Background
Micro RNAs
Aim
Methods
Clinical specimens
Healthy liver controls
Tissue collection, total RNA isolation, reverse transcription, and quantitative expression analysis
Statistical analysis
Results
Patient characteristics
No recurrence (n = 11) | Early recurrence (n = 29) | |
---|---|---|
Male (%) | 8 (73%) | 22 (76%) |
Mean age at time of resection ± STD | 69.2 ± 7.7 years | 63.0 ± 9.6 years |
Previous HCC treatment | 2 | 7 |
Liver cirrhosis (%) | 6 (55%) | 9 (31%) |
Child-Pugh A/B | 5/1 | 7/2 |
Etiology of liver disease Alcohol/viral/NASH/other | 3/2/1/5 | 4/9/4/12 |
Grade 1/2/3a
| 2/8/0 | 8/20/1 |
T 1/2/3a
| 6/4/0 | 9/8/11 |
V 0/1a
| 6/4 | 15/12 |
R 0/1a
| 10/0 | 25/3 |
Time to event | Mean surveillance 26.2 ± 5.4 months | RFS 6.2 ± 4.6 months |
Correlation of histopathological results with early HCC recurrence
No recurrence (n = 11) | Early recurrence (n = 29) |
p-value (Fisher’s test) | |
---|---|---|---|
T statusa
| T 1: 6 T 2/3: 4 | T 1: 9 T 2/3: 19 | 0.122 |
T 1/2: 10 T 3: 0 | T 1/2: 17 T 3: 11 | 0.018 | |
Gradea
| G 1: 2 G 2/3: 8 | G 1: 8 G 2/3: 21 | 0.493 |
G 1/2: 10 G 3: 0 | G 1/2: 28 G 3: 1 | 0.744 | |
Vascular invasiona
| V 0: 6 V 1: 4 | V 0: 15 V1: 12 | 0.555 |
Correlation of miRNA expression with early HCC recurrence
Hazard ratio | 95% CI |
p-value | |
---|---|---|---|
miR-135a high | 4.2 | 1.2–14.8 | 0.024 |
miR-21 high | 2.6 | 0.6–11.4 | 0.203 |
miR-135a high + R-status | 3.9 | 1.1–13.9 | 0.037 |
miR-135a high + V-status | 4.2 | 1.2–14.7 | 0.025 |
miR-135a high + liver cirrhosis | 4.2 | 1.2–14.8 | 0.024 |