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Osteoporosis International

Erschienen in:

01.01.2015 | Original Article

Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

verfasst von: Z.-B. Sun, J.-W. Wang, H. Xiao, Q.-S. Zhang, W.-S. Kan, F.-B. Mo, S. Hu, S.-N. Ye

Erschienen in: Osteoporosis International | Ausgabe 1/2015

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Abstract

Summary

In this study, we found out a previously undefined function of icariin which restored the dynamic balance between osteogenic and adipogenic differentiation of mesenchymal stem cells (MSCs) in patients with osteonecrosis of femoral head (ONFH) via ABCB1-promoter demethylation. These findings provided important information regarding potential implication of icariin targeting epigenetic changes for the treatment of steroid -associated ONFH.

Introduction

Here, we investigated whether icariin can also exert a beneficial role in the reactivation of MSCs in the patients with steroid-associated ONFH via ABCB1-promoter demethylation.

Methods

Bone marrow was collected from the proximal femur in patients with steroid-associated ONFH (n = 20) and patients with new femoral neck fractures (n = 22), and then MSCs were isolated. We investigated cell viability, intracellular reactive oxygen species (ROS) level, mitochondrial membrane potential (MMP), P-glycoprotein (P-gp) activity, the transcript levels of ABCB1 and oxidative stress-related genes, methylation extent at CpG islands of ABCB1 promoter, and osteogenic and adipogenic differentiation ability of MSCs from the femoral neck fractures group and from the steroid-associated ONFH group treated with or without icariin.

Results

We observed that MSCs from the steroid-associated ONFH group showed reduced proliferation ability, elevated ROS level, depressed MMP, weakened osteogenesis, and enhanced adipogenesis while low P-gp activity, transcription level of ABCB1, and oxidative stress-related genes as well as aberrant CpG islands hypermethylation of ABCB1 were also noted in steroid-associated ONFH group. Treatment with icariin obviously induced de novo P-gp expression, decreased oxidative stress, and promoted osteogenesis.

Conclusion

Icariin may be a potential drug targeting epigenetic changes for the treatment of steroid-associated ONFH.

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Titel: Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study
verfasst von: Z.-B. Sun
J.-W. Wang
H. Xiao
Q.-S. Zhang
W.-S. Kan
F.-B. Mo
S. Hu
S.-N. Ye
Publikationsdatum: 01.01.2015
Verlag: Springer London
Erschienen in: Osteoporosis International / Ausgabe 1/2015
Print ISSN: 0937-941X
Elektronische ISSN: 1433-2965
DOI: https://doi.org/10.1007/s00198-014-2809-z

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Icariin may benefit the mesenchymal stem cells of patients with steroid-associated osteonecrosis by ABCB1-promoter demethylation: a preliminary study

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Summary

Introduction

Methods

Results

Conclusion

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