Background
Methods
Construction of the network
Degree distribution
Identification of hubs
Identification of date and party hubs
Analysis of functional similarity
Calculation of cumulative centrality score
Construction of local sub graph
Calculation of global and local network perturbation score
Correlation of different scores
Stage Specific interactions
Results and discussion
Construction and validation of the PPI network
Network parameters
|
MN
|
MRN
|
EN
|
ERN
|
---|---|---|---|---|
No of nodes
| 1607 | 1607 | 1505 | 1505 |
No of edges
| 4750 | 4750 | 4085 | 4085 |
Average degree
| 5.9 | 5.2 | 5.34 | 5.1 |
Average shortest path
| 4.39 | 4.5 | 4.14 | 4.5 |
No of hubs
| 177 | 612 | 126 | 526 |
Degree threshold for defining hub
| 15 | 11 | 15 | 9 |
Average clustering coefficient
| 0.12 | 0.001 | 0.07 | 0.006 |
Max degree
| 77 | 18 | 61 | 16 |
Diameter
| 12 | 9 | 14 | 9 |
Identification and classification of hubs
Identification of central proteins
Identification of GNPP and LNPP
Identification of important interacting proteins (IIPs)
Stage specific networks
Name of stage
|
For MN
|
For MN-14
| ||||
---|---|---|---|---|---|---|
(Node:1605, Interaction:4750)
|
(Node:303, Interaction:523)
| |||||
Number of stage specific interaction
|
Number of stage specific node
|
Number of unique interaction
|
Number of stage specific interaction
|
Number of stage specific nodes
|
Number of unique interaction
| |
Sporozoite
| 1458 | 617 | 9 | 248 | 140 | 2 |
Merozoite
| 1155 | 438 | 1 | 190 | 100 | 0 |
Trophozoite
| 3074 | 1126 | 66 | 378 | 218 | 1 |
Ring stage
| 2638 | 909 | 35 | 364 | 204 | 2 |
Schizont
| 3079 | 1132 | 99 | 388 | 225 | 4 |
Gametocyte
| 2635 | 1062 | 105 | 334 | 201 | 7 |
Host interacting proteins
Number of interactors in Human
|
Number of interaction between Human and
Plasmodium
proteins
|
Number of interactor in
Plasmodium
(MN)
|
Number of intra-pathogen interaction
| |
---|---|---|---|---|
Plasmodium
proteins having host partners152
| 257 | 367 | 515 | 996 |
IIP-271 having interacting host partner 35
| 77 | 103 | 351 | 541 |