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02.08.2017 | Original Article

H₂S confers colonoprotection against TNBS-induced colitis by HO-1 upregulation in rats

verfasst von: Krisztina Kupai, Nikoletta Almási, Magdolna Kósa, János Nemcsók, Zsolt Murlasits, Szilvia Török, Amin Al-awar, Zoltán Baráth, Anikó Pósa, Csaba Varga

Erschienen in: Inflammopharmacology | Ausgabe 2/2018

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Abstract

Hydrogen sulfide (H₂S) is an endogenous mediator that contributes to many important physiological processes including vasodilation and vascular smooth muscle relaxation; in turn, preventing tissue damage and reducing inflammation. Heme oxygenase (HO) enzymes, of which HO-1 is inducible by harmful stimuli, were found to regulate intestinal inflammation in experimental animal models of colitis. We aimed to investigate the protective effects of H₂S against 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced colitis in rats, and whether HO enzyme system is involved in the H₂S-induced colonic cytoprotection. Male Wistar rats were treated with TNBS to induce colitis, and H₂S donor (Lawesson’s reagent) was prepared two times/day at different concentrations, and delivered per os (from day 1 to day 3). Our results suggest that daily treatment (2 times/day) with H₂S donor, could significantly decrease the extent of colonic inflammation compared to vehicle treatment, and the most effective daily dose of H₂S donor against inflammation was 18.75 µM/kg/day. Per os administration of H₂S donor increased the colonic HO enzyme activity; on the contrary, the protective effect of H₂S was abolished by the co-treatment with HO inhibitor. Our findings suggest that H₂S confers colonoprotection, probably by modulation of anti-inflammatory parameters and HO enzyme activity.

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Titel: H2S confers colonoprotection against TNBS-induced colitis by HO-1 upregulation in rats
verfasst von: Krisztina Kupai
Nikoletta Almási
Magdolna Kósa
János Nemcsók
Zsolt Murlasits
Szilvia Török
Amin Al-awar
Zoltán Baráth
Anikó Pósa
Csaba Varga
Publikationsdatum: 02.08.2017
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 2/2018
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0382-8

Springer Medizin

Abstract

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