Skip to main content
Erschienen in: Comparative Clinical Pathology 6/2020

21.10.2020 | Original Article

Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms

verfasst von: Juliana E. Bezerril, Paulo F. Marcusso, Gustavo S. Claudiano, Jefferson Yunis-Aguinaga, Thalita R. Petrillo, Antonio C. Alessi

Erschienen in: Comparative Clinical Pathology | Ausgabe 6/2020

Einloggen, um Zugang zu erhalten

Abstract

The aim of this study was to investigate glycoconjugate immunostaining of the biotinylated lectins: UEA - Ulex europaeus (gorse), PNA - Arachis hypogaea (peanut), HPA - Helix pomatia (Roman snail), and DBA - Dolichos biflorus, in normal mammary glands (control; n = 7), mammary neoplasms (n = 111), and mammary neoplasm metastasis (n = 10). One hundred twenty-eight mammary gland tissue samples were used; 7 samples without history of neoplasms (control). The remaining samples (121) were mammary neoplasms or metastasis. In all cases (UEA, HPA, PNA, and DBA), the binding to normal epithelium was uniform. The immunohistochemical marking was in the membrane and cytoplasm of epithelial cells in most of the cases. Large variations of binding between cell membranes, cytoplasm, and nuclei occurred in the neoplastic and metastatic tissues. Though lacking statistical differences, UEA, PNA, HPA, and DBA lectins revealed different marking patterns between tissues, being metastatic foci less marked in comparison with normal and neoplastic mammary gland tissues. In addition, there were no significant differences in the immunostaining of lectins between tumors where HPA was the most expressed in papillary carcinoma I, PNA in papillary carcinoma II, DBA in carcinosarcoma, and HPA and DBA in mixed carcinoma. It was concluded that the lectins have diagnostic potential and they have been useful for differentiation between normal and neoplastic breast tissue of metastatic foci in mammary tumor in dogs.
Literatur
Zurück zum Zitat Cassali GD, Lavalle GE, de Nardi AB, Ferreira E et al (2011) Consensus for the diagnosis, prognosis and treatment of canine mammary tumors. ABPV 4:131–142 Cassali GD, Lavalle GE, de Nardi AB, Ferreira E et al (2011) Consensus for the diagnosis, prognosis and treatment of canine mammary tumors. ABPV 4:131–142
Zurück zum Zitat Castagnaro M, Canese MG (1990) Lectin histochemical characteristics of the canine female mammary gland. Am J Vet Res 51:1815–1819PubMed Castagnaro M, Canese MG (1990) Lectin histochemical characteristics of the canine female mammary gland. Am J Vet Res 51:1815–1819PubMed
Zurück zum Zitat Manuali E, Giuseppe A, Feliziani F et al (2012) CA 15-3 cell lines and tissue expression in canine mammary cancer walk the correlation between serum levels and tumor histológica grid. BMC Vet Res. 8:246–261CrossRef Manuali E, Giuseppe A, Feliziani F et al (2012) CA 15-3 cell lines and tissue expression in canine mammary cancer walk the correlation between serum levels and tumor histológica grid. BMC Vet Res. 8:246–261CrossRef
Zurück zum Zitat Misdorp H, Else R, Hellman E (1999). Histologic classification of mammary tumors of the dog and cat. 1999 In: World Health Organization Histológica International Classification of Tumors of Domestic Animals 2 Ed. Armed Forces Institute of Pathology Washington DC. Misdorp H, Else R, Hellman E (1999). Histologic classification of mammary tumors of the dog and cat. 1999 In: World Health Organization Histológica International Classification of Tumors of Domestic Animals 2 Ed. Armed Forces Institute of Pathology Washington DC.
Zurück zum Zitat Nangia-Makker P, Hogan V, Honjo Y et al (2002) Stage of human cancer cell growth and metastasis in nude mice by oral intake of modified citrus. PectinJ Natl Cancer Inst 94:24 Nangia-Makker P, Hogan V, Honjo Y et al (2002) Stage of human cancer cell growth and metastasis in nude mice by oral intake of modified citrus. PectinJ Natl Cancer Inst 94:24
Zurück zum Zitat Ordofiez NG, Batsakis JG (1984) Comparison of Ulex europaeus I lectin and factor VIII-related antigen in vascular lesions. Arch Pathol Lab Med. 108:129–132 Ordofiez NG, Batsakis JG (1984) Comparison of Ulex europaeus I lectin and factor VIII-related antigen in vascular lesions. Arch Pathol Lab Med. 108:129–132
Zurück zum Zitat Sams J, Lynch HT, Burt RW (1990) Lectin histochemistry of abnormalities in Familial Polyposis Coli and hereditary nonpolyposis colorectal cancer. Cancer. 66:502–508CrossRef Sams J, Lynch HT, Burt RW (1990) Lectin histochemistry of abnormalities in Familial Polyposis Coli and hereditary nonpolyposis colorectal cancer. Cancer. 66:502–508CrossRef
Zurück zum Zitat Skutelsky E, Alroy J, Ucci AA, Carpenter JL (1987) Moore FM (1987) Modulation of carbohydrate residues in regenerative nodules and neoplasms of canine and feline pancreas. Am. J. Clin. Pathol. 126:25–31 Skutelsky E, Alroy J, Ucci AA, Carpenter JL (1987) Moore FM (1987) Modulation of carbohydrate residues in regenerative nodules and neoplasms of canine and feline pancreas. Am. J. Clin. Pathol. 126:25–31
Zurück zum Zitat Snedecor GW, Cochran WG (1974) Statistical methods. Ames, Iowa State College, p 117 Snedecor GW, Cochran WG (1974) Statistical methods. Ames, Iowa State College, p 117
Zurück zum Zitat Varki A (1993) Biological roles of oligosaccharides – all of the theories are correct. Glycobiology 3:97–130CrossRef Varki A (1993) Biological roles of oligosaccharides – all of the theories are correct. Glycobiology 3:97–130CrossRef
Metadaten
Titel
Immunostaining of lectin glycoconjugates in primary and metastatic canine mammary neoplasms
verfasst von
Juliana E. Bezerril
Paulo F. Marcusso
Gustavo S. Claudiano
Jefferson Yunis-Aguinaga
Thalita R. Petrillo
Antonio C. Alessi
Publikationsdatum
21.10.2020
Verlag
Springer London
Erschienen in
Comparative Clinical Pathology / Ausgabe 6/2020
Print ISSN: 1618-5641
Elektronische ISSN: 1618-565X
DOI
https://doi.org/10.1007/s00580-020-03113-z

Weitere Artikel der Ausgabe 6/2020

Comparative Clinical Pathology 6/2020 Zur Ausgabe

Neu im Fachgebiet Pathologie