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06.01.2021 | Original Research Paper | Ausgabe 2/2021

Inflammation Research 2/2021

Immuno-comparative screening of adult-derived human liver stem/progenitor cells for immune-inflammatory-associated molecules

Zeitschrift:
Inflammation Research > Ausgabe 2/2021
Autoren:
Makram Merimi, Laurence Lagneaux, Catherine A. Lombard, Douâa Moussa Agha, Dominique Bron, Philippe Lewalle, Nathalie Meuleman, Mustapha Najimi, Etienne M. Sokal, Mehdi Najar
Wichtige Hinweise
Responsible Editor: John Di Battista.
Makram Merimi and Laurence Lagneaux are equal contributors.

Publisher's Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Abstract

Objective

One of the main challenges in liver cell therapy is the replacement of damaged cells and the induction of a tolerogenic microenvironment to promote graft acceptance by the recipient. Adult-derived human liver stem/progenitor cells (ADHLSCs) are currently evaluated at the clinical levels as a promising pro-regenerative and immune-modulatory tool. The expression profile of several immunological molecules may influence the local immune-inflammatory response and, therefore, modulate the tissue healing process. To increase the quality and safety of ADHLSCs before transplantation requires an appropriate analysis and characterization of their pattern expression of immune-inflammatory-associated molecules.

Methods

The expression of 27 molecules belonging to T-cell co-stimulatory pathway, CD47 partners, Ikaros family, CD300 family and TNF family were analyzed using flow cytometry. We compared their expression profiles to PBMCs, hepatocytes and ADHLSCs in both expansion and after hepatogenic differentiation culture conditions.

Results

This original immuno-comparative screening revealed that liver cell populations do not constitutively present significant immunological pattern compared to PBMCs. Moreover, our findings highlight that neither the expansion nor the hepatogenic differentiation induces the expression of immune-inflammatory molecules. The detailed expression characteristics (percentage of positive cells and median fluorescence intensity) of each molecule were analyzed and presented.

Conclusion

By analyzing 27 relevant molecules, our immuno-comparative screening demonstrates that ADHLSCs keep a non-immunogenic profile independent of their expansion or hepatogenic differentiation state. Accordingly, the immunological profile of ADHLSCs seems to support their safe and efficient use in liver tissue therapeutic repair strategy.

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