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Neuroscience Bulletin
Erschienen in:

01.06.2019 | METHOD

High-Throughput Automatic Training System for Spatial Working Memory in Free-Moving Mice

verfasst von: Shimin Zou, Chengyu Tony Li

Erschienen in: Neuroscience Bulletin | Ausgabe 3/2019

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Abstract

Efficient behavioral assays are crucial for understanding the neural mechanisms of cognitive functions. Here, we designed a high-throughput automatic training system for spatial cognition (HASS) for free-moving mice. Mice were trained to return to the home arm and remain there during a delay period. Software was designed to enable automatic training in all its phases, including habituation, shaping, and learning. Using this system, we trained mice to successfully perform a spatially delayed nonmatch to sample task, which tested spatial cognition, working memory, and decision making. Performance depended on the delay duration, which is a hallmark of working memory tasks. The HASS enabled a human operator to train more than six mice simultaneously with minimal intervention, therefore greatly enhancing experimental efficiency and minimizing stress to the mice. Combined with the optogenetic method and neurophysiological techniques, the HASS will be useful in deciphering the neural circuitry underlying spatial cognition.
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Metadaten
Titel
High-Throughput Automatic Training System for Spatial Working Memory in Free-Moving Mice
verfasst von
Shimin Zou
Chengyu Tony Li
Publikationsdatum
01.06.2019
Verlag
Springer Singapore
Erschienen in
Neuroscience Bulletin / Ausgabe 3/2019
Print ISSN: 1673-7067
Elektronische ISSN: 1995-8218
DOI
https://doi.org/10.1007/s12264-019-00370-z

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