The online version of this article (doi:10.1186/1475-2875-11-103) contains supplementary material, which is available to authorized users.
The authors declare that they have no competing interests.
APW conceived of the study, participated in its design and co-ordination and helped draft the manuscript. RYO participated in the design of this study, carried out drug selection and molecular genetic studies and drafted the manuscript. NP generated a transgenic line and helped to draft the manuscript. All authors read and approved the final manuscript.
An improved methodology is presented here for transgenic Plasmodium berghei lines that express the negative selectable marker yFCU (a bifunctional protein that combines yeast cytosine deaminase and uridyl phosphoribosyl transferase (UPRT)) and substitutes delivery of selection drug 5-fluorocytosine (5FC) by intraperitoneal injection for administration via the drinking water of the mice. The improved methodology is shown to be as effective, less labour-intensive, reduces animal handling and animal numbers required for successful selection thereby contributing to two of the "three Rs" of animal experimentation, namely refinement and reduction.
Authors’ original file for figure 112936_2012_2088_MOESM1_ESM.jpeg
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- Improved negative selection protocol for Plasmodium berghei in the rodent malarial model
Rachael Y Orr
Andrew P Waters
- BioMed Central
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