Background
Methods
Reagents, cell lines, tissue culture and quantitative reverse transcription-PCR
Mouse xenograft studies
Nuclear and chromatin extraction and western blots
Patient samples
Immunofluorescence (IF) and Immunohistochemistry (IHC)
Generation of the H2B-GFP tagged line and label retention assay
Results
Bortezomib-surviving MM cells display a CDK6LOW/p21HIGH quiescent profile
A Low-dose 5-aza-cytidine treatment potentiates Bz-induced cell death and deep quiescence
Upregulation of GRP78 in Bz-surviving MM cells is associated with disease progression in patients and therapy-mediated cell death
MM code | Age at diagnosis | Ouant Immunoglobulins and Serum immunofixation at collection | Albumin | LDH | Prior Bortezomib? | Myeloma status at time of Collection |
---|---|---|---|---|---|---|
MM16 | 64 | lgG 2371, monoclonal protein in gamma region | 4.3 | 201 | no | Stable disease |
MM17 | 59 | IgG 282, IgA 5083, IgM 16, Two IgA lambda monoclonal band seen, representing 85 % | 3.7 | 106 | yes | Stable disease |
MM18 | 68 | IgG 2333, IgA 12, 1gM 20, monoclonal protein seen in gamma region | 3.3 | 175 | yes | Progression of Disease (increase in M-spike) |
MM19 | 46 | IgG 4656, IgA 27, 1 gM 53, monoclonal protein in gamma region | 3.8 | 142 | no | Minimal response/stable disease |
MM20 | unav | IgG 4099, monoclonal band in gamma region | 3.7 | 121 | unav | Stable Disease |
MM21 | 56 | IgG 314, 1 gM <5, IgA <5, faint free kappa band | 4.7 | 143 | yes | Progression of Disease |
MM22 | 52 | IgG 453, 1 gM 7, IgA 132, faint IgG lambda band | 2.7 | 170 | yes | partial response/stable disease |
MM23 | 70 | lgG 840, IgA 9, 1 gM 9, lgG kappa monoclonal spike seen, representing all of monoclonal protein | 4.5 | 564 | yes | Remission (very good partial response) |
MM24 | 57 | lgG 255, 1 gM 12, IgA 19, faint free Lambda band seen | 3.1 | 154 | yes | Progression of Disease |
MM25 | 39 | lgG 188, IgA 10, 1gM 12, Free monoclonal lambda light chain, normal Igs greatly diminished | 4.7 | 601 | yes | Progression of Disease |
MM26 | 66 | lgG 6589, Iga 8, 1 gM <5, lgG kappa monoclonal representing all of total | 3.9 | 166 | yes | Progression of Disease |
MM27 | 54 | lgG 5543, IgA 11, 1 gM 19, lgG kappa monoclonal protein | 4.1 | 210 | yes | Progression of Disease |