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01.03.2005 | Original

High interleukin 12 and low interleukin 10 production after in vitro stimulation detected in sepsis survivors

verfasst von: Spaska A. Stanilova, Zhivko T. Karakolev, Gospodin S. Dimov, Zlatka G. Dobreva, Lyuba D. Miteva, Emil S. Slavov, Chavdar S. Stefanov, Noyko S. Stanilov

Erschienen in: Intensive Care Medicine | Ausgabe 3/2005

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Abstract

Objective

To evaluate viability of isolated peripheral blood mononuclear cells (PBMC) and production of cytokines in vitro after stimulation as prognostic factors for survival in sepsis patients.

Design

Prospective study of the biological response of PBMC in the onset of severe sepsis.

Setting

Research laboratory of molecular biology and immunology and university hospital ICU, Faculty of Medicine, Trakia University.

Patients

Twenty-three patients meeting the criteria for severe sepsis, and 14 control subjects.

Interventions

Isolated PBMC were stimulated in vitro with: C3-binding glycoprotein (C3bgp; 30 µg), lipopolysaccharide (30 µg), phytohemagglutinin (20 µg), pokeweed mitogen (30 µg), and dexamethasone (500 µg).

Measurements and results

We measured the levels of interleukins (IL) 6, 10, and 12 in culture supernatants. Stimulation with C3bgp and phytohemagglutinin led to significantly lower PBMC secretion of IL-6 in nonsurvivors than in survivors and healthy donors. Stimulation with C3bgp, lipopolysaccharide, and pokeweed mitogen considerably reduced IL-12 production in nonsurvivors. Stimulation with lipopolysaccharide and pokeweed mitogen caused immune cells in nonsurvivors to produce higher levels of IL-10 than in survivors. Survival of PBMC reduced viability for nonsurvivors’ PBMC, both spontaneously and as induced by lipopolysaccharide or pokeweed mitogen.

Conclusions

The viability of PBMC at the onset of sepsis and enhanced production of IL-12 and diminished production of IL-10 after stimulation with all stimuli used may be a favorable prognostic factor in sepsis.

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Titel: High interleukin 12 and low interleukin 10 production after in vitro stimulation detected in sepsis survivors
verfasst von: Spaska A. Stanilova
Zhivko T. Karakolev
Gospodin S. Dimov
Zlatka G. Dobreva
Lyuba D. Miteva
Emil S. Slavov
Chavdar S. Stefanov
Noyko S. Stanilov
Publikationsdatum: 01.03.2005
Verlag: Springer-Verlag
Erschienen in: Intensive Care Medicine / Ausgabe 3/2005
Print ISSN: 0342-4642
Elektronische ISSN: 1432-1238
DOI: https://doi.org/10.1007/s00134-005-2575-7

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Measurements and results

Conclusions

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Abstract

Objective

Design

Setting

Patients

Interventions

Measurements and results

Conclusions

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