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European Journal of Nuclear Medicine and Molecular Imaging

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28.05.2022 | Original Article

Imaging the fetal nonhuman primate brain with SV2A positron emission tomography (PET)

verfasst von: Samantha Rossano, Takuya Toyonaga, Eric Berg, Isabella Lorence, Krista Fowles, Nabeel Nabulsi, Jim Ropchan, Songye Li, Yunpeng Ye, Zachary Felchner, David Kukis, Yiyun Huang, Helene Benveniste, Alice F. Tarantal, Stephanie Groman, Richard E. Carson

Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging | Ausgabe 11/2022

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Abstract

Purpose

Exploring synaptic density changes during brain growth is crucial to understanding brain development. Previous studies in nonhuman primates report a rapid increase in synapse number between the late gestational period and the early neonatal period, such that synaptic density approaches adult levels by birth. Prenatal synaptic development may have an enduring impact on postnatal brain development, but precisely how synaptic density changes in utero are unknown because current methods to quantify synaptic density are invasive and require post-mortem brain tissue.

Methods

We used synaptic vesicle glycoprotein 2A (SV2A) positron emission tomography (PET) radioligands [¹¹C]UCB-J and [¹⁸F]Syn-VesT-1 to conduct the first assessment of synaptic density in the developing fetal brain in gravid rhesus monkeys. Eight pregnant monkeys were scanned twice during the third trimester at two imaging sites. Fetal post-mortem samples were collected near term in a subset of subjects to quantify SV2A density by Western blot.

Results

Image-derived fetal brain SV2A measures increased during the third trimester. SV2A concentrations were greater in subcortical regions than in cortical regions at both gestational ages. Near term, SV2A density was higher in primary motor and visual areas than respective associative regions. Post-mortem quantification of SV2A density was significantly correlated with regional SV2A PET measures.

Conclusion

While further study is needed to determine the exact relationship of SV2A and synaptic density, the imaging paradigm developed in the current study allows for the effective in vivo study of SV2A development in the fetal brain.

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Titel: Imaging the fetal nonhuman primate brain with SV2A positron emission tomography (PET)
verfasst von: Samantha Rossano
Takuya Toyonaga
Eric Berg
Isabella Lorence
Krista Fowles
Nabeel Nabulsi
Jim Ropchan
Songye Li
Yunpeng Ye
Zachary Felchner
David Kukis
Yiyun Huang
Helene Benveniste
Alice F. Tarantal
Stephanie Groman
Richard E. Carson
Publikationsdatum: 28.05.2022
Verlag: Springer Berlin Heidelberg
Erschienen in: European Journal of Nuclear Medicine and Molecular Imaging / Ausgabe 11/2022
Print ISSN: 1619-7070
Elektronische ISSN: 1619-7089
DOI: https://doi.org/10.1007/s00259-022-05825-6

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Imaging the fetal nonhuman primate brain with SV2A positron emission tomography (PET)

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

Purpose

Methods

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Conclusion

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