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Inflammation
Erschienen in: Inflammation 3/2018

23.02.2018 | ORIGINAL ARTICLE

Immunomodulatory Effects of CP-25 on Splenic T Cells of Rats with Adjuvant Arthritis

verfasst von: Yang Wang, Chen-chen Han, Dongqian Cui, Ting-ting Luo, Yifan Li, Yuwen Zhang, Yang Ma, Wei Wei

Erschienen in: Inflammation | Ausgabe 3/2018

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Abstract

Rheumatoid arthritis (RA) is an autoimmune disease in which T cells play an important role. Paeoniflorin-6-oxy-benzenesulfonate (CP-25) shows a strong anti-inflammatory and immunomodulatory effect in the joint of adjuvant arthritis (AA) rats, but the role of the spleen function is still unclear. The aim of this study was to research how CP-25 regulated spleen function of AA rats. Male Sprague-Dawley rats were administered with CP-25 (50 mg/kg) orally from day 17 to 29 after immunization. The spleen histopathological changes were analyzed by hematoxylin–eosin staining. G protein-coupled receptor kinases (GRKs) and prostaglandin receptor subtypes (EPs) were screened by Western blot and immunohistochemistry. The co-expression of GRK2 and EP2 as well as GRK2 and EP4 was measured by immunofluorescence and co-immunoprecipitation. The expression of GRK2 and EP4 in splenic T cells was further detected by immunofluorescence. CP-25 was found to relieve the secondary paw swelling, attenuate histopathologic changes, and downregulate GRK2, EP2 and EP4 expression in AA rats. Additionally, CP-25 not only downregulated the co-expression of GRK2 and EP4 but also downregulated GRK2, EP4 expression in splenic T cells of AA rats. From these results, we can infer that CP-25 play an anti-inflammatory and immune function by affecting the function of the splenic T cells.
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Metadaten
Titel
Immunomodulatory Effects of CP-25 on Splenic T Cells of Rats with Adjuvant Arthritis
verfasst von
Yang Wang
Chen-chen Han
Dongqian Cui
Ting-ting Luo
Yifan Li
Yuwen Zhang
Yang Ma
Wei Wei
Publikationsdatum
23.02.2018
Verlag
Springer US
Erschienen in
Inflammation / Ausgabe 3/2018
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-018-0757-z

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