Background
Methods
Patients enrolled in the study
Comprehensive analysis of miRNA expression and signature identification
RNA extraction and qPCR analysis
Statistical analyses
Results
Identification of miRNA signature for NAC response of ESCC using a microarray-based miRNA expression profiling
qPCR verification with serum specimens taken from ESCC patients treated with NAC
Characteristics | Value | |
---|---|---|
Response to preoperative chemotherapy | 0, 1/2, 3 | 69/15 |
Age | Years | 65 (30–77) |
Sex | F/M | 14/70 |
cT | 0/1/2/3/4 | 0/11/12/59/2 |
cN | 0/1/2/3 | 10/47/23/4 |
cM | 0/1 | 79/5 |
cStage | 0/1/2/3/4 | 0/3/22/54/5 |
pT | 0/1/2/3/4 | 4/23/19/29/9 |
pN | 0/1/2/3 | 29/28/16/11 |
pM | 0/1 | 79/5 |
pStage | 0/1/2/3/4 | 2/17/27/33/5 |
Differentiationa | Poor/moderate/well | 10/59/8 |
Lymphatic invasion | 0/1 | 31/53 |
Venous invasion | 0/1 | 59/25 |
Pretreatment serum CEAb | ng/ml | 2.53 (1–6) |
Pretreatment serum SCC antigenc | ng/ml | 1.46 (0.5–6.7) |
Variables | Response |
P
| |
---|---|---|---|
Grade 0, 1 | Grade 2, 3 | ||
Sex | |||
Female | 12 | 2 | 1.00 |
Male | 57 | 13 | |
Age | |||
Years | 65 (41–77) | 66 (30–76) | 0.56 |
cT | |||
1 | 8 | 3 | 0.78 |
2 | 10 | 2 | |
3 | 49 | 10 | |
4 | 2 | 0 | |
cN | |||
0 | 8 | 2 | 0.80 |
1 | 38 | 9 | |
2 | 20 | 3 | |
3 | 3 | 1 | |
cStage | |||
1 | 2 | 1 | 0.75 |
2 | 18 | 4 | |
3 | 45 | 9 | |
4 | 4 | 1 | |
pT | |||
0 | 0 | 4 | > 0.0001 |
1 | 17 | 6 | |
2 | 15 | 4 | |
3 | 29 | 0 | |
4 | 8 | 1 | |
pN | |||
0 | 24 | 5 | 0.90 |
1 | 22 | 6 | |
2 | 13 | 3 | |
3 | 10 | 1 | |
pStage | |||
0 | 0 | 2 | 0.07 |
1 | 14 | 3 | |
2 | 21 | 6 | |
3 | 29 | 4 | |
4 | 5 | 0 | |
Differentiation | |||
Well or moderate | 56 | 11 | 0.340 |
Poor | 10 | 0 | |
Lymphatic invasion | |||
< 2 | 23 | 8 | 0.250 |
≥ 2 | 46 | 7 | |
Venous invasion | |||
< 20 | 50 | 9 | 0.36 |
≥ 20 | 19 | 6 | |
Pretreatment CEA | |||
ng/ml | 2.6 (1.0–6.0) | 1.9 (0.4–4.3) | 0.060 |
Pretreatment SCC | |||
ng/ml | 1.1 (0.5–6.4) | 1.4 (0.6–6.7) | 0.350 |
Prognostic value of the selected miRNAs’ expression
Evaluation of selected miRNAs and combined models as biomarkers for NAC response
Variables | OR | 95% CI Low | 95% CI High |
P
|
---|---|---|---|---|
Age (years) | ||||
≥ 65 vs < 65 | 0.55 | 0.17 | 1.8 | 0.31 |
Sex | ||||
Male vs female | 0.73 | 0.15 | 3.7 | 0.70 |
cT | ||||
3, 4 vs 0, 1, 2 | 1.42 | 0.43 | 4.7 | 0.57 |
cN | ||||
Positive vs negative | 1.17 | 0.22 | 6.2 | 0.85 |
Serum SCC antigen (ng/ml) | ||||
≥ 1.5 vs < 1.5 | 0.39 | 0.13 | 1.2 | 0.11 |
miR-23a-5p | ||||
High vs low | 2.80 | 0.90 | 8.8 | 0.08 |
miR-193b-5p | ||||
High vs low | 7.47 | 0.93 | 60.2 | 0.06 |
miR-873-3p | ||||
High vs low | 3.11 | 0.96 | 10.1 | 0.06 |