The online version of this article (doi:10.1186/1477-7827-10-111) contains supplementary material, which is available to authorized users.
The authors have no competing interest to declare.
MI carried out the Immunohistochemical studies, and participated in the design and drafted the manuscript. YM carried out the production of the monoclonal antibodies and evaluated the result of immunohistochemical staining. KI participated in the design of the study, and conceived of the study, and participated in its design and consideration and drafted the manuscript. All authors have read and approved the final manuscript.
To investigate the role of prolyl hydroxylase (PH), a key enzyme of collagen synthesis, in human uterine leiomyoma, PH expression was determined in the normal uterine myometrium and the leiomyoma tissues during the menstrual cycle.
The tissues were obtained from 40 regularly cycling women (aged 29 to 53 yr) who were undergoing abdominal hysterectomy for symptomatic uterine leiomyoma. Immunohistochemistry for human PH with specific monoclonal antibody was used for analysis.
Immunohistochemical staining for PH revealed intense staining of leiomyoma cells in the uterine leiomyoma throughout the menstrual cycle, as compared with the adjacent normal myometrium. In the secretory phase, weak or no immunostaining for PH was detected in the normal myometrial tissues.
These results suggest that increased expression of PH might play an role in the physiology of uterine leiomyoma during the menstrual cycle.
Authors’ original file for figure 112958_2012_1052_MOESM1_ESM.tiff
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