Background
Glaucoma is the leading cause of permanent vision loss and irreversible blindness in the world [
1]. Acute glaucoma is caused by a blockage around the trabecular meshwork allowing buildup of aqueous humor which results in a rapid increase in intraocular pressure (IOP). This causes retinal ischemic reperfusion (IR) injury and retinal ganglion cell (RGC) death [
2]. The precise mechanisms by which elevated IOP leads to RGC death are unclear absolutely. High-mobility group box 1 (HMGB1) protein is an abundant protein that has been shown to be involved in the pathogenesis of several inflammatory diseases, including elevated IOP-induced of the inherited glaucoma rat model [
3‐
5]. Once released by necrotic cells in the extracellular milieu, HMGB1 functions as an alarming or a damage-associated molecular pattern (DAMP) [
6,
7]. Several reports have demonstrated that HMGB1 mediates ischemia-associated inflammatory responses, inducing damage in several IR diseases [
8‐
11]. Extracellular HMGB1 induces IR inflammatory responses by directly acting on pattern recognition receptors, including Toll-like receptors (TLR) 2 and 4 and receptors for advanced glycation end products (RAGEs) [
12‐
14].
Inflammasomes are intracellular multi-protein cytoplasmic complexes that play a central role in several IR diseases [
15‐
18]. Canonical inflammasomes typically are multi-protein assemblies formed by the adaptor protein apoptosis-associated speck-like protein containing a CARD (ASC), effector caspase-1, and NOD-like receptor (NLR) family or absent in melanoma 2 (AIM2) [
19,
20]. In IR diseases, DAMPs trigger the activation of NLRs which oligomerize to form a platform for the inflammasome, leading to processing of pro IL-1β into its mature forms via caspase-1 activity [
21‐
23]. Previous reports have shown nucleotide-binding domain, leucine-rich repeat containing protein 3 (NLRP3) inflammasome to be involved in IR injury by promoting the release of HMGB1 [
24,
25]. However, the contribution of HMGB1 to NLRP3 inflammasome activation has not been explored in IR injury. In order to investigate the effect of HMGB1 on the activation of NLRP3 inflammasome in acute glaucoma, we injected exogenous recombinant (r) HMGB1 and anti-HMGB1 antibody into vitreous in our acute glaucoma model. We hypothesized that HMGB1 would promote the activation of NLRP3 inflammasome to mediate retinal ischemic damage and RGC death.
Caspase-8, an initiator caspase with a critical role in triggering cell apoptosis, is synthesized as a pro-enzyme and contains a large N-terminal prodomain and a C-terminal catalytic domain composed of a large and small subunit separated by a small linker [
26]. Our previous study showed that elevated IOP could induce the activation of TLR4-caspase-8 pathway which led to retinal ischemic damage and RGC death [
27]. Since HMGB1 is the endogenous ligand of TLR4, we therefore hypothesized that HMGB1 may be involved in acute glaucoma via regulating caspase-8 activation and the process of IL-1β. Greater understanding of HMGB1 in acute glaucoma may lead to new therapeutic targets that may halt the retinal ischemic damage and RGC death.
Methods
Ethics statement
C57BL/6 female mice of 6–8 weeks of age were purchased from the Animal Research Center at Zhongshan University. The care and use of animals adhered to the Association for Research in Vision and Ophthalmology (ARVO) Statement for the use of Animals in Ophthalmic and Vision Research and were approved and monitored by the Institute of Committee of Animal Care of the Zhongshan Ophthalmic Center (Permit Number: 2011–038).
Establishment of retinal IR model
Mice were anesthetized with 10 ml/kg of 4.3 % chloral hydrate by intraperitoneal injection. Pupils were dilated with 1 % tropicamide and corneas were anesthetized topically with 0.5 % tetracaine hydrochloride eye drops. Cannulation of the anterior chamber of the right eye with a 30-gauge needle was performed to increase and maintain IOP at 70 mmHg by addition of Balance Salt Solution (Tono-Pen XL; Medtronic Solan, Jacksonville, FL, USA) for 60 min. The left eye served as a control. After 60 min, the needle was withdrawn and tobramycin was applied to avoid bacterial infection. Mice were sacrificed at 6, 24, 48, or 72 h after the procedure.
Intravitreal injection
After induction of IR injury, mice received an intravitreal injection of recombinant human HMGB1 (1 μg/2 μl; 1690-HM-025, R&D system, Inc., USA), glycyrrhizic acid (120 μM/2 μl; 50531, Sigma Aldrich, USA), nuclear factor κB (NF-κB) inhibitor JSH-23 (20 μM/2 μl; J4455, Sigma Aldrich, USA) , caspase-8 inhibitor Z-IETD-fmk (20 μM/2 μl; Calbiochem, San Diego, CA, USA), and PBS (2 μl) vehicle as sham. Mice were sacrificed and eyes were enucleated 48 h after intravitreal injection.
Histological examination
At the experimental time points, mice were sacrificed and eyes were enucleated and fixed with 4 % paraformaldehyde overnight prior to paraffin embedding. Four 4-mm-thick sections through the optic nerve of each eye were cut and stained with hematoxylin and eosin (HE). Total retinal thickness (from inner to outer limiting membrane, ILM-OLM) was measured in four adjacent areas within 1 mm distance to the optic nerve center using Axiovision software (Carl Zeiss MicroImaging Inc., Thornwood, NY, USA). The samples for confocal immunofluorescent staining were embedded in an optimal cutting temperature compound (OCT) (Tissue-Tek, Sakura Finetek USA, Torrance, CA, USA) and stored at −80 °C for frozen sectioning. The section was made in 6 μm, blocked and permeated with 5 % BSA-0.5 % Triton for 1 h at room temperature (RT), and incubated with primary antibody to detect cleaved-caspase-8 (1:500; #8592, Cell Signaling Technology, Beverly, MA, USA) at 4 °C overnight. Alexa Fluor 488 donkey anti-rabbit IgG (A-21206, Invitrogen, Carlsbad, CA, USA; 1:400) was used to visualize the primary antibody. Nuclei were stained with 4′, 6-diamidino-2-phenylindole dihydrochloride (D1306, DAPI, Invitrogen, Carlsbad, CA, USA). 400× images were collected and analyzed with a confocal microscope (Carl Zeiss, Inc, Germany). Statistical analysis was used to compare the differences among sham, high-mobility group box 1 (rHMGB1), glycyrrhizic acid (GA), IR + PBS, IR+ rHMGB1 and IR + GA groups.
Retinal flat and RGCs quantification
Retinal flat mounts from each group were fixed with 4 % paraformaldehyde for 15 min at RT, rinsed with PBS three times and blocked and permeated with 20 % BSA-0.5 % Triton-X 100 at RT for 2 h. To detect RGC markers, flat mounts were incubated overnight at 4 °C with 1:200 anti-β3-tubulin primary antibody (#5568, poly rabbit anti β3-tubulin, Cell Signaling Technology, Beverly, MA, USA). Secondary antibody was used as frozen section for confocal microscope. Images were collected and analyzed with a fluorescent microscope (Carl Zeiss, Inc, Germany). Four 400× images were obtained from each of the four quadrants of whole retinal flat mounts. The intensity of fluorescence was analyzed by using Image Pro Plus (Version 6.0; Media Cybernetics).
Semi-quantitative reverse transcription-polymerase chain reaction
Total RNA was extracted from retina samples using Trizol Reagent (Invitrogen, Carlsbad, CA, USA). cDNA was synthesized with PrimeScript RT Master Mix (DRR036A, TaKaRa, Dalian, China). PCR was carried out using a Premix EX Taq Kit (D335A, TaKaRa, Dalian, China) for 25 cycles of GAPDH and 28–30 cycles of the other target genes. PCR products were run on a 1.5 % agarose gel, and gene expression was evaluated by relative pixel densitometry using Image J software (National Institutes of Health, USA) after normalization to GAPDH. The primer sequences are as follows: caspase-8 (forward, 5-ctccgaaaaatgaaggacaga-3; reverse, 5-cgtgggataggatacagcaga-3), nlrp3 (forward, 5-ggtcctctttaccatgtgcttc-3; reverse, 5-aagtcatgtggctgaagctgta-3), asc (forward, 5-cttgtcaggggatgaactcaa-3; reverse: 5-ctggtccacaaagtgtcctgt-3), il-1beta (forward, 5-tgaaatgccaccttttgacag-3; reverse, 5-ccacagccacaatgagtgatac-3), gapdh (forward, 5-aggtcatcccagagctgaacg-3; reverse: 5-caccctgttgctgtagccgtat-3).
Western blot analysis
Total and cytoplasmic protein was isolated from retina samples. Proteins were run on 10 or 12 % polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes. PVDF membranes were blocked with 5 % BSA at RT for 60–90 min and incubated overnight at 4 °C with antigen-specific primary antibodies. Blots were then incubated with species-specific HRP-conjugated secondary antibodies for 60 min at RT. Proteins were visualized by incubation with a chemiluminescence substrate kit (ECL Plus; Perkin Elmer Inc., Covina, CA, USA). The expression of target proteins was quantified by Quantity One software (The Discovery Series) after normalizing to β-actin or GAPDH.
The primary antibodies and dilutions were used as follows: NLRP3 (1:500; NBP1-77080, Novus, Littleton, CO, USA, 100 kD), ASC (1:500; 04–147, Millipore, Temecula, CA, USA, 22 kD), phosphor-NF-κB p65 (1:1000; #3033, Cell Signaling Technology, Beverly, MA, USA, 65 kD), cleaved caspase-8 (1:500; #8592, Cell Signaling Technology, Beverly, MA, USA, 18 kD), caspase-1 (1:200; AB1871, Chemicon, International, Inc., USA, pro-caspase-1 45 kD, cleaved-caspase-1 20 kD), IL-1β (1:500; #8689, Cell Signaling Technology, Beverly, MA, USA, pro-IL-1β 31 kD, IL-1β 17 kD), β-actin (1:1000; MAB1445, MultiSciences Biotech, Hangzhou, China, 45 kD), and GAPDH (#2118, Cell Signaling, Boston, MA, USA, 36 kD).
Immunoprecipitation
Total protein was extracted from each group as stated above and stored at −80 °C. Incubated with 1 μl anti-caspase-8 or ASC antibody overnight at 4 °C was 50 μl of protein. This reaction mixture was then incubated with protein A magnetic beads (2366538, Millipore, Temecula, CA, USA) for 30 min at 4 °C. Precipitates were washed three times with washing buffer and then eluted from protein A magnetic beads by boiling with 1 × SDS for 10 min at 90–100 °C. Western blot analysis was used to evaluate the expression of caspase-8 and ASC. Immunoprecipitation antibody, anti-caspase-8 (#8592, Cell Signaling Technology, Beverly, MA), anti-ASC (sc-22513,Santa Cruz, CA, USA); Western blot analysis anti-caspase-8 (sc-6134, Santa Cruz, CA, USA), anti-ASC (ab175449, Abcam), homophytic IgG as the negative control.
Statistical analysis
The data were presented as mean ± SD or percentage. One-way ANOVA, followed by the Dunnett’s multiple comparison tests and two-way ANOVA were performed using GraphPad Prism software (version 5.0, GraphPad Software, Inc., San Diego, CA, USA). All statistical assessments were two-sided, and P values less than 0.05 were considered statistically significant.
Discussion
Acute glaucoma is a significantly sight-threatening cause of irreversible blindness in the world characterized by a sudden and substantial IOP increase and subsequent RGC death [
32,
33]. Acute elevated IOP induced retinal ischemic inflammatory injury, resulting in RGC death. We previously demonstrated that TLR4 promoted the activation of NLRP3 inflammasome which involves in the inflammatory injury of retinal ischemia [
27,
34]. However, the role of endogenous ligand of TLR4, HMGB1, is not completely understood. Several studies have reported that NLRP3 promotes the release of HMGB1 and IL-1β [
35,
36]. The object of this study is to further explore the influence of HMGB1 on inflammasome activation.
Several reports have demonstrated that HMGB1 plays a critical role in ischemic diseases [
8‐
11]. Likewise, we demonstrated that retinal IR injury triggered the HMGB1 release in retina, accompanying with retinal damage and RGC death. Increase of HMGB1 in retinal IR injury is consistent with the study reported by Dvoriantchikova and Yang [
21,
37]. It is well-known that HMGB1 is an endogenous ligand of TLR4. The HMGB1-TLR4 pathway mediates certain ischemic diseases [
38,
39]. We also previously demonstrated that TLR4 promotes the retinal ischemic damage via activating caspase-8 signaling and NLRP1 and NLRP3 inflammasomes and processing IL-1β maturation [
27]. This prompted us to further explore the mechanism of HMGB1 in mediating retinal IR injury. We injected exogenous HMGB1 or HMGB1 inhibitor into vitreous of retinal IR mice to investigate the biological function of HMGB1 in retinal IR injury. We found that HMGB1 significantly promoted cleaved caspase-8 up-regulation and canonical NLRP3-inflammasome activation, induced the processing of IL-1β, and resulted retinal injury and RGC death eventually.
Traditionally, caspase-8 has been primarily viewed as an initiator of apoptotic cell death activated by receptors of the TNF/NGF family [
40‐
42]. Recently, multiple non-apoptotic roles of caspase-8 have been reported. Importantly, caspase-8 has been reported to have a neuro-inflammatory role [
42]. In our previous study, we demonstrated that activation of caspase-8 was involved in the inflammation of ischemic retinal damage via promoting microglia activation, NLRP1/NLRP3 inflammasome activation, processing of IL-1β, and RGC death [
27]. In the present study, we firstly provided additional evidence that caspase-8 assembled a novel caspase-8-ASC inflammasome in retinal IR model. Our observation showed that non-cannonical caspase-8 inflammasome was significantly activated in ischemic retinal tissue, which could also been newly found in fungal infection [
30,
31]. And the formation of novel caspase-8-ASC inflammasome was relative to the increased HMGB1. Therefore, our data showed that HMGB1 induced the processing of IL-1β via both NLRP3-inducing caspase-1 pathway and non-caspase-1 dependent caspase-8 pathway. Overall, these results indicated that HMGB1 contributed to the inflammation of retinal ischemic damage by promoting the activation of NLRP3 inflammasome, the novel caspase-8 inflammasome, and the processing of IL-1β mediating retinal ischemic damage.
The downstream signaling of all TLR receptors involves three major signaling pathways: mitogen-activated protein kinases (MAPKs), interferon regulatory factors (IRFs), and NF-κB [
43]. It has been demonstrated that NF-κB signaling pathway activation was involved in the production of IL-1β in tissue ischemic damage [
44]. In the present study, we sought to determine the molecular mechanism of HMGB1 on the processing of IL-1β. Our findings showed that phosphor-NF-κB p65 expression was up-regulated in IR model, and NF-κB signaling pathway activation was associated with increased HMGB1. We further demonstrated that HMGB1 regulated the activation of NLRP3 and induced the processing of IL-1β in a NF-κB-dependent manner. However, caspase-8 was in the upstream of NF-κB, regulating its activation and subsequently the production of IL-1β. Overall, these results demonstrated that HMGB1 regulated the activation of NLRP3, subsequently the processing of IL-1β via NF-κB-pathway and HMGB1 also promoted the activation of caspase-8 which subsequently regulated the activation of NF-κB and then the processing of IL-1β.
In conclusion, our present study showed that HMGB1 release was increased in ischemic retinal tissue as early as 6 h after IR. Exogenous HMGB1 significantly promoted retinal IR injury. We also demonstrated that the novel caspase-8 inflammasome was activated after IR. HMGB1 led to the activation of canonical NLRP3 and non-canonical caspase-8 inflammasomes and the processing of IL-1β in retinal IR injury. In addition, HMGB1 regulated the NLRP3 activation and IL-1β maturation in NF-κB-dependent manner. HMGB1/capase-8 pathway promoted the activation of NF-κB, which subsequently induced the processing of IL-1β. Overall, HMGB1 plays a pivotal role in retinal IR injury through regulating the NLRP3 and caspase-8 inflammasomes activation and IL-1β maturation.
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made.
The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
YZ and WY designed the research. WC, HC, FL, and YZ performed the research; WC and HC analyzed the data. WC and HC wrote this paper. All authors read and approved the final manuscript.