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01.12.2014 | Research | Ausgabe 1/2014 Open Access

Fibrogenesis & Tissue Repair 1/2014

Impaired Cav-1 expression in SSc mesenchymal cells upregulates VEGF signaling: a link between vascular involvement and fibrosis

Zeitschrift:
Fibrogenesis & Tissue Repair > Ausgabe 1/2014
Autoren:
Paola Cipriani, Paola Di Benedetto, Daria Capece, Francesca Zazzeroni, Vasiliki Liakouli, Piero Ruscitti, Ilenia Pantano, Onorina Berardicurti, Francesco Carubbi, Edoardo Alesse, Roberto Giacomelli
Wichtige Hinweise

Electronic supplementary material

The online version of this article (doi:10.​1186/​1755-1536-7-13) contains supplementary material, which is available to authorized users.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

PC: study conception and design, data interpretation, literature search, figure creation, writing, paper revision, and acceptance; PDB: study conception and design, data interpretation, literature search, figure creation, writing, paper revision, and acceptance; DC: data collection, literature search, paper revision, and acceptance; FZ: data collection, literature search, paper revision and acceptance; VL: data collection, literature search, paper revision, and acceptance; PR: data collection, data interpretation, literature search, paper revision, and acceptance; IP: data collection, literature search, paper revision, and acceptance; OB: data collection, literature search, paper revision, and acceptance; FC: data collection, data interpretation, literature search, paper revision, and acceptance; EA: data collection, data interpretation, literature search, paper revision, and acceptance; RG: study design, data interpretation, writing, paper revision, and acceptance. All authors gave final approval for submitting the manuscript for review and agree to be accountable for all aspects of the work.

Abstract

Background

Systemic sclerosis (SSc) is characterized by vascular alteration and fibrosis, the former probably leading to fibrosis via the ability of both endothelial cells and pericytes to differentiate toward myofibroblast. It is well known that vascular endothelial growth factor A (VEGF-A, hereafter referred to as VEGF) may induce a profibrotic phenotype on perivascular cells. Caveolin-1 (Cav-1) is involved in the regulation of VEGF signaling, playing a role in the transport of internalized VEGF receptor 2 (VEGFR2) toward degradation, thus decreasing VEGF signaling. In this work, we assessed the levels of Cav-1 in SSc bone marrow mesenchymal stem cells (SSc-MSCs), a pericyte surrogate, and correlate these results with VEGF signaling, focusing onpotential pathogenic pathways leading to fibrosis.

Results

We explored the VEGF signaling assessing: (1) Cav-1 expression; (2) its co-localization with VEGFR2; (3) the activity of VEGFR2, by IF, immunoprecipitation, and western blot. In SSc-MSCs, Cav-1 levels were lower when compared to healthy controls (HC)-MSCs. Furthermore, the Cav-1/VEGFR2 co-localization and the ubiquitination of VEGFR2 were impaired in SSc-MSCs, suggesting a decreased degradation of the receptor and, as a consequence, the tyrosine phosphorylation of VEGFR2 and the PI3-kinase-Akt pathways were significantly increased when compared to HC. Furthermore, an increased connective tissue growth factor (CTGF) expression was observed in SSc-MSCs. Taken together, these data suggested the upregulation of VEGF signaling in SSc-MSCs. Furthermore, after silencing Cav-1 expression in HC-MSCs, an increased CTGF expression in HC-MSCs was observed, mirroring the results obtained in SSc-MSCs, and confirming the potential role that the lack of Cav-1 may play in the persistent VEGF signaling .

Conclusions

During SSc, the lower levels of Cav-1 may contribute to the pathogenesis of fibrosis via an upregulation of the VEGF signaling in perivascular cells which are shifted to a profibrotic phenotype.
Zusatzmaterial
Authors’ original file for figure 1
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Authors’ original file for figure 2
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Authors’ original file for figure 3
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Authors’ original file for figure 4
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Authors’ original file for figure 5
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Literatur
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