Letter to the editor
Cases | Consensus germline primers/probes | Patient-specific primers/probes | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
Diagnostic marrow DNA as standards | Diagnostic marrow DNA as standards | CDR3-cloned plasmid as standards | ||||||||||
Sensitivity | QR |
R
2
| Slope | Sensitivity | QR |
R
2
| Slope | Sensitivity | QR |
R
2
| Slope | |
Dx53 | N/A | 10−2
| N/A | N/A | 10−5
| 10−4
| 0.996 | −3.30 | 10−5
| 10−4
| 0.992 | −3.44 |
M3 | ND | ND | ND | ND | 5 × 10−4
| 5 × 10−4
| 0.989 | −3.48 | 10−4
| 10−4
| 0.997 | −3.54 |
M8 | 10−4
| 5 × 10−4
| 0.985 | −3.38 | 10−4
| 10−3
| 0.999 | −3.46 | 10−4
| 5 × 10−4
| 0.999 | −3.61 |
M4 | ND | ND | ND | ND | 10−4
| 10−3
| 0.984 | −3.60 | 10−5
| 10−3
| 0.999 | −3.54 |
Dx31 | ND | ND | ND | ND | 10−4
| 5 × 10−4
| 0.998 | −3.45 | 10−4
| 10−4
| 0.998 | −3.47 |
M5 | 10−4
| 10−4
| 0.995 | −3.41 | 5 × 10−4
| 5 × 10−4
| 0.995 | −3.67 | 10−5
| 10−4
| 0.998 | −3.51 |
M10 | N/A | 5 × 10−4
| N/A | N/A | N/A | 5 × 10−4
| N/A | N/A | 10−4
| 10−4
| 0.994 | −3.51 |
Dx49 | 5 × 10−4
| 10−3
| 0.982 | −3.37 | 10−4
| 10−3
| 0.986 | −3.26 | 10−4
| 5 × 10−4
| 0.999 | −3.76 |
M1 | ND | ND | ND | ND | 5 × 10−4
| 10−3
| 0.986 | −3.48 | 10−4
| 5 × 10−4
| 0.998 | −3.52 |
M6 | ND | ND | ND | ND | N/A | 5 × 10−4
| N/A | N/A | 10−5
| 5 × 10−4
| 0.997 | −3.72 |
M2 | N/A | N/A | N/A | N/A | ND | ND | ND | ND | ND | ND | ND | ND |
M7 | N/A | 10−2
| N/A | N/A | N/A | 10−2
| N/A | N/A | 10−4
| 10−3
| 0.999 | −3.56 |
M9 | N/A | 10−2
| N/A | N/A | N/A | 10−2
| N/A | N/A | 10−4
| 10−4
| 0.997 | −3.55 |
Cases | Mismatches against primers | Mismatches against probes | ||||
---|---|---|---|---|---|---|
Primers | Primer binding site sequences | No. | Probes | Probe binding site sequences | No. | |
Dx53 | R-JH6-intron | gcagaGaGGaaGggccctagagt | 4 | T-JH6 | cacggtcaccgtctcctcaggCaagaa | 1 |
M3 | R-JH4-intron | cagagAAaaTATCACagagaggttgt | 8 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
M8 | R-JH4-intron | cagagCtaaagcaggagagaggttgt | 1 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
M4 | R-JH4-intron | cagGAttTaagTaggGgagaCgttgt | 6 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
Dx31 | R-JH4-intron | TagaCCCaaagTaggagagaCAttAt | 8 | T-JH1.2.4.5 | Tcctggtcaccgtctcctcaggtg | 1 |
M5 | R-JH3-intron | aggcagaaggaaagccatcttac | 0 | T-JH3 | cCagggacaatggtcaccgtctcttca | 1 |
M10 | R-JH6-intron | gcagaaaacaaaggccctagagt | 0 | T-JH6 | cacggtcaccgtctcctcaggtaagaa | 0 |
Dx49 | R-JH6-intron | – | ? | T-JH6 | cacggtcaccgtctcctcaggtaagaT | 1 |
M1 | R-JH4-intron | – | ? | T-JH1.2.4.5 | cTGt--- | ≥2 |
M6 | R-JH3-intron | – | ? | T-JH3 | caagggacaat--- | ? |
M2 | R-JH4-intron | cagagttaaagcaggagagaggttgt | 0 | T-JH1.2.4.5 | ccctggtcGccgtctcctcaggtg | 1 |
M7 | R-JH5-intron | agagagggggtggtgaggact | 0 | T-JH1.2.4.5 | ccctggtcaccgtctcctcaggtg | 0 |
M9 | R-Jk1 | gatcacttcatagacacagggaacag | 0 | T-Jk1 | tggaaatcaaacgtgag | 0 |