Erschienen in:
01.04.2007
Hydrogen Peroxide in Exhaled Breath Condensate (EBC) vs Eosinophil Count in Induced Sputum (IS) in Parenchymal vs Airways Lung Diseases"
verfasst von:
Elizabeth Fireman, Moshe Shtark, Israel E. Priel, Robert Shiner, Ram Mor, Shmuel Kivity, Zvi Fireman
Erschienen in:
Inflammation
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Ausgabe 1-2/2007
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Abstract
We compared exhaled breath condensate (EBC) and induced sputum (IS) for assessing inflammation in pulmonary diseases in patients with obstructive lung disease (n = 20), persistent cough >6 months (n = 20), interstitial lung disease (n = 25) and controls (n = 10). EBC was collected by suspending a Teflon perfluoroalkoxy tube installed in an ice-filled container and connected to a polypropylene test tube. IS was recovered after 20’ inhalation of 3% saline with an ultrasonic nebulizer, and 300 cells were differentially counted in cytospin Giemsa-stained slides. H202 was measured by a method based on oxidation of phenolsulfonphthalein (phenol red) mediated by horseradish peroxidases and H202. Pulmonary function tests were performed by conventional methods. H202 levels in EBC and % eosinophils in IS were significantly different between groups. A positive and significant correlation was found between % eosinophils in IS and the levels of H202 in EBC for each group and for all patients combined.