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08.07.2016 | Original Article | Ausgabe 5/2017

Clinical Oral Investigations 5/2017

Impact of the intermediary layer on sealant retention: a randomized 24-month clinical trial

Clinical Oral Investigations > Ausgabe 5/2017
Kelly Maria Silva Moreira, Kamila Rosamilia Kantovitz, Juliana Pedrini Dias Aguiar, Ana Flávia Sanches Borges, Fernanda Miori Pascon, Regina Maria Puppin-Rontani
Wichtige Hinweise
Kelly Maria Silva Moreira and Kamila Rosamilia Kantovitz contributed equally.



The aims of this study were to assess long-term impact of tooth eruption stages (ES) on sealant retention on occlusal surfaces previously coated with intermediary bonding layer and to determine caries prevention.

Materials and methods

Sixty-five school children were selected (aged 6–10 years), with four non-carious permanent first molar in different ES (OP (operculum present), ME (marginal edge), CE (completely erupted)). Split-mouth and single-blind study design was used. The teeth (260) were randomly selected according to treatment (sealant/technique): F (Fluroshield), H (Helioseal Clear Chroma), SF (Single Bond + F), EH (Excite + H). Sealant retention, marginal integrity, discoloration, and caries prevention were assessed after 6, 12, 18, and 24 months by calibrated examiner (Spearman = 0.91) using visual inspection. Data were submitted to the Cox proportional hazard model (survival analysis) and Likelihood ratio χ 2 test (correlation), p ≤ 0.05.


At baseline, ES was 20 % in OP, 54 % in ME, and 26 % in CE. There was no significant difference on sealant retention between the treatments (p = 0.2774). However, significant differences were found regarding the ES on sealant retention (p = 0.0041). The CE stage showed the highest retention survival rate during the 24 months. The overall sealant prevention average was found to be about 99.4 % and showed no difference between the groups.


Eruption stages affect sealant retention irregardless of the intermediate layer and type of sealant. However, there was caries prevention on tooth occlusal surfaces after 24 months, regardless of treatment.

Clinical relevance

Sealing is recommended to prevent occlusal caries of newly erupted teeth in high-caries-risk patients. However, its application is critical on moisture limited control surfaces independently of sealer material and technique.

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