive but transcript-negative; 2 viral DNA- and transcript-positive according to [ 20] ; 3 Chi-square test
Significant results ( p <0.05) (including the HR, 95% CI) are marked in boldface
Table 2
Multivariate Cox regression models for progression-free and disease-specific survival
 
Progression-free survival
Disease-specific survival
Risk factor
HR
95% CI
p-value
HR
95% CI
p-value
T status
T3–4 vs T1–2 1
1.282
0.749–2.197
0.365
1.230
0.667–2.268
0.508
N status
N+ vs N0 1
1.655
0.804–3.409
0.171
2.435
1.023–5.796
0.044
Clinical staging
IV vs I-III 1
1.516
0.771–2.983
0.228
1.702
0.778–3.722
0.183
Tobacco
current vs never/former 1
2.315
1.174–4.564
0.015
1.732
0.876–3.426
0.114
HPV status 2
Non-related vs related 1
0.412
0.200–0.849
0.016
0.356
0.157–0.805
0.013
Subgroup
GLO1 high/nuc vs GLO1 others, 1
1.784
1.054–3.018
0.031
1.791
1.037–3.094
0.037
HR Hazard ratio , CI confidence interval , 1 reference group, 2 related = viral DNA + RNA + , non-related = viral DNA + RNA or viral DNA according to [ 20]
Significant results ( p <0.05) (including the HR, 95% CI) are marked in boldface
Finally we addressed the question, whether the prognostic value of GLO1 staining patterns correlates with the mode of treatment and conducted subgroup analysis by univariate Cox regression analysis. While the prognostic value of nuclear GLO1 staining was largely independent of treatment with ( n = 109) or without surgery ( n = 46), a high GLO1 immunoreactivity score was significantly correlated with an unfavorable PFS after surgery (with or without adjuvant radio- or radiochemotherapy, Additional file 8: Fig. S3A). A similar trend was also found for DSS (Additional file 9: Fig. S3B). In contrast, a high GLO1 immunoreactivity score was significantly correlated with a poor clinical outcome in the absence of chemotherapy ( n = 108), which was not observed for patients with chemotherapy ( n = 47, Additional file 10: Fig. S4). All GLO1 staining patterns (high or nuclear GLO1 staining and their combination) were correlated with unfavorable PFS and DSS after radiotherapy ( n = 131, adjuvant and definitive), but not for surgery only ( n = 24, Additional Fig. S5). However, it is worth noting that the amount of cases without radiotherapy was limited in this retrospective cohort.

Discussion

GLO1 is a central part of a ubiquitous detoxification system in the glycolytic pathway of normal and tumor cells, and enables cell proliferation and survival under dicarbonyl stress [ 4]. Accordingly, GLO1 overexpression was found in several human malignancies [ 3, 7], and is also a common feature in tumor tissue of primary OPSCC as demonstrated by immunohistochemistry in this study. High GLO1 expression was significantly correlated with the presence of AP modifications and administration of exogenous MG induced GLO1 protein levels in two HNSCC cell lines. Induced GLO1 expression as an adaptive response to elevated MG levels was reported previously for triple negative breast cancer cell lines [ 30]. These data support a compensatory induction of GLO1 expression as a common mechanism of tumor cells to counteract the cytotoxic effect of an MG accumulation, which appears to be a critical step in the pathogenesis and malignant progression of OPSCC. Accordingly, Cal27 with lower basal and MG-induced GLO1 expression were more sensitive in a colony-forming assay to the cytotoxic effect at high MG concentrations, which is consistent with the sensitization to exogenous MG of metastatic melanoma and prostate cancer cell lines after silencing of GLO1 expression [ 14, 31].
Patients with a high GLO1 expression had a significantly shorter progression-free and disease-specific survival, suggesting a critical role of GLO1 activity not only in the initiation and maintenance of malignant tumor growth, but also the invasive capacity and metastatic spread of OPSCC tumor cells. Indeed, a significant correlation between GLO1 overexpression and tumor cell invasion, lymph node metastasis as well as reduced 5-year survival was reported for gastric cancer [ 8]. Furthermore, a growing body of experimental evidence indicates a potential role for GLO1 in tumor cell motility and invasion, which was demonstrated by ectopic GLO1 overexpression or gene silencing in established tumor cell lines derived from gastric cancer [ 8], cutaneous SCC [ 13], and prostate cancer [ 15]. In this context it is also worth noting that high GLO1 expression has been linked to the activation of distinct key regulators in oncogenic signaling, such as NF-κB, AP1 and PI3K-AKT, which might contribute to tumor cell proliferation and survival, but also accelerated tumor cell motility, metastasis and treatment resistance [ 7].
In a substantial amount of OPSCCs with GLO1 expression, we detected a predominant nuclear staining in tumor cells. Nuclear GLO1 staining was already reported for human cutaneous basal cell carcinoma [ 13] and prostate cancer [ 15], and nuclear GLO1 translocation was shown in a cell culture model of murine fibrosarcoma [ 32]. But for the first time, we demonstrate that nuclear GLO1 translocation has a clinical impact as it is significantly correlated with shorter disease-specific survival. Furthermore, the combination of high and nuclear GLO1 staining serves as independent risk factor for an unfavorable outcome of OPSCC patients. So far, the mode of regulation and causal role of nuclear GLO1 in malignant progression and treatment failure remains largely elusive and will be a major challenge for future studies. It has been speculated that one consequence of DNA damaging therapy is a dramatic increase in MG formation due to active processes of DNA repair [ 3]. As a consequence DNA and nuclear proteins become modified, which might potentiate the cytotoxic effect of anti-tumor treatment. The presence of nuclear GLO1 might serve as a potent defense mechanism to protect key regulators of DNA repair and tumor cell survival in the nucleus from inactivation by MG-induced modification.

Conclusions

In summary, the presented data support a critical role of GLO1 in the malignant progression and clinical outcome of OPSCC patients. Detection of a high and nuclear GLO1 staining pattern could be implemented in future clinical studies to identify OPSCC patients with a high risk for treatment failure, which might benefit from specific targeting of accelerated GLO1 expression and activity.

Acknowledgments

We gratefully acknowledge Leoni Erdinger, Ines Kaden, Nataly Henfling, Antje Schuhmann and Ingeborg Vogt for excellent technical assistance, Dana Holzinger for providing data on the HPV status, and Pilar Bayo and Sarika Sharma for fruitful discussion. We thank the tissue bank of the National Center for Tumor Disease (Institute of Pathology, University Hospital Heidelberg) for providing tumor specimens of OPSCC patients. We acknowledge the financial support of the German Research Foundation and Ruprecht-Karls-Universität Heidelberg within the funding program Open Access Publishing.

Funding

This work was supported in part by the German Research Foundation (HE 5760/3–1 to JH and the SFB1118 to TF) and the Helmholtz Cross Program Topic Metabolic Dysfunction (to TF). These funding included consumables for the collection, analysis, and interpretation of data, which are presented in the manuscript.

Availability of data and materials

Datasets supporting the conclusions of this article are included within the article and its additional files.

Authors’ contributions

N.K. and C.G. performed experiments and data acquisition; T.F., C.F., N.G. and P.P. provided reagents, materials and analytical tools; J.H. and K.Z. were responsible for conception and design of the study as well as data interpretation, and wrote the manuscript. All authors read and approved the final manuscript.

Competing interests

The authors declare that they have no financial conflict of interest.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Of the patients who gave informed consent to participate human tissue specimens were collected and provided by the tissue bank of the National Center for Tumor Disease (Institute of Pathology, University Hospital Heidelberg) after approval by the local institutional review board of the Medical Faculty Heidelberg (ethic votes: 176/2002 and 206/2005). The study was performed according to the ethical standards of the Declaration of Helsinki.

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