Background
Methods
Collection of plant materials
Extract preparation
Chemicals
Animals
Experimental design
Blood sample collection
Determination of hepatic enzymes
Determination of Total protein
Determination of Total bilirubin
Determination of oxidative stress parameters
Superoxide dismutase (SOD) activity
Catalase activity
Reduced glutathione
Glutathione-S-transferase activity
Glutathione peroxidase activity
Determination of lipid peroxidation
Histology
Statistical analysis
Results
Effect of methanol extract of T. conophorum seeds on serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in CCl4 induced hepatotoxicity in rats after 24 h of intoxication
Effect of methanol extract of T. conophorum seeds on gamma gtutamyl transferase and Total bilirubin in CCl4 induced hepatotoxicity in rats after 24 h of intoxication
Effect of methanol extract of T. conophorum seeds on antioxidant enzyme activity in CCl4 induced hepatotoxicity in rats after 24 h of intoxication
Treatment | Parameters | ||||
---|---|---|---|---|---|
(n = 5) | CAT (Unit/mg protein) | SOD (Unit/mg protein) | GPx (Unit/mg protein) | GST (Unit/mg protein | GSH (μg/mg protein |
Group1A | 32.20 ± 1.20 | 6.00 ± 0.50 | 4.20 ± 0.22 | 0.53 ± 0.03 | 14.50 ± 0.25 |
Group IIA | ***16.42 ± 1.12a | ***2.55 ± 0.08a | ***2.69 ± 0.10a | **0.30 ± 0.05a | ***6.41 ± 0.15 |
Group IIIA | ***30.25 ± 0.50b | ***5.50 ± 0.15b | ***4.12 ± 0.11b | *0.50 ± 0.05b | ***18.20 ± 0.45 |
Group IVA | ***25.50 ± 0.55b | *3.50 ± 0.05b | **3.46 ± 0.05b | 0.35 ± 0.01a | ***15.20 ± 0.40 |
Group VA | ***28.00 ± 0.40b | ***5.06 ± 0.20b | **3.43 ± 0.02b | *0.45 ± 0.05b | ***15.00 ± 0.06 |
GroupVIA | ***23.30 ± 0.30b | 2.80 ± 0.08b | 3.13 ± 0.04b | 0.32 ± 0.03b | ***15.70 ± 0.16 |
GroupVIIA | ***27.62 ± 0.60b | **4.00 ± 0.12b | ***3.54 ± 0.09b | 0.40 ± 0.01a | ***16.20 ± 0.20 |
Effect of methanol extract of T. conophorum seeds on liver lipid peroxidation in CCl4 induced hepatotoxicity in rats after 24 h of intoxication
Effect of methanol extract of T. conophorum seeds on serum aspartate aminotransferase, alanine aminotransferase and alkaline phosphatase in CCl4 induced hepatotoxicity in rats after 48 h of intoxication
Effect of methanol extract of T. conophorum seeds on gamma gtutamyl transferase and Total bilirubin levels in CCl4 induced hepatotoxicity in rats after 48 h of intoxication
Effect of methanol extract of T. conophorum seeds on antioxidant enzyme activities in CCl4 induced hepatotoxicity in rats after 48 h of intoxication
Treatment | Parameters | ||||
---|---|---|---|---|---|
(n = 5) | Catalase (Unit/mg protein) | SOD (Unit/mg protein) | GPx (Unit/mg protein) | GST (Unit/mg protein | GSH (μg/mg protein) |
Group1B:Normal control | 70.20 ± 3.50 | 6.70 ± 0.53 | 3.80 ± 0.16 | 0.62 ± 0.02 | 16.80 ± 0.53 |
Group IIB | ***44.50 ± 1.20a | ***2.50 ± 0.50a | ***1.50 ± 0.06a | ***0.29 ± 0.001a | ***7.01 ± 0.50a |
Group IIIB | ***70.50 ± 2.50b | ***6.50 ± 0.25b | ***4.40 ± 0.15b | ***0.51 ± 0.06b | ***17.02 ± 0.25b |
Group IVB | 50.00 ± 1.17b | *4.20 ± 0.20b | ***3.46 ± 0.10b | 0.26 ± 0.01a | ***10.20 ± 0.15b |
Group VB | ***66.90 ± 2.00b | ***6.05 ± 0.15b | ***3.43 ± 0.21b | **0.43 ± 0.001b | ***15.20 ± 0.40b |
Group VIB | 48.50 ± 3.80b | 2.62 ± 0.60b | ***2.69 ± 0.07b | 0.30 ± 0.003b | ***11.80 ± 0.30b |
Group VIIB | ***68.00 ± 2.55b | ***5.50 ± 0.11b | **3.12 ± 0.20 | **0.45 ± 0.02b | ***14.00 ± 0.28b |