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19.10.2018 | Original Article

Identification of genes associated with survival of breast cancer patients

Zeitschrift:
Breast Cancer
Autoren:
Min Liu, Siying Zhou, Jinyan Wang, Qian Zhang, Sujin Yang, Jifeng Feng, Bin Xu, Shanliang Zhong
Wichtige Hinweise

Electronic supplementary material

The online version of this article (https://​doi.​org/​10.​1007/​s12282-018-0926-9) contains supplementary material, which is available to authorized users.
Min Liu and Siying Zhou are equal contributors.

Abstract

Background

We aimed to investigate the potential of microRNA expression profiles to predict survival in breast cancer.

Methods

MicroRNA and mRNA expression data of breast cancer were downloaded from The Cancer Genome Atlas. LASSO regression was used to identify microRNAs signature predicting survival of breast cancer patients. Transfection experiment was conducted to explore the influence of microRNAs on their potential targets.

Results

We identified 56 differentially expressed microRNAs in breast cancer tissues compared to adjacent normal tissues. 10 microRNAs with non-zero coefficient were selected from the 56 microRNAs using LASSO Cox regression. After predicting the targets for the 10 microRNAs, we further obtained 155 targets that were associated with overall survival of breast cancer patients. Spearman’s correlation analysis found that the expression of SCUBE2, SCRN3, YTHDF3, ITFG1, ITPRIPL2, and JAK1 was an inversely correlated with their microRNAs. Transfection experiment showed that YTHDF3 was down-regulated in cells transfected with miR-106b-5p mimics compared with those transfected with negative control of mimics (fold change 4.21; P < 0.01).

Conclusions

In conclusion, we identified a 10-miRNA signature associated with prognosis of breast cancer patients. The expression of YTHDF3 was down-regulated by miR-106b-5p.

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Zusatzmaterial
Figure S1 Overall survival analysis of breast cancer patients with high-expression genes and low-expression genes. The number following “=” is the median survival time for each group (509 patients for each group) (TIF 561 KB)
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Figure S2 MiR-9-5p and miR-106b-5p binding sites in the six candidate target genes (TIF 323 KB)
12282_2018_926_MOESM2_ESM.tif
Supplementary material 3 (DOCX 14 KB)
12282_2018_926_MOESM3_ESM.docx
Supplementary material 4 (XLSX 14 KB)
12282_2018_926_MOESM4_ESM.xlsx
Supplementary material 5 (XLSX 44 KB)
12282_2018_926_MOESM5_ESM.xlsx
Supplementary material 6 (XLSX 22 KB)
12282_2018_926_MOESM6_ESM.xlsx
Literatur
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