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01.10.2010 | Translational Research and Biomarkers | Ausgabe 10/2010

Annals of Surgical Oncology 10/2010

Higher Expression of the Heterogeneous Nuclear Ribonucleoprotein K in Melanoma

Zeitschrift:
Annals of Surgical Oncology > Ausgabe 10/2010
Autoren:
PhD Fushi Wen, BS Alex Shen, BS Reneé Shanas, MD Achyut Bhattacharyya, MD Fangru Lian, MD Galen Hostetter, MD, PhD Jiaqi Shi
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1245/​s10434-010-1121-1) contains supplementary material, which is available to authorized users.

Abstract

Background

The heterogeneous nuclear ribonucleoprotein (hnRNP) K is an essential RNA and DNA binding protein involved in gene expression and signal transduction. The role of hnRNP K in cancer is relatively understudied. However, several cellular functions strongly indicate that hnRNP K is involved in tumorigenesis. Oncogenes c-Src, c-myc, and eIF4E are regulated by hnRNP K. We have shown an increased cytoplasmic hnRNP K in pancreatic cancer. In the present study, we investigated the altered expression of hnRNP K protein and its correlation with p-ERK in melanoma using human melanoma cell lines and tissue microarray.

Materials and Methods

The protein levels of hnRNP K and p-ERK in 8 human melanoma cell lines and a melanoma progression tissue microarray containing 80 melanoma, 23 dysplastic nevi, and 14 benign nevi specimens were analyzed using Western blot and immunohistochemistry analysis. hnRNP K was knocked down by siRNA, and its effect on melanoma cells was assessed.

Results

We showed a higher hnRNP K protein level in both melanoma cell lines and melanoma tissue specimens, which correlated with a higher c-myc expression. An increase in the cytoplasmic hnRNP K and eIF4E protein levels in melanoma cells is also seen. p-ERK level was also higher in dysplastic nevi and melanoma tissues, but did not correlate with hnRNP K protein level. We then demonstrated that knocking down of hnRNP K by siRNA inhibited melanoma cell growth and colony formation, as well as c-myc expression.

Conclusions

hnRNP K expression correlated with melanoma and may play a role in melanoma tumorigenesis.

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Zusatzmaterial
Supplementary material 1 (DOC 411 kb)
10434_2010_1121_MOESM1_ESM.doc
Supplementary material 2 (TIFF 58 kb)
10434_2010_1121_MOESM2_ESM.tif
Literatur
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