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Journal of Cancer Research and Clinical Oncology

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01.07.2009 | Original Paper

High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation

verfasst von: Bing Liu, Hong Zhang, Xiaohong Luo, Yi Xie, Jifang Hao, Qingming Zhou, Xin Duan, Yanling Wang, Weiping Zhao

Erschienen in: Journal of Cancer Research and Clinical Oncology | Ausgabe 7/2009

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Abstract

Purpose

The aim of this study is to evaluate the effect of carbon-beam irradiation on adenovirus-mediated p53 transfer in human cervix adenocarcinoma.

Materials and methods

The HeLa cells pre-exposed to carbon-beam or γ-ray, were infected with replication-deficient adenovirus recombinant vectors, containing human wild-type p53 (AdCMV-p53) and green fluorescent protein (GFP) (AdCMV–GFP), respectively. The GFP transfer and p53 expression were detected by flow cytometric analysis.

Results

The GFP transfer frequency in C-beam with AdCMV-GFP groups was 38–50% more than that in γ-ray with AdCMV–GFP groups. The percentage of p53 positive cells in the C-beam with AdCMV–p53 groups was 34–55.6% more than that in γ-ray with AdCMV-p53 groups (p < 0.05), suggesting that subclinical-dose C-beam irradiation could significantly promote exogenous p53 transfer and p53 expression, and extend the duration of p53 expression in the HeLa cells. The expression of p21 increased with p53 expression in HeLa cells. The survival fractions for the 0.5–1.0 Gy C-beam with AdCMV-p53 groups were 38–43% less than those for the isodose γ-ray with AdCMV-p53 groups, and 31–40% less than those for the C-beam only groups (p < 0.05).

Conclusions

The subclinical-dose C-beam irradiation could significantly promote the transfer and expression of exogenous p53, extend the duration of p53 expression, and enhance the suppression of p53 on cervix adenocarcinoma cells.

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Titel: High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation
verfasst von: Bing Liu
Hong Zhang
Xiaohong Luo
Yi Xie
Jifang Hao
Qingming Zhou
Xin Duan
Yanling Wang
Weiping Zhao
Publikationsdatum: 01.07.2009
Verlag: Springer-Verlag
Erschienen in: Journal of Cancer Research and Clinical Oncology / Ausgabe 7/2009
Print ISSN: 0171-5216
Elektronische ISSN: 1432-1335
DOI: https://doi.org/10.1007/s00432-008-0528-6

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High-efficiency transfer and expression of AdCMV-p53 in human cervix adenocarcinoma cells induced by subclinical-dose carbon beam radiation

Abstract

Purpose

Materials and methods

Results

Conclusions

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Materials and methods

Results

Conclusions

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