Background
Methods
Collection and inclusion criteria of studies
Microarray data
Data processing
Functional and pathway enrichment analysis
PPI network construction and analysis of modules
Prediction of miRNA targets
Results
Identification of DEGs
Functional and pathway enrichment analyses
Category | Term | Count | % |
P Value |
---|---|---|---|---|
Up-regulated | ||||
GOTERM_BP_FAT | GO:0007059 ~ chromosome segregation | 9 | 17.6 | 2.66E-06 |
GOTERM_BP_FAT | GO:0098813 ~ nuclear chromosome segregation | 8 | 15.7 | 1.06E-05 |
GOTERM_BP_FAT | GO:0000819 ~ sister chromatid segregation | 7 | 13.7 | 2.91E-05 |
GOTERM_BP_FAT | GO:0051301 ~ cell division | 9 | 17.6 | 1.31E-04 |
GOTERM_BP_FAT | GO:0007067 ~ mitotic nuclear division | 8 | 15.7 | 1.44E-04 |
GOTERM_CC_FAT | GO:0000793 ~ condensed chromosome | 5 | 9.8 | 2.14E-03 |
GOTERM_CC_FAT | GO:0005829 ~ cytosol | 18 | 35.3 | 3.98E-03 |
GOTERM_CC_FAT | GO:0005578 ~ proteinaceous extracellular matrix | 5 | 9.8 | 1.51E-02 |
GOTERM_CC_FAT | GO:0098651 ~ basement membrane collagen trimer | 2 | 3.9 | 2.08E-02 |
GOTERM_CC_FAT | GO:0005694 ~ chromosome | 7 | 13.7 | 3.38E-02 |
GOTERM_MF_FAT | GO:0042826 ~ histone deacetylase binding | 3 | 5.9 | 3.03E-02 |
KEGG_PATHWAY | hsa04512:ECM-receptor interaction | 3 | 5.9 | 1.68E-02 |
Down-regulated | ||||
GOTERM_BP_FAT | GO:0006956 ~ complement activation | 6 | 10.0 | 1.83E-05 |
GOTERM_BP_FAT | GO:0072376 ~ protein activation cascade | 6 | 10.0 | 6.43E-05 |
GOTERM_BP_FAT | GO:0019752 ~ carboxylic acid metabolic process | 12 | 20.0 | 8.21E-05 |
GOTERM_BP_FAT | GO:0043436 ~ oxoacid metabolic process | 12 | 20.0 | 8.66E-05 |
GOTERM_BP_FAT | GO:0006955 ~ immune response | 16 | 26.7 | 1.23E-04 |
GOTERM_CC_FAT | GO:0005581 ~ collagen trimer | 4 | 6.7 | 7.15E-03 |
GOTERM_CC_FAT | GO:0072562 ~ blood microparticle | 4 | 6.7 | 1.91E-02 |
GOTERM_CC_FAT | GO:0005579 ~ membrane attack complex | 2 | 3.3 | 2.67E-02 |
GOTERM_CC_FAT | GO:0031988 ~ membrane-bounded vesicle | 21 | 35.0 | 4.60E-02 |
GOTERM_CC_FAT | GO:0044421 ~ extracellular region part | 22 | 36.7 | 5.00E-02 |
GOTERM_MF_FAT | GO:0005496 ~ steroid binding | 4 | 6.7 | 3.47E-03 |
GOTERM_MF_FAT | GO:0070330 ~ aromatase activity | 3 | 5.0 | 3.57E-03 |
GOTERM_MF_FAT | GO:0016712 ~ oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen | 3 | 5.0 | 4.11E-03 |
GOTERM_MF_FAT | GO:0004497 ~ monooxygenase activity | 4 | 6.7 | 4.77E-03 |
GOTERM_MF_FAT | GO:0004252 ~ serine-type endopeptidase activity | 5 | 8.3 | 7.38E-03 |
KEGG_PATHWAY | hsa05204:Chemical carcinogenesis | 6 | 10.0 | 4.53E-05 |
KEGG_PATHWAY | hsa00140:Steroid hormone biosynthesis | 4 | 6.7 | 3.04E-03 |
KEGG_PATHWAY | hsa00830:Retinol metabolism | 4 | 6.7 | 4.19E-03 |
KEGG_PATHWAY | hsa00982:Drug metabolism - cytochrome P450 | 4 | 6.7 | 4.76E-03 |
PPI network construction and analysis of modules
Gene symbol | Node degree | Related DEMs |
---|---|---|
TOP2A | 30 | none |
FOS | 27 | hsa-miR-221 |
TK1 | 24 | none |
CDC20 | 23 | none |
ESR1 | 21 | hsa-miR-148b, hsa-miR-221, hsa-miR-18a, hsa-miR-181b, hsa-miR-19a |
CCNB2 | 18 | none |
CXCL12 | 17 | hsa-miR-221 |
FOXO1 | 16 | hsa-miR-135b, hsa-miR-324-3p, hsa-miR-369-3p |
HMMR | 15 | none |
VWF | 15 | none |
ACSM3 | 14 | none |
COL4A1 | 14 | none |
ZIC2 | 14 | none |
RFC4 | 13 | none |
TXNRD1 | 13 | none |
GNAO1 | 12 | hsa-miR-193a |
CYP3A4 | 10 | none |
RAP2A | 10 | none |
Category | Term | Count | % |
P Value |
---|---|---|---|---|
GOTERM_BP_FAT | GO:0000302 ~ response to reactive oxygen species | 4 | 26.7 | 8.25E-04 |
GOTERM_BP_FAT | GO:1901700 ~ response to oxygen-containing compound | 7 | 46.7 | 1.06E-03 |
GOTERM_BP_FAT | GO:0006979 ~ response to oxidative stress | 4 | 26.7 | 4.59E-03 |
GOTERM_BP_FAT | GO:0048545 ~ response to steroid hormone | 4 | 26.7 | 4.78E-03 |
GOTERM_BP_FAT | GO:1903047 ~ mitotic cell cycle process | 5 | 33.3 | 5.21E-03 |
GOTERM_CC_FAT | GO:0005654 ~ nucleoplasm | 9 | 60.0 | 1.54E-03 |
GOTERM_CC_FAT | GO:0005829 ~ cytosol | 8 | 53.3 | 1.94E-02 |
GOTERM_CC_FAT | GO:0005694 ~ chromosome | 4 | 26.7 | 4.75E-02 |
GOTERM_MF_FAT | GO:0044877 ~ macromolecular complex binding | 9 | 60.0 | 4.65E-06 |
GOTERM_MF_FAT | GO:0003682 ~ chromatin binding | 6 | 40.0 | 5.72E-05 |
GOTERM_MF_FAT | GO:0019899 ~ enzyme binding | 6 | 40.0 | 1.67E-02 |
GOTERM_MF_FAT | GO:0000982 ~ transcription factor activity, RNA polymer | 3 | 20.0 | 3.72E-02 |
GOTERM_MF_FAT | GO:0097367 ~ carbohydrate derivative binding | 6 | 40.0 | 4.13E-02 |
KEGG_PATHWAY | hsa04512:ECM-receptor interaction | 3 | 20.0 | 9.53E-03 |
miRNA-DEG pairs
miRNA | Adj. P
| logFC | Target genes |
---|---|---|---|
hsa-mir-106b | 1.94E-41 | 1.49 | ANKRD29, IL1RAP, ADARB1, ARID4B, EPHA4, FBXL5, PDCD1LG2, PKD2, PTPN4, SLC40A1 |
hsa-mir-148b | 9.59E-22 | 1.37 | ESR1, ABCB7, B4GALT6, CDK19, LDLR, MXD1, NPTN, RPS6KA5, SOS2, SZRD1 |
hsa-mir-151 | 2.61E-21 | 1.14 | AGO2, ZFAND5, CLK1, AQP4, CASD1, RPS6KA5, FAM104A, NAA15, YTHDF3, HIF1A |
hsa-mir-221 | 7.37E-21 | 1.66 | ESR1, FOS, CXCL12, CDKN1B, GABRA1, PANK3, TCF12, HECTD2, RFX7, TMCC1 |
hsa-mir-18a | 2.16E-20 | 2.48 | ESR1, NEDD9, BBX, INADL, MAP7D1, PHF19, RORA, ZBTB47, CDK19, DICER1 |
hsa-mir-200b | 4.50E-17 | −3.15 | MAGEC2, ESM1, TCEB1, TRIM33, LHFP, PTPN21, ARHGAP6, VASH2, HIPK3, NR5A2 |
hsa-mir-224 | 4.85E-15 | −71.38 | NRCAM, CPNE8, ZNF207, ACSL4, RNF144B, SH3KBP1, RNF38, SLC4A4, GPR158, GGNBP2 |
hsa-mir-200a | 5.03E-14 | −2.29 | COL15A1, MYBL1, ZEB2, ATP8A1, DCP2, TMEM170B, ZBTB34, DUSP3, TRHDE, RSAD2 |
hsa-mir-182 | 1.41E-06 | −2.69 | NRCAM, ABHD13, MFAP3, PCNX, NADK2, CAMSAP2, SLC39A9, NCALD, ANK3, HOXA9 |
hsa-mir-142-5p | 6.59E-06 | −1.49 | ANKRD29, IGF2BP3, IGSF3, ZFP36, ZFPM2, BAI3, AFF4, DIAPH2, AHR, ARID4B |