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Medical Oncology

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01.06.2010 | Original Paper

Identification of human gastric carcinoma biomarkers by differential protein expression analysis using ¹⁸O labeling and NanoLC-MS/MS coupled with laser capture microdissection

verfasst von: Zhang ZhiQiang, Li MaoYu, Zhang GuiYing, Pen Fang, Yao HuiXing, Xiao ZhiQiang, Chen ZhuChu

Erschienen in: Medical Oncology | Ausgabe 2/2010

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Abstract

Early detection and rational therapy for gastric cancer are crucial. In this study we undertook comparative proteomics for identification of gastric carcinoma biomarkers using pooled laser capture microdissected GA cells and matched nonmalignant gastric mucosa epithelial cells. The method involved separation of total proteins by 1D SDS-PAGE, trypsin digestion, and postdigest ¹⁸O/¹⁶O labeling followed by nano-HPLC-MS/MS for peptide identification and relative quantification. A total of 78 differentially expressed proteins were identified, among these proteins, 42 proteins were up-regulated in GA and 36 proteins were down-regulated. Some differentially expressed proteins were further validated by western blot analysis.

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Titel: Identification of human gastric carcinoma biomarkers by differential protein expression analysis using 18O labeling and NanoLC-MS/MS coupled with laser capture microdissection
verfasst von: Zhang ZhiQiang
Li MaoYu
Zhang GuiYing
Pen Fang
Yao HuiXing
Xiao ZhiQiang
Chen ZhuChu
Publikationsdatum: 01.06.2010
Verlag: Springer US
Erschienen in: Medical Oncology / Ausgabe 2/2010
Print ISSN: 1357-0560
Elektronische ISSN: 1559-131X
DOI: https://doi.org/10.1007/s12032-009-9208-x

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Identification of human gastric carcinoma biomarkers by differential protein expression analysis using ¹⁸O labeling and NanoLC-MS/MS coupled with laser capture microdissection

Abstract

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ICI-Therapie in der Schwangerschaft wird gut toleriert

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

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