Skip to main content
main-content

01.11.2010 | Original Article | Ausgabe 9/2010

Annals of Nuclear Medicine 9/2010

Imaging of a rat osteoarthritis model using 18F-fluoride positron emission tomography

Zeitschrift:
Annals of Nuclear Medicine > Ausgabe 9/2010
Autoren:
Yusuke Umemoto, Takushi Oka, Tomio Inoue, Tomoyuki Saito

Abstract

Objective

Currently, conventional radiography is the standard method for the diagnosis and evaluation of the severity of osteoarthritis (OA), but it takes a couple of years to detect cartilage loss. Magnetic resonance imaging can delineate articular cartilage and accurately assess cartilage volume and thickness, but its reliability for very early diagnosis of OA is still controversial. The purpose of this study was to confirm the potential of 18F-fluoride PET for the early diagnosis of OA by using a surgically induced rat OA model.

Methods

Seventeen 16-week-old male Sprague-Dawley rats underwent anterior cruciate ligament transection (ACLT) in the right knee to induce OA. The left knee underwent sham operation. At 2, 4, and 8 weeks after operation, the rats were injected with 2.5 MBq/kg of 18F-fluoride, and 30 min after injection, each rat was killed and the bilateral knees were resected. The femur and tibia were cut horizontally, approximately 2 mm from the joint surface excluding the growth plate, and were cut into the medial and lateral condyles. The patella was also resected and blood samples were collected. The radioactivity of each sample was measured by gamma counting. Assays for serum cartilage oligomeric matrix protein and serum C-telopeptide of type II collagen were performed. Histopathological grading was performed according to a modified Mankin’s scoring system. Two rats underwent PET scans at 2, 4, and 8 weeks after operation. The rats were injected with 30 MBq of 18F-fluoride, and 30 min after injection, bilateral knee images with a 30-min acquisition time were obtained with an animal PET system.

Results

The uptake of 18F-fluoride was significantly higher in ACLT knees than sham-operated knees in the medial femur and medial tibia at 2 weeks after operation. At 4 weeks after operation, the medial femur, medial tibia, and lateral tibia of OA knees showed significantly higher uptake of 18F-fluoride compared with sham-operated knees. At 8 weeks, all sections showed significant differences. The uptake of 18F-fluoride significantly increased as time elapsed in all sections. Uptake showed a significant correlation with histological scores.

Conclusion

Our results suggest that 18F-fluoride is potentially useful for the early detection of osteoarthritic changes.

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

★ PREMIUM-INHALT
e.Med Interdisziplinär

Mit e.Med Interdisziplinär erhalten Sie Zugang zu allen CME-Fortbildungen und Fachzeitschriften auf SpringerMedizin.de. Zusätzlich können Sie eine Zeitschrift Ihrer Wahl in gedruckter Form beziehen – ohne Aufpreis.

Weitere Produktempfehlungen anzeigen
Literatur
Über diesen Artikel

Weitere Artikel der Ausgabe 9/2010

Annals of Nuclear Medicine 9/2010 Zur Ausgabe
  1. Das kostenlose Testabonnement läuft nach 14 Tagen automatisch und formlos aus. Dieses Abonnement kann nur einmal getestet werden.

  2. Das kostenlose Testabonnement läuft nach 14 Tagen automatisch und formlos aus. Dieses Abonnement kann nur einmal getestet werden.