The online version of this article (doi:10.1186/1476-9255-9-16) contains supplementary material, which is available to authorized users.
The author, Hong-Ying Gao carried out the hepatoprotective effect of Matrine salvianolic acid B salt on carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats, participated in the analysis of the data and drafted the manuscript. All authors read and approved the final manuscript.
The authors declare that they have no competing interests.
The aim of this study was to investigate the hepatoprotective effect of Matrine salvianolic acid B salt on carbon tetrachloride (CCl4)-induced hepatic fibrosis in rats. Salvianolic acid B and Matrine has long been used to treat liver fibrosis. Matrine salvianolic acid B salt is a new compound containing Salvianolic acid B and Matrine. Hepatic fibrosis induced by CCl4 was studied in animal models using Wistar rats. Organ coefficient, serum aspartate aminotransferase (AST), alanine aminotransferase (ALT), hexadecenoic acid (HA), laminin (LN), hydroxyproline (Hyp), and glutathione (GSH), malondialdehyde (MDA), superoxide dismutase (SOD) in liver tissues were measured, respectively. Histopathological changes in the livers were studied by hematoxylin-eosin (H&E) staining and Masson Trichrome (MT) examination. The expression of transforming growth factor-β1 (TGF-β1) and α-smooth muscle actin (α-SMA) was observed by immunohistochemical analysis. A significant reduction in serum levels of AST, ALT, HA, LN and Hyp was observed in the Matrine salvianolic acid B salt treated groups, suggesting that the salt had hepatoprotective effects. The depletion of GSH and SOD, as well as MDA accumulation in liver tissues was suppressed by Matrine salvianolic acid B salt too. The expression of TGF-β1 and α-SMA measured by immunohistology was significantly reduced by Matrine salvianolic acid B salt in a dose-dependent manner. Matrine salvianolic acid B salt treatment attenuated the necro-inflammation and fibrogenesis induced by CCl4 injection, and thus it is promising as a therapeutic anti-fibrotic agent against hepatic fibrosis.
Additional file 1: Hepatoprotective effect of Matrine salvianolic acid B salt on Carbon Tetrachloride-Induced Hepatic Fibrosis. (DOC 33 KB)12950_2011_232_MOESM1_ESM.doc
Authors’ original file for figure 112950_2011_232_MOESM2_ESM.pdf
Authors’ original file for figure 212950_2011_232_MOESM3_ESM.pdf
Authors’ original file for figure 312950_2011_232_MOESM4_ESM.pdf
Authors’ original file for figure 412950_2011_232_MOESM5_ESM.pdf
Authors’ original file for figure 512950_2011_232_MOESM6_ESM.pdf
Albanis E, Friedman SL: Hepatic fibrosis. Pathogenesis and principles of therapy. J Clin Liver Dis. 2001, 5: 315-334. 10.1016/S1089-3261(05)70168-9. CrossRef
Ibrahim HB, Suleyman SK, Orhan KP, Mehmet Y, Ibrahim HO, Bilal U, Ahmet I: Hepatoprotective effect of Infliximab, an Anti-TNF-α Agent, on Carbon Tetrachloride-Induced Hepatic Fibrosis. Inflammation. 2008, 31: 215-221. 10.1007/s10753-008-9067-1. CrossRef
Lamireau T, Desmouliere A, Bioulac-Sage P: Mechanisms of hepatic fibrogenesis. J Arch Pediatr. 2002, 9: 392-405. 10.1016/S0929-693X(01)00800-4. CrossRef
Recknagel R, Glende EA, Dolak JA, Waller RL: Mechanisms of carbon tetrachloride toxicity. Pharma Ther. 1989, 43: 139-154. 10.1016/0163-7258(89)90050-8. CrossRef
Nadkarni GD, D’Souza NB: Hepatic antioxidant enzymes and lipid peroxidation in carbon tetrachloride-induced liver cirrhosis in rats. Biochem Med Metabol Biol. 1988, 40: 42-45. 10.1016/0885-4505(88)90102-8. CrossRef
Gassó M, Rubio M, Varela G, Cabré M, Caballería J, Alonso E, Deulofem R, Camps J, Giménez A, Pajares M, Parés A, Mato JM, Rodés J: Effects of S-adenosylmethionine on lipid peroxidation and liver fibrogenesis in carbon tetrachloride-induced cirrhosis. J Hepatol. 1996, 25: 200-205. 10.1016/S0168-8278(96)80074-2. CrossRefPubMed
Jung Y, Wan X, Yan H, Row K: Determination of matrine and oxymatrine in Sophora flavescens ait. Via high performance liquid chromatography. J Liq Chromatogr Related Technol. 2008, 31: 2752-2761. 10.1080/10826070802388326. CrossRef
Hong M, Lee J, Jung H, Jin D, Go H, Kim J, Jang B, Shin Y, Ko S: Sophora flavescens ait. on inhibits the production of pro-inflammatory cytokines through inhibition of the NF jB/IjB signal pathway in human mast cell line (HMC-1). Toxicol Vitr. 2009, 23: 251-258. 10.1016/j.tiv.2008.12.002. CrossRef
Zhang JP, Zhang M, Jin C, Zhou B, Xie WF, Guo C: Matrine inhibits production and actions of fibrogenic cytokines released by mouse peritoneal macrophages. Acta Pharmacol Sin. 2001, 22: 765-768. PubMed
Zhang J: Inhibitory Action of Salvianolic Acid B on the Contraction of Hepatic Stellate Cells Induced by Endothelin and its Mechanism. 2004, Shanghai University of Traditional Chinese Medicine, Chinese, Shanghai
Gao HY, Li GY, Wang HY, Wang JH: Structure identification of Matrine Salvianolic acid B salt. Mod Chin Med. 2010, 12: 22-25.
Li J, Niu JZ, Zeng Y: Effects of FFBJRGP on collagen deposition in hepatic Disse space in alcohol induced liver fibrosis in rat. Med J Chin PLA. 2004, 29: 567-570.
- Hepatoprotective effect of Matrine salvianolic acid B salt on Carbon Tetrachloride-Induced Hepatic Fibrosis
- BioMed Central
Neu im Fachgebiet Innere Medizin
Meistgelesene Bücher aus der Inneren Medizin
e.Med Kampagnen-Visual, Mail Icon II