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15.02.2020 | Original Article

Grafted human chorionic stem cells restore motor function and preclude cerebellar neurodegeneration in rat model of cerebellar ataxia

verfasst von: Mohammadhosein Akhlaghpasand, Mahdi Tizro, Amir Raoofi, Arman Zeinaddini Meymand, Mohammaderfan Farhadieh, Fariba Khodagholi, Aysan Khatmi, Reza Soltani, Yadolah Hoseini, Ali Jahanian, Mahdi Eskandarian Boroujeni, Abbas Aliaghaei

Erschienen in: Metabolic Brain Disease | Ausgabe 4/2020

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Abstract

Cerebellar ataxia (CA) is a form of ataxia that adversely affects the cerebellum. Cell replacement therapy (CRT) has been considered as a potential treatment for neurological disorders. In this report, we investigated the neuro-restorative effects of human chorionic stem cells (HCSCs) transplantation on rat model of CA induced by 3-acetylpyridine (3-AP). In this regard, HCSCs were isolated and phenotypically determined. Next, a single injection of 3-AP was administered for ataxia induction, and bilateral HCSCs implantation was conducted 3 days after 3-AP injection, followed by expression analysis of a number of apoptotic, autophagic and inflammatory genes as well as vascular endothelial growth factor (VEGF) level, along with assessment of cerebellar neurodegeneration, motor coordination and muscle activity. The findings revealed that grafting of HCSCs in 3-AP model of ataxia decreased the expression levels of several inflammatory, autophagic and apoptotic genes and provoked the up-regulation of VEGF in the cerebellar region, prevented the degeneration of Purkinje cells caused by 3-AP toxicity and ameliorated motor coordination and muscle function. In conclusion, these data indicate in vivo efficacy of HCSCs in the reestablishment of motor skills and reversal of CA.

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Titel: Grafted human chorionic stem cells restore motor function and preclude cerebellar neurodegeneration in rat model of cerebellar ataxia
verfasst von: Mohammadhosein Akhlaghpasand
Mahdi Tizro
Amir Raoofi
Arman Zeinaddini Meymand
Mohammaderfan Farhadieh
Fariba Khodagholi
Aysan Khatmi
Reza Soltani
Yadolah Hoseini
Ali Jahanian
Mahdi Eskandarian Boroujeni
Abbas Aliaghaei
Publikationsdatum: 15.02.2020
Verlag: Springer US
Erschienen in: Metabolic Brain Disease / Ausgabe 4/2020
Print ISSN: 0885-7490
Elektronische ISSN: 1573-7365
DOI: https://doi.org/10.1007/s11011-020-00543-0

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Grafted human chorionic stem cells restore motor function and preclude cerebellar neurodegeneration in rat model of cerebellar ataxia

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