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Erschienen in:
01.01.2008 | Original
Hyperglycemia and lipopolysaccharide decrease depression effect of interleukin 8 production by hypothermia: an experimental study with endothelial cells
Erschienen in: Intensive Care Medicine | Ausgabe 1/2008
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Objective
This study assessed whether hyperglycemia and lipopolysaccharide (LPS) decrease the depression effect of interleukin (IL) 8 production by hypothermia in endothelial cells.
Design and setting
Laboratory study in a university laboratory.
Subjects
Human umbilical vein endothelial cells (HUVECs).
Interventions
HUVECs were cultivated in various concentrations of glucose (5.5 or 16.5 mM = 100 or 300 mg/dl) with or without LPS stimulation for 5, 12, or 24 h at either 30° or 37 °C.
Results
After culturing, IL-8 mRNA expressions and IL-8 levels were measured. At 37 °C, hyperglycemia significantly increased basal IL-8 mRNA at 12 h and basal IL-8 at 24 h. At 37 °C hyperglycemia significantly increased LPS-stimulated IL-8 mRNA at 12 h and LPS-stimulated IL-8 at 12 and 24 h. At 30 °C basal IL-8mRNA, basal IL-8, and LPS-stimulated IL-8 were significantly decreased by hypothermia, but these hypothermic effects were not observed in LPS-stimulated IL-8 mRNA. Furthermore even at 30 °C hyperglycemia significantly increased LPS-stimulated IL-8 mRNA at all time points and LPS stimulated IL-8 at 24 h.
Conclusions
Hypothermia (30 °C) decreases the production of IL-8 in HUVECs but does not decrease the expression of IL-8 mRNA. When hypothermia is followed by hyperglycemia and LPS stimulation, such a combination may expose the patients to a high risk of secondary tissue damage during therapeutic hypothermia.