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European Archives of Oto-Rhino-Laryngology

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22.08.2019 | Head and Neck

Immunohistochemical analysis of MMP-13 and EMMPRIN in epithelial odontogenic lesions

verfasst von: Pedro Paulo de Andrade Santos, Cassiano Francisco Weege Nonaka, Carlos Augusto Galvão Barboza, Leão Pereira Pinto, Lélia Batista de Souza

Erschienen in: European Archives of Oto-Rhino-Laryngology | Ausgabe 11/2019

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Abstract

Purpose

To investigate the contribution of MMP-13 in tumor aggressiveness, by acting on the reorganization of the extracellular matrix, regulating the biological activity of cytokines in odontogenic epithelial lesions, as well as to evaluate the role of EMMPRIN as an inducer of MMP-13.

Methods

Twenty solid ameloblastomas (SAs), 10 unicystic ameloblastomas (UAs), 20 odontogenic keratocysts (OKCs), and 20 adenomatoid odontogenic tumors (OATs) were selected. The expression of MMP-13 and EMMPRIN was evaluated in epithelial/connective tissue by determining the score of immunoreactive cells.

Results

Higher concentration of MMP-13 was observed in epithelium of SAs and OKCs (p = 0.316), while in connective, MMP-13 was more expressed in OKCs and UAs (p = 0.213). OKCs exhibited the highest immunoreactivity score for EMMPRIN in the epithelium (p = 0.091). In connective tissue, a larger number of immunoreactive cells were observed in OKCs and UACs (p = 0.357). There was a moderate correlation (r = 0.343/p = 0.004) between MMP-13/EMMPRIN in epithelium and strong correlation (r = 0.474/p < 0.001) in connective tissue.

Conclusion

We suggest that the OKCs, SAs and UAs presented greater immunoexpression for MMP-13 and EMMPRIN, since they were lesions of more aggressive behavior, with smaller expressions in the AOTs that are admittedly indolent. However, we did not find a statistically significant difference between the expression of MMP-13 and EMMPRIN in lesions studied. The positive correlation found between MMP-13 and EMMPRIN in the epithelial and connective tissue of odontogenic lesions analyzed, seems to be related to the role of EMMPRIN as an inducer of MMP-13 expression.

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Titel: Immunohistochemical analysis of MMP-13 and EMMPRIN in epithelial odontogenic lesions
verfasst von: Pedro Paulo de Andrade Santos
Cassiano Francisco Weege Nonaka
Carlos Augusto Galvão Barboza
Leão Pereira Pinto
Lélia Batista de Souza
Publikationsdatum: 22.08.2019
Verlag: Springer Berlin Heidelberg
Erschienen in: European Archives of Oto-Rhino-Laryngology / Ausgabe 11/2019
Print ISSN: 0937-4477
Elektronische ISSN: 1434-4726
DOI: https://doi.org/10.1007/s00405-019-05589-0

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Immunohistochemical analysis of MMP-13 and EMMPRIN in epithelial odontogenic lesions

Abstract

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Methods

Results

Conclusion

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