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Erschienen in: Odontology 4/2020

25.02.2020 | Original Article

Impact of non-surgical periodontal treatment on salivary expression of cytokines related to bone metabolism

verfasst von: Tatiane F. C. S. B. Beiler, João Martins de Mello Neto, Juliana Cardoso Alves, Stephen Hamlet, Deepak Ipe, Carlos Marcelo da Silva Figueredo

Erschienen in: Odontology | Ausgabe 4/2020

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Abstract

We aimed to evaluate the impact of non-surgical periodontal treatment on the salivary expression of leptin, TNF-α, sclerostin, parathyroid hormone, osteoprotegerin, osteopontin, osteocalcin, IL-6, IL-1β and fibroblast growth factor 23 in patients with chronic periodontitis after 1 year of follow-up. Fifteen patients with chronic periodontitis (56.0 ± SD 9.6 years) and 15 subjects with gingivitis (39.7 ± SD 4.4 years) were included in the study. Clinical periodontal parameters, such as probing pocket depth (PPD), clinical attachment level (CAL), % of plaque and bleeding on probing (BOP) were evaluated, and non-stimulated whole saliva was collected from all patients before periodontal treatment and after 1 year of follow-up. A bead-based multiplex assay measured cytokines. In the chronic periodontitis group, periodontal treatment significantly improved clinical parameters and reduced the salivary levels of IL-1β, leptin and TNF-α (p = 0.002, 0.007 and 0.015, respectively). In the gingivitis group, there were also significant improvements in the mean patient %BOP, % Plaque, CAL and PPD. However, there were no significant changes in the cytokine’s salivary levels. In conclusion, chronic periodontitis patients showed a significant reduction in the salivary levels of leptin, TNF-α and IL-1β 1 year after periodontal treatment and a significant improvement in their clinical periodontal parameters suggesting that periodontal treatment alone can downregulate important cytokines associated with bone metabolism.
Literatur
1.
Zurück zum Zitat Armitage GC. The complete periodontal examination. Periodontol. 2000;2004(34):22–33. Armitage GC. The complete periodontal examination. Periodontol. 2000;2004(34):22–33.
2.
Zurück zum Zitat Figueredo CM, Ribeiro MS, Fischer RG, Gustafsson A. Increased interleukin-1beta concentration in gingival crevicular fluid as a characteristic of periodontitis. J Periodontol. 1999;70:1457–63.CrossRef Figueredo CM, Ribeiro MS, Fischer RG, Gustafsson A. Increased interleukin-1beta concentration in gingival crevicular fluid as a characteristic of periodontitis. J Periodontol. 1999;70:1457–63.CrossRef
3.
Zurück zum Zitat Gursoy UK, Liukkonen J, Jula A, et al. Associations between salivary bone metabolism markers and periodontal breakdown. J Periodontol. 2016;87:367–75.CrossRef Gursoy UK, Liukkonen J, Jula A, et al. Associations between salivary bone metabolism markers and periodontal breakdown. J Periodontol. 2016;87:367–75.CrossRef
4.
Zurück zum Zitat Borges CD, Ricoldi MS, Messora MR, et al. Clinical attachment loss and molecular profile of inflamed sites before treatment. J Appl Oral Sci. 2019;27:e20180671.CrossRef Borges CD, Ricoldi MS, Messora MR, et al. Clinical attachment loss and molecular profile of inflamed sites before treatment. J Appl Oral Sci. 2019;27:e20180671.CrossRef
5.
Zurück zum Zitat Upadhyay J, Farr OM, Mantzoros CS. The role of leptin in regulating bone metabolism. Metabolism. 2015;64:105–13.CrossRef Upadhyay J, Farr OM, Mantzoros CS. The role of leptin in regulating bone metabolism. Metabolism. 2015;64:105–13.CrossRef
6.
Zurück zum Zitat Reid IR, Baldock PA, Cornish J. Effects of leptin on the skeleton. Endocr Rev. 2018;39:938–59.CrossRef Reid IR, Baldock PA, Cornish J. Effects of leptin on the skeleton. Endocr Rev. 2018;39:938–59.CrossRef
7.
Zurück zum Zitat Chen XX, Yang T. Roles of leptin in bone metabolism and bone diseases. J Bone Miner Metab. 2015;33:474–85.CrossRef Chen XX, Yang T. Roles of leptin in bone metabolism and bone diseases. J Bone Miner Metab. 2015;33:474–85.CrossRef
8.
Zurück zum Zitat Gordeladze JO, Drevon CA, Syversen U, Reseland JE. Leptin stimulates human osteoblastic cell proliferation, de novo collagen synthesis, and mineralization: impact on differentiation markers, apoptosis, and osteoclastic signalling. J Cell Biochem. 2002;85:825–36.CrossRef Gordeladze JO, Drevon CA, Syversen U, Reseland JE. Leptin stimulates human osteoblastic cell proliferation, de novo collagen synthesis, and mineralization: impact on differentiation markers, apoptosis, and osteoclastic signalling. J Cell Biochem. 2002;85:825–36.CrossRef
9.
Zurück zum Zitat Matarese G, La Cava A. The intricate interface between immune system and metabolism. Trends Immunol. 2004;25:193–200.CrossRef Matarese G, La Cava A. The intricate interface between immune system and metabolism. Trends Immunol. 2004;25:193–200.CrossRef
10.
Zurück zum Zitat Faggioni R, Fantuzzi G, Fuller J, Dinarello CA, Feingold KR. Grunfeld C IL-1 beta mediates leptin induction during inflammation. Am J Physiol. 1998;274:R204–8.PubMed Faggioni R, Fantuzzi G, Fuller J, Dinarello CA, Feingold KR. Grunfeld C IL-1 beta mediates leptin induction during inflammation. Am J Physiol. 1998;274:R204–8.PubMed
11.
Zurück zum Zitat Reis C, da Costa AV, Guimarães JT, et al. Clinical improvement following therapy for periodontitis: association with a decrease in IL-1 and IL-6. Exp Ther Med. 2014;8:323–7.CrossRef Reis C, da Costa AV, Guimarães JT, et al. Clinical improvement following therapy for periodontitis: association with a decrease in IL-1 and IL-6. Exp Ther Med. 2014;8:323–7.CrossRef
12.
Zurück zum Zitat Hokari T, Morozumi T, Komatsu Y, et al. Effects of antimicrobial photodynamic therapy and local administration of minocycline on clinical, microbiological, and inflammatory markers of periodontal pockets: a pilot study. Int J Dent. 2018;5:1748584. Hokari T, Morozumi T, Komatsu Y, et al. Effects of antimicrobial photodynamic therapy and local administration of minocycline on clinical, microbiological, and inflammatory markers of periodontal pockets: a pilot study. Int J Dent. 2018;5:1748584.
13.
Zurück zum Zitat Abiramidevi M, Nagappan V, Prasad PAK. Saliva—a tool for diagnosis in periodontitis. J Acad Dent. 2018;4:1–7.CrossRef Abiramidevi M, Nagappan V, Prasad PAK. Saliva—a tool for diagnosis in periodontitis. J Acad Dent. 2018;4:1–7.CrossRef
14.
Zurück zum Zitat Giannobile WV, Beikler T, Kinney JS, Ramseier CA, Morelli T, Wong DT. Saliva as a diagnostic tool for periodontal disease: current state and future directions Periodontol. 2000;2009(50):52–64. Giannobile WV, Beikler T, Kinney JS, Ramseier CA, Morelli T, Wong DT. Saliva as a diagnostic tool for periodontal disease: current state and future directions Periodontol. 2000;2009(50):52–64.
15.
Zurück zum Zitat Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999;4:1–6.CrossRef Armitage GC. Development of a classification system for periodontal diseases and conditions. Ann Periodontol. 1999;4:1–6.CrossRef
16.
Zurück zum Zitat Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci. 1993;20:72–7.CrossRef Navazesh M. Methods for collecting saliva. Ann N Y Acad Sci. 1993;20:72–7.CrossRef
17.
Zurück zum Zitat Sexton WM, Lin Y, Kryscio RJ, Dawson DR, Ebersole JL, Miller CS. Salivary biomarkers of periodontal disease in response to treatment. J Clin Periodontol. 2012;38:434–41.CrossRef Sexton WM, Lin Y, Kryscio RJ, Dawson DR, Ebersole JL, Miller CS. Salivary biomarkers of periodontal disease in response to treatment. J Clin Periodontol. 2012;38:434–41.CrossRef
18.
Zurück zum Zitat Öngöz Dede F, Balli U, Bozkurt Doğan Ş, Güven B. Interleukin-32 levels in gingival crevicular fluid and saliva of patients with chronic periodontitis after periodontal treatment. J Periodontal Res. 2017;52:397–407.CrossRef Öngöz Dede F, Balli U, Bozkurt Doğan Ş, Güven B. Interleukin-32 levels in gingival crevicular fluid and saliva of patients with chronic periodontitis after periodontal treatment. J Periodontal Res. 2017;52:397–407.CrossRef
19.
Zurück zum Zitat Yoshida RA, Gorjão R, Mayer MPA, et al. Inflammatory markers in the saliva of cerebral palsy individuals with gingivitis after periodontal treatment. Braz Oral Res. 2019;33:e033.CrossRef Yoshida RA, Gorjão R, Mayer MPA, et al. Inflammatory markers in the saliva of cerebral palsy individuals with gingivitis after periodontal treatment. Braz Oral Res. 2019;33:e033.CrossRef
20.
Zurück zum Zitat Purwar P, Khan MA, Gupta A, et al. The effects of periodontal therapy on serum and salivary leptin levels in chronic periodontitis patients with normal body mass index. Acta Odontol Scand. 2015;73:633–41.CrossRef Purwar P, Khan MA, Gupta A, et al. The effects of periodontal therapy on serum and salivary leptin levels in chronic periodontitis patients with normal body mass index. Acta Odontol Scand. 2015;73:633–41.CrossRef
21.
Zurück zum Zitat Graziani F, Cei S, Orlandi M, et al. Acute-phase response following full-mouth versus quadrant non-surgical periodontal treatment: a randomized clinical trial. J Clin Periodontol. 2015;42:843–52.CrossRef Graziani F, Cei S, Orlandi M, et al. Acute-phase response following full-mouth versus quadrant non-surgical periodontal treatment: a randomized clinical trial. J Clin Periodontol. 2015;42:843–52.CrossRef
22.
Zurück zum Zitat Torumtay G, Kırzıoğlu FY, Öztürk Tonguç M, Kale B, Calapoğlu M, Orhan H. Effects of periodontal treatment on inflammation and oxidative stress markers in patients with metabolic syndrome. J Periodontal Res. 2016;51:489–98.CrossRef Torumtay G, Kırzıoğlu FY, Öztürk Tonguç M, Kale B, Calapoğlu M, Orhan H. Effects of periodontal treatment on inflammation and oxidative stress markers in patients with metabolic syndrome. J Periodontal Res. 2016;51:489–98.CrossRef
23.
Zurück zum Zitat Lossdörfer S, Kraus D, Abuduwali N, Jäger A. Intermittent administration of PTH(1-34) regulates the osteoblastic differentiation of human periodontal ligament cells via protein kinase C- and protein kinase A-dependent pathways in vitro. J Periodontal Res. 2011;46:318–26.CrossRef Lossdörfer S, Kraus D, Abuduwali N, Jäger A. Intermittent administration of PTH(1-34) regulates the osteoblastic differentiation of human periodontal ligament cells via protein kinase C- and protein kinase A-dependent pathways in vitro. J Periodontal Res. 2011;46:318–26.CrossRef
24.
Zurück zum Zitat Bullon P, Chandler L, Segura Egea JJ, Perez Cano R, Martinez Sahuquillo A. Osteocalcin in serum, saliva and gingival crevicular fluid: their relation with periodontal treatment outcome in postmenopausal women. Med Oral Patol Oral Cir Bucal. 2007;12:E193–7.PubMed Bullon P, Chandler L, Segura Egea JJ, Perez Cano R, Martinez Sahuquillo A. Osteocalcin in serum, saliva and gingival crevicular fluid: their relation with periodontal treatment outcome in postmenopausal women. Med Oral Patol Oral Cir Bucal. 2007;12:E193–7.PubMed
25.
Zurück zum Zitat Balli U, Aydogdu A, Dede FO, Turer CC, Guven B. Gingival crevicular fluid levels of sclerostin, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in periodontitis. J Periodontol. 2015;86:1396–404.CrossRef Balli U, Aydogdu A, Dede FO, Turer CC, Guven B. Gingival crevicular fluid levels of sclerostin, osteoprotegerin, and receptor activator of nuclear factor-κB ligand in periodontitis. J Periodontol. 2015;86:1396–404.CrossRef
Metadaten
Titel
Impact of non-surgical periodontal treatment on salivary expression of cytokines related to bone metabolism
verfasst von
Tatiane F. C. S. B. Beiler
João Martins de Mello Neto
Juliana Cardoso Alves
Stephen Hamlet
Deepak Ipe
Carlos Marcelo da Silva Figueredo
Publikationsdatum
25.02.2020
Verlag
Springer Singapore
Erschienen in
Odontology / Ausgabe 4/2020
Print ISSN: 1618-1247
Elektronische ISSN: 1618-1255
DOI
https://doi.org/10.1007/s10266-020-00502-2

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