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Clinical Oral Investigations

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06.05.2020 | Original Article

Identification of microorganisms in irreversible pulpitis and primary endodontic infections with respect to clinical and radiographic findings

verfasst von: Nazanin Zargar, Hengameh Ashraf, S. M. Amin Marashi, Mohammad Sabeti, Alireza Aziz

Erschienen in: Clinical Oral Investigations | Ausgabe 6/2020

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Abstract

Objectives

The aim of this study was to evaluate the composition of microbiota of irreversible pulpitis and primary endodontic infections with respect to clinical and radiographic findings by performing cultures and 16s rDNA sequencing in Iranian patients.

Material and methods

In this prospective cross-sectional study, samples were collected from 41 root canals for 4 main groups of patients. Bacterial identification was performed by the polymerase chain reaction (PCR) and 16s rDNA sequencing of aerobic and anaerobic cultivable colonies taken from patients’ culture plates. Additionally, the presence of 13 bacterial species and 3 nonbacterial species was also explored using PCR and species-specific primers.

Results

Sixteen microbial species, 1 fungus (Candida albicans), and 1 virus (Herpes simplex virus) were discovered and isolated. Species with the highest prevalence were Dialister invisus (68.3%), Porphyromonas gingivalis (58.8%), Streptococcus salivarius (58.5%), and Treponema denticola (56.1%). Lysinibacillus fusiformis (19.1%) was detected in the root canals for the first time. Candida albicans was seen in 11 cases (26.8%). Herpes simplex virus (HSV) was seen in 4 patients (9.8%).

Conclusions

Our results suggest that Gram-negative anaerobic oral bacteria are the majority of the microbes in primary endodontic infections. Various combinations of bacterial species were related to different clinical and radiographic conditions. Lysinibacillus fusiformis was detected for the first time in primary endodontic infections.

Clinical relevance

The results of this investigation might help clinicians choose to identify suspected endodontic pathogens in the etiology of each form of pulpal and periradicular diseases to determine the best therapeutic measures.

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Titel: Identification of microorganisms in irreversible pulpitis and primary endodontic infections with respect to clinical and radiographic findings
verfasst von: Nazanin Zargar
Hengameh Ashraf
S. M. Amin Marashi
Mohammad Sabeti
Alireza Aziz
Publikationsdatum: 06.05.2020
Verlag: Springer Berlin Heidelberg
Erschienen in: Clinical Oral Investigations / Ausgabe 6/2020
Print ISSN: 1432-6981
Elektronische ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-019-03075-9

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Identification of microorganisms in irreversible pulpitis and primary endodontic infections with respect to clinical and radiographic findings

Abstract

Objectives

Material and methods

Results

Conclusions

Clinical relevance

Neu im Fachgebiet Zahnmedizin

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Springer Medizin

Abstract

Objectives

Material and methods

Results

Conclusions

Clinical relevance

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