nach oben

Medical Oncology

Erschienen in:

01.02.2016 | Original Paper

Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT

verfasst von: Jianhong Zuo, Juan Wen, Mingsheng Lei, Meiling Wen, Sai Li, Xiu Lv, Zhaoyang Luo, Gebo Wen

Erschienen in: Medical Oncology | Ausgabe 2/2016

Einloggen, um Zugang zu erhalten

Abstract

The purpose of this study is to explore the role of hypoxia on the invasion and metastasis of laryngeal carcinoma. The invasion and migration ability of laryngeal cancer SCC10A cell was detected by transwell assay. Western blot was applied to analyze the expression of EMT-related proteins. Fifty-seven samples from postoperative patients with laryngeal cancer were collected to study. Immunohistochemistry was used to examine the expression of GLUT-1 and EMT-related proteins (Vim, E-cad, N-cad) in normal laryngeal squamous epithelial tissue, laryngeal cancer adjacent tissues and laryngeal squamous cell carcinoma tissues. Hypoxia promoted laryngeal cancer cell invasion and migration. Hypoxia also enhanced the expression of GLUT-1, vimentin and N-cad, which exist statistically significant correlation with the clinical staging and lymph node metastases (P < 0.05). The expression of GLUT-1 is positively correlated with Vim and N-cad expression in laryngeal squamous cell carcinoma tissues, but negatively correlated with E-cad expression. The patient survival rate with the positive expression of GLUT-1, Vim and N-cad becomes much shorter compared with those with negative expression of GLUT-1, Vim and N-cad (P < 0.05). Hypoxia promoted laryngeal cancer cell invasion and migration via EMT.

Xia C-X, Zhu Q, Zhao H-X, Yan F, Li S-L, Zhang S-M. Usefulness of ultrasonography in assessment of laryngeal carcinoma. Br J Radiol. 2013;86(1030):20130343.PubMedCentralCrossRefPubMed

Markou K, Christoforidou A, Karasmanis I, Tsiropoulos G, Triaridis S, Constantinidis I, Vital V, Nikolaou A. Laryngeal cancer: epidemiological data from Νorthern Greece and review of the literature. Hippokratia. 2013;17(4):313–8.PubMedCentralPubMed

Dan Yu, Jin C, Liu Y, Yang J, Zhao Y, Wang H, Zhao X, Cheng J, Liu X, Liu C. Clinical implications of cancer stem cell-like side population cells in human laryngeal cancer. Tumour Biol. 2013;34(6):3603–10.CrossRef

Harris AL. Hypoxia—a key regulatory factor in tumor growth. Nat Rev Cancer. 2002;2:38–47.CrossRefPubMed

Tsaiand JH, Yang J. Epithelial–mesenchymal plasticity in carcinoma metastasis. Genes Dev. 2013;27(20):2192–206.CrossRef

Mendichovszky I, Jackson A. Imaging hypoxia in gliomas. Br J Radiol. 2011;84(Spec Iss 2):S145–58.PubMedCentralCrossRefPubMed

Ponnusamy MP, Seshacharyulu P, Lakshmanan I, Vaz AP, Chugh S, Batra SK. Emerging role of mucins in epithelial to mesenchymal transition. Curr Cancer Drug Targets. 2013;13(9):945–56.PubMedCentralCrossRefPubMed

Steinestel K, Eder S, Schrader AJ, Steinestel J. Clinical significance of epithelial–mesenchymal transition. Clin Transl Med. 2014;3:17.PubMedCentralCrossRefPubMed

Hazan RB, Phillips GR, Qiao RF, Norton L, Aaronson SA. Exogenous expression of N-cadherin in breast cancer cells induces cell migration, invasion, and metastasis. J Cell Biol. 2000;148(4):779–90.PubMedCentralCrossRefPubMed

10.

Qian X, Anzovino A, Kim S, Suyama K, Yao J, Hulit J, Agiostratidou G, Chandiramani N, McDaid HM, Nagi C, Cohen HW, Phillips GR, Norton L, Hazan RB. N-cadherin/FGFR promotes metastasis through epithelial-to-mesenchymal transition and stem/progenitor cell-like properties. Oncogene. 2014;33(26):3411–21.PubMedCentralCrossRefPubMed

11.

Chung S, Yao J, Suyama K, Bajaj S, Qian X, Loudig OD, Eugenin EA, Phillips GR, Hazan RB. N-cadherin regulates mammary tumor cell migration through Akt3 suppression. Oncogene. 2013;32(4):422–30.PubMedCentralCrossRefPubMed

12.

Su Y, Li J, Witkiewicz AK, Brennan D, Neill T, Talarico J, Radice GL. N-cadherin haploinsufficiency increases survival in a mouse model of pancreatic cancer. Oncogene. 2012;31(41):4484–9.PubMedCentralCrossRefPubMed

13.

Kallergi G, Papadaki MA, Politaki E, Mavroudis D, Georgoulias V, Agelaki S. Epithelial to mesenchymal transition markers expressed in circulating tumour cells of early and metastatic breast cancer patients. Breast Cancer Res. 2011;13(3):R59.PubMedCentralCrossRefPubMed

14.

Ulirsch J, Fan C, Knafl G, Wu MJ, Coleman B, Perou CM, Swift-Scanlan T. Vimentin DNA methylation predicts survival in breast cancer. Breast Cancer Res Treat. 2013;. doi:10.1007/s10549-012-2353-5.PubMedCentralPubMed

15.

Wendt MK, Taylor MA, Schiemann BJ, Schiemann WP. Down-regulation of epithelial cadherin is required to initiate metastatic outgrowth of breast cancer. Mol Biol Cell. 2011;22(14):2423–35.PubMedCentralCrossRefPubMed

16.

Reinhold WC, Reimers MA, Lorenzi P, Ho J, Shankavaram UT, Ziegler MS, Bussey KJ, Nishizuka S, Ikediobi O, Pommier YG, Weinstein JN. Multifactorial regulation of E-cadherin expression: an integrative study. Mol Cancer Ther. 2010;9(1):1.PubMedCentralCrossRefPubMed

17.

Zuo J-h, Zhu W, Li Mao-Yu, Li X-H, Yi H, Zeng G-Q, Wan X-X, He Q-Y, Li J-H, Jia-Quan Q, Xiao Z-Q. Activation of EGFR promotes squamous carcinoma SCC10A cell migration and invasion via inducing EMT-like phenotype change and MMP-9-mediated degradation of E-cadherin. J Cell Biochem. 2011;112:2508–17.CrossRefPubMed

18.

Chou CC, Chuang HC, Salunke SB, Kulp SK, Chen CS. A novel HIF-1α-integrin-linked kinase regulatory loop that facilitates hypoxia-induced HIF-1α expression and epithelial–mesenchymal transition in cancer cells. Oncotarget. 2015;6(10):8271–85.PubMedCentralCrossRefPubMed

19.

Lu X, Kang Y. Hypoxia and hypoxia-inducible factors:master regulators of metastasis. Clin Cancer Res. 2010;16:5928–35.PubMedCentralCrossRefPubMed

20.

Majmundar AJ, Wong WJ, Simon MC. Hypoxia-inducible factors and the response to hypoxic stress. Mol Cell. 2010;40:294–309.PubMedCentralCrossRefPubMed

21.

Tsai YP, Wu KJ. Hypoxia-regulated target genes implicated in tumor metastasis. J Biomed Sci. 2012;19:102.PubMedCentralCrossRefPubMed

22.

Erler JT, Bennewith KL, Cox TR, Lang G, Bird D, Koong A, et al. Hypoxia-induced lysyl oxidase is a critical mediator of bone marrow cell recruitment to form the premetastatic niche. Cancer Cell. 2009;15:35–44.PubMedCentralCrossRefPubMed

23.

Huang X, Zuo J. Emerging roles of miR-210 and other non-coding RNAs in the hypoxic response. Acta Biochim Biophys Sin (Shanghai). 2014;46(3):220–32.CrossRef

24.

Starska K, Forma E, Jóźwiak P, Bryś M, Lewy-Trenda I, Brzezińska-Błaszczyk E, Krześlak A. Gene and protein expression of glucose transporter 1 and glucose transporter 3 in human laryngeal cancer-the relationship with regulatory hypoxia-inducible factor-1α expression, tumor invasiveness, and patient prognosis. Tumour Biol. 2015;36(4):2309–21.PubMedCentralCrossRefPubMed

25.

Cui Y, Nadiminty N, Liu C, Lou W, Schwartz CT, Gao AC. Upregulation of glucose metabolism by NF-κB2/p52 mediates enzalutamide resistance in castration-resistant prostate cancer cells. Endocr Relat Cancer. 2014;21:435–42.PubMedCentralCrossRefPubMed

26.

Rashmi R, DeSelm C, Helms C, Bowcock A, Rogers BE, Rader J, et al. AKT inhibitors promote cell death in cervical cancer through disruption of mTOR signaling and glucose uptake. PLoS ONE. 2014;9:e92948. doi:10.1371/journal.pone.0092948.PubMedCentralCrossRefPubMed

27.

Zhang C, Liu J, Liang Y, Wu R, Zhao Y, Hong X, et al. Tumour-associated mutant p53 drives the Warburg effect. Nat Commun. 2013;4:2935.PubMedCentralPubMed

28.

Chen XH, Bao YY, Zhou SH, Wang QY, Wei Y, Fan J. Glucose transporter-1 expression in CD133 + laryngeal carcinoma Hep-2 cells. Mol Med Rep. 2013;8:1695–700.PubMed

29.

Eckert AW, Lautner MH, Schütze A, Taubert H, Schubert J, Bilkenroth U. Coexpression of hypoxia-inducible factor-1α and glucose transporter-1 is associated with poor prognosis in oral squamous cell carcinoma patients. Histopathology. 2011;58:1136–47.CrossRefPubMed

30.

Zuo J, Ishikawa T, Boutros S, Xiao Z, Humtsoe JO, Kramer RH. Bcl-2 over-expression induces a partial epithelial to mesenchymal transition and promotes squamous carcinoma cell invasion and metastasis. Mol Cancer Res. 2010;8(2):170–82.CrossRefPubMed

31.

Zuo J, Wen M, Lei M, Peng X, Yang X, Liu Z. MiR-210 links hypoxia with cell proliferation regulation in human laryngocarcinoma cancer. J Cell Biochem. 2015;116(6):1039–49.CrossRefPubMed

32.

Yuan TZ, Zhang HH, Tang QF, Zhang Q, Li J, Liang Y, Huang LJ, Zheng RH, Deng J, Zhang XP. Prognostic value of kisspeptin expression in nasopharyngeal carcinoma. Laryngoscope. 2014;124(5):E167–74.CrossRefPubMed

33.

Li L, Wang J, Gao L, Gong L. Expression of paxillin in laryngeal squamous cell carcinoma and its prognostic value. Int J Clin Exp Pathol. 2015;8(8):9232–9.PubMedCentralPubMed

34.

Tu XP, Qiu QH, Chen LS, Luo XN, Lu ZM, Zhang SY, Chen SH. Preoperative neutrophil-to-lymphocyte ratio is an independent prognostic marker in patients with laryngeal squamous cell carcinoma. BMC Cancer. 2015;15(743):1–7.

35.

Xie J, Li D, Chen X, Wang F, Dong P. Expression and significance of hypoxia-inducible factor-1α and MDR1/P-glycoprotein in laryngeal carcinoma tissue and hypoxic Hep-2 cells. Oncol Lett. 2013;6(1):232–8.PubMedCentralPubMed

36.

Abdou AG, Eldien MM. Elsakka D.GLUT-1 expression in cutaneous basal and squamous cell carcinomas. Int J Surg Pathol. 2015;23(6):447–53.CrossRefPubMed

37.

Huang XQ, Chen X, Xie XX, Zhou Q, Li K, Li S, Shen LF, Su J. Co-expression of CD147 and GLUT-1 indicates radiation resistance and poor prognosis in cervical squamous cell carcinoma. Int J Clin Exp Pathol. 2014;7(4):1651–66.PubMedCentralPubMed

38.

Eckert AW, Lautner MH, Taubert H, Schubert J, Bilkenroth U. Expression of Glut-1 is a prognostic marker for oral squamous cell carcinoma patients. Oncol Rep. 2008;20:1381–5.PubMed

39.

Deron P, Vermeersch H, Mees G, Vangestel C, Pauwels P, Van de Wiele C. Expression and prognostic value of glucose transporters and hexokinases in tonsil and mobile tongue squamous cell carcinoma. Histol Histopathol. 2011;26:1165–72.PubMed

40.

Kondo Y, Yoshikawa K, Omura Y, Shinohara A, Kazaoka Y, Sano J, Mizuno Y, Yokoi T, Yamada S. Clinicopathological significance of carbonic anhydrase 9, glucose transporter-1, Ki-67 and p53 expression in oral squamous cell carcinoma. Oncol Rep. 2011;25:1227–33.PubMed

41.

Deron P, Vangestel C, Goethals I, De Potter A, Peeters M, Vermeersch H, Van de Wiele C. FDG uptake in primary squamous cell carcinoma of the head and neck. The relationship between overexpression of glucose transporters and hexokinases, tumour proliferation and apoptosis. Nuklearmedizin. 2011;50:15–21.CrossRefPubMed

42.

Zhang L, Huang G, Li X, Zhang Y, Jiang Y, Shen J, Liu J, Wang Q, Zhu J, Feng X, Dong J, Qian C. Hypoxia induces epithelial–mesenchymal transition via activation of SNAI1 by hypoxia-inducible factor-1α in hepatocellular carcinoma. BMC Cancer. 2013;13:108.PubMedCentralCrossRefPubMed

43.

Mayer A, Höckel M, Schlischewsky N, Schmidberger H, Horn LC, Vaupel P. Lacking hypoxia-mediated downregulation of E-cadherin in cancers of the uterine cervix. Br J Cancer. 2013;108(2):402–8.PubMedCentralCrossRefPubMed

44.

Huang X, Ding L, Bennewith KL, Tong RT, Welford SM, Ang KK, Story M, Le QT, Giaccia AJ. Hypoxia-inducible mir-210 regulates normoxic gene expression involved in tumor initiation. Mol Cell. 2009;35(6):856–67.PubMedCentralCrossRefPubMed

45.

Talbot LJ, Bhattacharya SD, Kuo PC. Epithelial-mesenchymal transition, the tumor microenvironment, and metastatic behavior of epithelial malignancies. Int J Biochem Mol Biol. 2012;3(2):117–36.PubMedCentralPubMed

46.

Huang SG, Zhang LL, Niu Q, Xiang GM, Liu LL, Jiang DN, Liu F, Li Y, Pu X. Hypoxia promotes epithelial–mesenchymal transition of hepatocellular carcinoma cells via inducing GLIPR-2 expression. PLoS ONE. 2013;8(10):e77497.PubMedCentralCrossRefPubMed

47.

Yang MH, Wu MZ, Chiou SH, Chen PM, Chang SY, Liu CJ, Teng SC, Wu KJ. Direct regulation of TWIST by HIF-1alpha promotes metastasis. Nat Cell Biol. 2008;10:295–305.CrossRefPubMed

48.

Choi JY, Jang YS, Min SY, Song JY. Overexpression of MMP-9 and HIF-1alpha in breast cancer cells under hypoxic conditions. J Breast Cancer. 2011;14:88–95.PubMedCentralCrossRefPubMed

49.

Kaidi A, Williams AC, Paraskeva C. Interaction between beta-catenin and HIF-1 promotes cellular adaptation to hypoxia. Nat Cell Biol. 2007;9:210–7.CrossRefPubMed

50.

Zhang W, Shi X, Peng Y, Wu M, Zhang P, Xie R, Wu Y, Yan Q, Liu S, Wang J. HIF-1α promotes epithelial–mesenchymal transition and metastasis through direct regulation of ZEB1 in colorectal cancer. PLoS ONE. 2015;10(6):e0129603.PubMedCentralCrossRefPubMed

Titel: Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT
verfasst von: Jianhong Zuo
Juan Wen
Mingsheng Lei
Meiling Wen
Sai Li
Xiu Lv
Zhaoyang Luo
Gebo Wen
Publikationsdatum: 01.02.2016
Verlag: Springer US
Erschienen in: Medical Oncology / Ausgabe 2/2016
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-015-0716-6

Neu im Fachgebiet Onkologie

16.04.2024 | Maligne primäre ZNS-Tumoren | Nachrichten

Springer Medizin

Hypoxia promotes the invasion and metastasis of laryngeal cancer cells via EMT

Abstract

Neu im Fachgebiet Onkologie

Manche Gestagene können das Risiko für Meningeome erhöhen

Einladung zum PSA-Screening senkt die Krebssterblichkeit

Chemotherapie mit Hindernissen

Das Ende der vollständigen axillären Lymphknotendissektion

Update Onkologie

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2016

Gene expression classifier for the diagnosis of indeterminate thyroid nodules: a meta-analysis

27-Hydroxycholesterol stimulates cell proliferation and resistance to docetaxel-induced apoptosis in prostate epithelial cells

Long noncoding RNAs: new insights into non-small cell lung cancer biology, diagnosis and therapy

Evodiamine induces apoptosis and enhances apoptotic effects of erlotinib in wild-type EGFR NSCLC cells via S6K1-mediated Mcl-1 inhibition

Tumor-associated macrophage or chemokine ligand CCL17 positively regulates the tumorigenesis of hepatocellular carcinoma

Gastrointestinal (GI) leiomyosarcoma (LMS) case series and review on diagnosis, management, and prognosis

Neu im Fachgebiet Onkologie

Manche Gestagene können das Risiko für Meningeome erhöhen

Einladung zum PSA-Screening senkt die Krebssterblichkeit

Chemotherapie mit Hindernissen

Das Ende der vollständigen axillären Lymphknotendissektion

Update Onkologie