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Erschienen in: Journal of NeuroVirology 3/2014

01.06.2014

Hepatitis C virus (HCV) interaction with astrocytes: nonproductive infection and induction of IL-18

verfasst von: Ziqing Liu, Fang Zhao, Johnny J. He

Erschienen in: Journal of NeuroVirology | Ausgabe 3/2014

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Abstract

Hepatitis C virus (HCV) infection causes the central nervous system (CNS) abnormalities in more than 50 % of chronically infected subjects. However, the underlying mechanisms are largely unknown. In this study, we characterized the HCV interactions with astrocytes, one of the putative HCV target cells in the brain. We demonstrated that primary human astrocytes (PHA) were very inefficiently infected by HCV, either in the cell-free form or through cell–cell contact. We then determined the potential restriction steps of HCV infection and replication in these cells. PHA expressed all known HCV receptors but failed to support HCV entry. HCV IRES-mediated RNA translation was functional in PHA and further enhanced by miR122 expression. Nevertheless, PHA did not support HCV replication regardless of miR122 expression. To our great surprise, we found that HCV exposure induced robust IL-18 expression in PHA and exhibited direct neurotoxicity. Taken together, these results showed that astrocytes did not support productive HCV infection and replication, but HCV interactions with astrocytes and neurons alone might be sufficient to cause CNS dysfunction.
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Metadaten
Titel
Hepatitis C virus (HCV) interaction with astrocytes: nonproductive infection and induction of IL-18
verfasst von
Ziqing Liu
Fang Zhao
Johnny J. He
Publikationsdatum
01.06.2014
Verlag
Springer US
Erschienen in
Journal of NeuroVirology / Ausgabe 3/2014
Print ISSN: 1355-0284
Elektronische ISSN: 1538-2443
DOI
https://doi.org/10.1007/s13365-014-0245-7

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