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24.07.2018 | Original Contribution | Ausgabe 8/2018 Open Access

European Journal of Nutrition 8/2018

Gut microbiota, dietary intakes and intestinal permeability reflected by serum zonulin in women

Zeitschrift:
European Journal of Nutrition > Ausgabe 8/2018
Autoren:
S. Mörkl, S. Lackner, A. Meinitzer, H. Mangge, M. Lehofer, B. Halwachs, G. Gorkiewicz, K. Kashofer, A. Painold, A. K. Holl, S. A. Bengesser, W. Müller, P. Holzer, S. J. Holasek
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1007/​s00394-018-1784-0) contains supplementary material, which is available to authorized users.

Abstract

Purpose

Increased gut permeability causes the trespass of antigens into the blood stream which leads to inflammation. Gut permeability reflected by serum zonulin and diversity of the gut microbiome were investigated in this cross-sectional study involving female study participants with different activity and BMI levels.

Methods

102 women were included (BMI range 13.24–46.89 kg m−2): Anorexia nervosa patients (n = 17), athletes (n = 20), normal weight (n = 25), overweight (n = 21) and obese women (n = 19). DNA was extracted from stool samples and subjected to 16S rRNA gene analysis (V1–V2). Quantitative Insights Into Microbial Ecology (QIIME) was used to analyze data. Zonulin was measured with ELISA. Nutrient intake was assessed by repeated 24-h dietary recalls. We used the median of serum zonulin concentration to divide our participants into a “high-zonulin” (> 53.64 ng/ml) and “low-zonulin” (< 53.64 ng/ml) group.

Results

The alpha-diversity (Shannon Index, Simpson Index, equitability) and beta-diversity (unweighted and weighted UniFrac distances) of the gut microbiome were not significantly different between the groups. Zonulin concentrations correlated significantly with total calorie-, protein-, carbohydrate-, sodium- and vitamin B12 intake. Linear discriminant analysis effect size (LEfSe) identified Ruminococcaceae (LDA = 4.163, p = 0.003) and Faecalibacterium (LDA = 4.151, p = 0.0002) as significantly more abundant in the low zonulin group.

Conclusion

Butyrate-producing gut bacteria such as Faecalibacteria could decrease gut permeability and lower inflammation. The diversity of the gut microbiota in women does not seem to be correlated with the serum zonulin concentration. Further interventional studies are needed to investigate gut mucosal permeability and the gut microbiome in the context of dietary factors.

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Zusatzmaterial
Supplementary Fig.1: Results of overall composition of gut microbiota with relative abundances [%] of phyla of the high (1) and the low (2) zonulin group (TIFF 619 KB)
394_2018_1784_MOESM1_ESM.tiff
Supplementary Fig. 2: Results of overall composition of gut microbiota with relative abundances [%] of phyla of the high (1) and the medium (2) and the low (3) zonulin group (TIFF 1335 KB)
394_2018_1784_MOESM2_ESM.tiff
Supplementary Fig. 3 A/B: Principal component analysis (PCoA) of the high (red) and low (blue) zonulin group. Each dot symbolizes the bacterial community composition of one individual stool sample. Axis titles indicate the percentage of the explained variation. Part A (left) shows the data for unweighted UniFrac distances, part B (right) for weighted UniFrac distances (TIFF 250 KB)
394_2018_1784_MOESM3_ESM.tiff
Supplementary Fig. 4 A/B: Principal component analysis (PCoA) of the high (red), medium (blue) and low (green) zonulin group. Each dot symbolizes the bacterial community composition of one individual stool sample. Axis titles indicate the percentage of the explained variation. Part A (left) shows the data for unweighted UniFrac distances, part B (right) for weighted UniFrac distances (TIFF 259 KB)
394_2018_1784_MOESM4_ESM.tiff
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